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pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W

Plasmid

#67983

Purpose

Cas9 activity reporter (control) with GFP and BFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKGFP2ABFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE, modified BFP

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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GluN2B/NR2B glutamate receptor scFv [N59/20]

Plasmid

#182092

Purpose

Mammalian Expression of GluN2B/NR2B glutamate receptor scFv. Derived from hybridoma N59/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GluN2B/NR2B glutamate receptor (Rattus norvegicus) (Grin2b Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-HCN2 [N71/37R]

Plasmid

#177567

Purpose

Mammalian Expression Plasmid of anti-HCN2 (Rat). Derived from hybridoma N71/37.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-HCN2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Hcn2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Nav1.7 Na+ channel [N68/6R]

Plasmid

#177566

Purpose

Mammalian Expression Plasmid of anti-Nav1.7 Na+ channel (Human). Derived from hybridoma N68/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Nav1.7 Na+ channel (Homo sapiens) recombinant mouse monoclonal antibody (SCN9A Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Beta4-spectrin [N393/76R]

Plasmid

#177542

Purpose

Mammalian Expression Plasmid of anti-Beta4-spectrin (Human). Derived from hybridoma N393/76.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody (SPTBN4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

Plasmid

#113692

Purpose

U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 (NEWENTRY )
SaCas9 gRNA Cassette (NEWENTRY )

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

NLS

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-TrpV1 [N221/17R]

Plasmid

#177517

Purpose

Mammalian Expression Plasmid of anti-TrpV1 (Rat). Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpV1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Npas4 [N408/79]

Plasmid

#177547

Purpose

Mammalian Expression Plasmid of anti-Npas4 (Rat). Derived from hybridoma N408/79.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Npas4 (Rattus norvegicus) recombinant mouse monoclonal antibody (Npas4 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-7 [N183/15R]

Plasmid

#177507

Purpose

Mammalian Expression Plasmid of anti-QKI-7 (Human). Derived from hybridoma N183/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-QKI-7 (Homo sapiens) recombinant mouse monoclonal antibody (QKI Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Contactin/F3 [K73/20R]

Plasmid

#177447

Purpose

Mammalian Expression Plasmid of anti-Contactin/F3 (Rat). Derived from hybridoma K73/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Contactin/F3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Cntn1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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Pvalb-2A-Dre Flp-in replacement vector

Plasmid

#61571

Purpose

Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb-2A-Dre (Pvalb Mouse, D6 Bacteriophage)

Use

Recombinase-mediated cassette exchange using flp …

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Anti-NrCAM [N343/26R]

Plasmid

#177534

Purpose

Mammalian Expression Plasmid of anti-NrCAM (Mouse). Derived from hybridoma N343/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NrCAM (Mus musculus) recombinant mouse monoclonal antibody (Nrcam Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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B52 + CHEK2 sgSTOP

Plasmid

#100712

Purpose

B52 plasmid expressing CHEK2 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting CHEK2 (cloned using BbsI) (CHEK2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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B52 + FANCM sgSTOP

Plasmid

#100710

Purpose

B52 plasmid expressing FANCM sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting FANCM (cloned using BbsI) (FANCM Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-Calretinin [L122/6R]

Plasmid

#177468

Purpose

Mammalian Expression Plasmid of anti-Calretinin (Human). Derived from hybridoma L122/6.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Calretinin (Homo sapiens) recombinant mouse monoclonal antibody (CALB2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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Calbindin scFv [L109/57]

Plasmid

#182071

Purpose

Mammalian Expression of Calbindin scFv. Derived from hybridoma L109/57.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting Calbindin (Homo sapiens) (CALB1 Mouse)

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-GIT1 [N39B/8R]

Plasmid

#177545

Purpose

Mammalian Expression Plasmid of anti-GIT1 (Rat). Derived from hybridoma N39B/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GIT1 (Rattus norvegicus) recombinant mouse monoclonal antibody (Git1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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KI(GAPDH)-P2A-EGFP-CMV-NeoR

Plasmid

#114009

Purpose

HDR-cassette to target chicken GAPDH gene for EGFP expression under control of the endogenous promoter. The cassette contains NeoR gene for positive clone selection and DTA gene for negative selection

Depositor

Pavel Volchkov

Article

Antonova et al F1000Res. 2018 Feb 28;7:238. doi: 10.126

Inserts

GAPDH left arm (GAPDH Chicken)
eGFP
NeoR
GAPDH right arm

Expression

Mammalian

Promoter

-, CMV, and endogenous promoter (chicken GAPDH in…

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Collybistin [L120/12R]

Plasmid

#177575

Purpose

Mammalian Expression Plasmid of anti-Collybistin (Rat). Derived from hybridoma L120/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Collybistin (Rattus norvegicus) recombinant mouse monoclonal antibody (Arhgef9 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-GAD67 [L127/8R]

Plasmid

#177475

Purpose

Mammalian Expression Plasmid of anti-GAD67 (Human). Derived from hybridoma L127/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GAD67 (Homo sapiens) recombinant mouse monoclonal antibody (GAD1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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