We narrowed to 11,997 results for: pcDNA

1. pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

   Plasmid

   #181956

   Purpose

   Genetically encoded FRET-based cAMP indicator fused to AKAP79.

   Depositor

   Jin Zhang

   Article

   Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

   Insert

   AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
   

   Use

   Tags

   AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3.1 hSCN5AdeltaSIV GFP Nter

   Plasmid

   #145770

   Purpose

   expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

   Depositor

   Hugues Abriel

   Article

   Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

   Insert

   sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Stop codon at Serine 2013

   Promoter

   CMV

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-V5H6-mPCSK3 D153A

   Plasmid

   #122681

   Purpose

   Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)

   Depositor

   Liming Pei

   Article

   Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

   Insert

   Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed Aspartic Acid 153 to Alanine

   Promoter

   Available since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. LARG mtq sspB in pcDNA3.1

   Plasmid

   #90460

   Purpose

   RhoA mediated cell migration

   Depositor

   Narasimhan Gautam

   Article

   O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

   Inserts

   LARG DH-PH (aa 766–1138) (ARHGEF12 Human)
   mTq
   sspB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R73Q and deletion a.a. (234-239)

   Promoter

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-TO-C-8His-Flag

   Plasmid

   #157678

   Purpose

   C-terminal tagging vector

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   8His-Flag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pcDNA3-BG4-NLS-mVen

   Plasmid

   #211449

   Purpose

   mammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequence

   Depositor

   Helmut Pospiech

   Insert

   Anti-DNA G-quadruplex single chain antibody BG4
   

   Use

   Tags

   NLS-mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-Tras light chain

   Plasmid

   #216294

   Purpose

   Expresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-Pert Light chain

   Plasmid

   #216310

   Purpose

   Expresses anti-HER2 Pert Light chain in mammalian cells. To be paired with Pert heavy chain to form the anti-HER2 Pert Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Pert Light chain
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-FLAG-MTOR-F1888L

   Plasmid

   #69010

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR-F1888L (MTOR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   change Phenylalanine 1888 to Leucine

   Promoter

   Available since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-MTS-KD-c-Src-FLAG

    Plasmid

    #44653

    Depositor

    Yoshimi Homma

    Article

    Ogura et al Biochem J. 2012 Oct 15;447(2):281-9.

    Insert

    v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian) (SRC) (SRC Human)
    

    Use

    Tags

    FLAG tag and mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    changed lysine 298 to methionine

    Promoter

    CMV promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Venus-173-N-ORAI1

    Plasmid

    #87618

    Purpose

    N-terminal Venus (YFP) fragment fused to full-length ORAI1; for BiFC.

    Depositor

    Jin Zhang

    Article

    Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

    Insert

    Venus173-N-ORAI1 (ORAI1 Synthetic, Human)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), and Xpress(TM) ta…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA_SZ1-RGG-GFP-RGG

    Plasmid

    #124932

    Purpose

    Expression of SZ1-RGG-GFP-RGG

    Depositor

    Daniel Hammer, Matthew Good, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT-Flag-NRBP1

    Plasmid

    #48197

    Purpose

    Expressed flag-tagged NRBP1 (nuclear receptor binding protein 1)

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

    Insert

    NRBP1 (NRBP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1(+)_Strep-Lrrk2-Myc

    Plasmid

    #161582

    Purpose

    expression of full-length mouse Lrrk2 with C-terminal Myc tag

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Lyn-Akt-STOPS

    Plasmid

    #175009

    Purpose

    Plasma membrane-targeted Akt-STOPS.

    Depositor

    Jin Zhang

    Article

    Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

    Insert

    Lyn-mCherry-Akt-STOPS
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase and…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1553 pcDNA3 HA gamma adaptin 2

    Plasmid

    #10713

    Depositor

    William Sellers

    Insert

    Gamma adaptin 2 (AP1G2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

17. GLUT9-eGFP/pcDNA-DEST47

    Plasmid

    #18730

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    GLUT9-eGFP/pcDNA-DEST47 (SLC2A9 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pCDNA3.1(+)-myc-SNAP-GP135

    Plasmid

    #50376

    Purpose

    Express myc and SNAP-tagged GP135 in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    SNAP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA5-FRT/TO-LacR-TopBP1

    Plasmid

    #31313

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

    Insert

    TopBP1 (TOPBP1 Human)
    

    Use

    Tags

    FLAG and LacR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3.1 human cofilin S3A

    Plasmid

    #50854

    Purpose

    expresses constituvely active cofilin in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 S3A (CFL1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    constitutively active, S3A

    Promoter

    CMV

    Available since

    Feb. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits