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Addgene

We narrowed to 33,224 results for: tes

Showing: 4561 - 4580 of 33224 results
  1. GAMA-bio

    Plasmid
    #68522
    Purpose
    Expresses full-length P. vivax GAMA ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence
    Insert
    GAMA
    Use
    Tags
    rat CD4 d3+4, enzymatic biotinylation sequence
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pCDNA3_altPKA-Cα-mPAGFP

    Plasmid
    #114133
    Purpose
    Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.
    Depositor
    Insert
    Testis only splice variant of mouse PKA catalytic subunit α that has no myristoylation site, C-terminally tagged by mPAGFP (Prkaca Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. sgFFL_3x_siRNA

    Plasmid
    #59893
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sites
    Depositor
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--has three siRNA-like targets (fully c…
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
  4. PE-pegRNA(BG)-nsgRNA(BG)

    Plasmid
    #232727
    Purpose
    A dual-targeting vector that contains PE3 system guide RNAs against BFP (pegRNA(BG) and nsgRNA(BG)) with restriction enzyme sites for insertion of target site guide RNAS (pegRNAs(TS) and nsgRNA(TS)).
    Depositor
    Insert
    pegRNA(BG), nsgRNA(BG), pegRNAs(TS), nsgRNA(TS)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. p406 – TDH3-ER-FlucDM-GFP11-Ura

    Plasmid
    #177731
    Purpose
    DM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid with
    Depositor
    Insert
    FlucDM
    Use
    Tags
    GFP11, HA, HDEL, and KAR2 Signal Sequence
    Expression
    Bacterial and Yeast
    Mutation
    R188Q, R261Q (Destabilized Mutant)
    Promoter
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. sgFFL_d23

    Plasmid
    #59892
    Purpose
    synthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with two seed sites mutated
    Depositor
    Insert
    mCherry with intron containing the mouse mir-124–3
    Use
    Tags
    PEST and VAMP 3 UTR
    Expression
    Mammalian
    Mutation
    VAMP 3 UTR--3rd and fourth seed site removed
    Promoter
    doxycycline (Dox)-inducible promoter (CMV/TO)
    Available since
    April 1, 2015
    Availability
    Academic Institutions and Nonprofits only
  7. pcdna3 T7 Jip1b(471-707)(C478S and C699T)

    Plasmid
    #52132
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Insert
    JNK interacting protein-1b (JIP-1b)(471-707)(M1+6 ) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-470 and changed C478S and C6…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  8. pPKm-300

    Plasmid
    #104626
    Purpose
    Expresses pSIN - EF-1alpha - MTS - tFd, vector encoding mitochondrial-tagged Thermosynechococcus elongates Ferredoxin (Fd), under EF-1alpha promoter
    Depositor
    Insert
    tFd
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1alpha
    Available since
    Availability
    Academic Institutions and Nonprofits only
  9. pYTK095

    Plasmid
    #65202
    Purpose
    Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK system
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
  10. pYTK083

    Plasmid
    #65190
    Purpose
    Encodes AmpR-ColE1 as a Type 8 part to be used in the Dueber YTK system
    Depositor
    Insert
    AmpR-ColE1
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 4561 - 4580 of 33224 results