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Stx5L(m55V)-GFP
Plasmid#209845PurposeExpresses Stx5 M55V mutant fused to GFP in mammalian cellsDepositorInsertSTX5 (STX5 Human)
UseTagseGFPExpressionMammalianMutationChanged Methionine 55 to Valine, causes loss of S…PromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
str-KDEL_Stx5L(M55V)-SBP-mCitrine
Plasmid#209846PurposeSynchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)DepositorInsertSTX5 (STX5 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCitrineExpressionMammalianMutationChanged Methionine 55 to Valine, causes loss of S…PromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
str-KDEL_Stx5S-SBP-mCitrine
Plasmid#154845PurposeSynchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)DepositorInsertStx5 (Short isoform) (STX5 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCitrineExpressionMammalianMutationLacks amino acids 1-54, enocdes short isoform of …PromoterCMVAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM587
Plasmid#133593PurposePart Plasmid for dCas9, Part 3b(coding sequence)DepositorInsertdCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM586
Plasmid#133592PurposePart Plasmid for dCas9, Part 3(coding sequence)DepositorInsertdCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM756
Plasmid#133600PurposePart Plasmid for ERG25 sgRNA, Part 234(promoter,coding region, terminator)DepositorInsertsgERG25
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM725
Plasmid#133594PurposePart Plasmid for Linus-Mxi, Part 4a(coding sequence)DepositorInsertLinus-Mxi1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM729
Plasmid#133598PurposePart Plasmid for Mxi1-dCas9, Part 3(coding sequence)DepositorInsertMxi1-dCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM767
Plasmid#133604PurposePart Plasmid for dCas9 NLS, Part 3(coding sequence)DepositorInsertdCas9 NLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM585
Plasmid#133591PurposePart Plasmid for dCas9, Part 3a(coding sequence)DepositorInsertdCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM768
Plasmid#133605PurposePart Plasmid for dCas9 NLS, Part 3b(coding sequence)DepositorInsertdCas9 NLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM769
Plasmid#133606PurposePart Plasmid for dCas9 NLS, Part 3a(coding sequence)DepositorInsertdCas9 NLS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM755
Plasmid#133599PurposePart Plasmid for ERG11 sgRNA, Part 234(promoter,coding region, terminator)DepositorInsertsgERG11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM764
Plasmid#133601PurposePart Plasmid for TEF1 sgRNA, Part 234(promoter,coding region, terminator)DepositorInsertsgTEF1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK3
Plasmid#122674PurposeExpresses mouse PCSK3 in mammalian cellsDepositorInsertMus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-p53 WT
Plasmid#49242Purposeexpresses human wt p53 and EGFP in mammalian cellsDepositorInsertp53 (TP53 Human)
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET41-MBP-t-A3A-t-His
Plasmid#109231PurposeBacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV proteaseDepositorInserthuman APOBEC3A (APOBEC3A Optimized for E. coli expression, Human)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*-XcP4H-MH4R
Plasmid#178043PurposeExpress green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTPDepositorInsertssuperfolder green fluorescent protein with TAG at 151 position
XcP4H with W179F mutation
dihydropterine reductase from human
pterin-4a-carbinolamine dehydratase from human
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB[U6-3 wex2 PUb-DsRed]
Plasmid#169010PurposeFor germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.DepositorInsertwhite ex2 sgRNA
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster U6:3Available sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only
