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HA-FOXO3a TM

Plasmid

#1788

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

T32A, S253A, S315A (no longer detectably phosphor…

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-OSH2-2xPH

Plasmid

#161987

Purpose

Mammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFP

Depositor

Tamas Balla, David Bryant

Article

Balla et al Mol Biol Cell. 2008 Feb;19(2):711-21. do

Insert

OSH2 (OSH2 Budding Yeast)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shControl-EGFP

Plasmid

#227685

Purpose

Expresses EGFP and scramble of shRNA targeting Atg7

Depositor

Cagla Eroglu

Article

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

scrambled shRNA control

Expression

Mammalian

Available Since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3,4,5)P3 Probe

Plasmid

#211514

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

BTK1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

Plasmid

#114595

Purpose

Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

Depositor

Brian Chow

Article

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3,4)P2 Probe

Plasmid

#211511

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4-Bisphosphate (PI(3,4)P2) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

TAPP1 cPHx3

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PH-FAPP1

Plasmid

#161986

Purpose

Expresses GFP-tagged PH-FAPP1. PI(4)P biosensor

Depositor

Tamas Balla, David Bryant

Article

Balla et al Mol Biol Cell. 2005 Mar;16(3):1282-95. d

Insert

PH-FAPP1 (PLEKHA3 Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_SypHer3s_cyto

Plasmid

#238971

Purpose

Mammalian neurons SypHer3s expression

Depositor

Dmitry Bilan

Article

Lanin et al J Biophotonics. 2020 Mar;13(3):e20190024

Insert

SypHer3s

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_GFAP_SypHer3s_cyto

Plasmid

#238969

Purpose

Mammalian astrocytes SypHer3s expression

Depositor

Dmitry Bilan

Article

Lanin et al J Biophotonics. 2020 Mar;13(3):e20190024

Insert

SypHer3s

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-PIPK1 gamma 90

Plasmid

#22299

Depositor

Pietro De Camilli

Article

Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

Insert

human phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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FLAG-Cdk9

Plasmid

#28100

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK5

Plasmid

#65244

Purpose

Expression of HA tagged ERK5 in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5 (MAPK7 Human)

Tags

HA

Expression

Mammalian

Mutation

A409T

Promoter

CMV

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSecTag-ATX

Plasmid

#17839

Depositor

Steven Rosen

Article

Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

Insert

Autotaxin (ENPP2 Human)

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
GFP-PIPK1 gamma 87

Plasmid

#22300

Depositor

Pietro De Camilli

Article

Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

Insert

human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pGex-Cav1

Plasmid

#33364

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

Cav1 (CAV1 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-dInPAkt(R23/25A)

Plasmid

#181835

Purpose

Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dInPAkt

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Arginines 23 and 25 in PH domain mutated to alani…

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pHJ423

Plasmid

#20681

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Thr 567 to Asp

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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FLAG-Cdk9 T186A

Plasmid

#28101

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Contains a T186A mutation in Cdk9

Available Since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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Grp1-PH pGEX2TK

Plasmid

#71379

Purpose

bacterial expression of PH domain for phosphoinositide binding assay

Depositor

Mark Lemmon

Article

Kavran et al J Biol Chem. 1998 Nov 13;273(46):30497-5

Insert

Grp1-PH (Cyth3 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 264-391

Promoter

tac

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-Lyn-dPlcR(K30/32L)

Plasmid

#181833

Purpose

Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dPlcR (K30L,K32L)

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-CRY2-OCRL

Plasmid

#79565

Purpose

expression of GFP-tagged CRY2PHR fused to inositol 5-phosphatase domain of OCRL

Depositor

Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

OCRL (OCRL Human)

Tags

CRY2 (photolyase homology region domain of Arabid…

Expression

Mammalian

Mutation

aa 234_539

Promoter

CMV

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10D(E215Q)-HA

Plasmid

#209248

Purpose

Mammalian expression of ATP10D mutant E215Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10D (ATP10D Human)

Tags

HA

Expression

Mammalian

Mutation

E215Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S277G

Plasmid

#13615

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Tags

FLAG

Expression

Mammalian

Mutation

Changed catalytic Serine 277 to Alanine

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pBS hBAF53a

Plasmid

#17879

Depositor

Jerry Crabtree

Article

Zhao et al Cell. 1998 Nov 25. 95(5):625-36.

Insert

BAF53a (ACTL6A Human)

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pIA1363

Plasmid

#166861

Purpose

Expression of codon optimized SARS-COV-2 Nsp8 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp8 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pIA1362

Plasmid

#166860

Purpose

Expression of codon optimized SARS-COV-2 Nsp7 in E. coli

Depositor

Irina Artsimovitch

Article

Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

Insert

SARS-COV-2 Nsp7 (ORF1ab Synthetic)

Tags

His-GB1

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSecTag-T210A-ATX

Plasmid

#17840

Depositor

Steven Rosen

Article

Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

Insert

T210A-ATX (ENPP2 Human)

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC

Plasmid

#22931

Purpose

Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)

Depositor

Alexandra Newton

Article

Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Insert

PHLPP1-deltaC (PHLPP1 Human)

Tags

HA

Expression

Mammalian

Mutation

PHLPP-deltaC, deletion of PDZ binding domain--the…

Promoter

CMV

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK5kin

Plasmid

#65245

Purpose

Expression of HA tagged ERK5 kinase domain in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5kin (MAPK7 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 410-817

Promoter

CMV

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only