We narrowed to 1,216 results for: 5-Oct
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Stx5L(m55V)-GFP
Plasmid#209845PurposeExpresses Stx5 M55V mutant fused to GFP in mammalian cellsDepositorInsertSTX5 (STX5 Human)
UseTagseGFPExpressionMammalianMutationChanged Methionine 55 to Valine, causes loss of S…PromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
str-KDEL_Stx5L(M55V)-SBP-mCitrine
Plasmid#209846PurposeSynchronize trafficking of Stx5L (M55V) from the ER, for use with FLIM (RUSH system)DepositorInsertSTX5 (STX5 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCitrineExpressionMammalianMutationChanged Methionine 55 to Valine, causes loss of S…PromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
str-KDEL_Stx5S-SBP-mCitrine
Plasmid#154845PurposeSynchronize trafficking of Stx5S from the ER, for use with FLIM (RUSH system)DepositorInsertStx5 (Short isoform) (STX5 Human)
UseTagsStreptavidin Binding Protein (SBP) and mCitrineExpressionMammalianMutationLacks amino acids 1-54, enocdes short isoform of …PromoterCMVAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
NSK021 CMV-TO-p53R248Q (FLP-IN)
Plasmid#191167PurposeExpression of human p53 mutant R248Q in mammalian cellsDepositorInsertp53(R248Q) (TP53 Human)
UseTagsExpressionMammalianMutationR248QPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-p53 WT
Plasmid#49242Purposeexpresses human wt p53 and EGFP in mammalian cellsDepositorInsertp53 (TP53 Human)
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK3
Plasmid#122674PurposeExpresses mouse PCSK3 in mammalian cellsDepositorInsertMus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41-MBP-t-A3A-t-His
Plasmid#109231PurposeBacterial expression of A3A with N-terminal MBP an C-terminal His tag, both cleavable with TEV proteaseDepositorInserthuman APOBEC3A (APOBEC3A Optimized for E. coli expression, Human)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-sfGFP*-XcP4H-MH4R
Plasmid#178043PurposeExpress green fluorescent protein with TAG at 151 position and enzymes to biosynthesize 5HTPDepositorInsertssuperfolder green fluorescent protein with TAG at 151 position
XcP4H with W179F mutation
dihydropterine reductase from human
pterin-4a-carbinolamine dehydratase from human
UseTagsHis tagExpressionBacterialMutationPromoterAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB[U6-3 wex2 PUb-DsRed]
Plasmid#169010PurposeFor germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.DepositorInsertwhite ex2 sgRNA
UsePiggybacTagsExpressionInsectMutationPromoterDrosophila melanogaster U6:3Available sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJP-CoV2leader-nLuc-TSS
Plasmid#175430PurposeExpresses nLuc with 5' leader of CoV2 in mammalian cells.DepositorInsertnLUC plus 5' CoV2 leader
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCashGem-Xyl2-URA3-LINEAR
Plasmid#174840PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitisDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterENO1pAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-p53 tethered
Plasmid#49245Purposeexpresses two human WT p53s tethered together and also expresses EGFP in mammalian cellsDepositorInsertp53-p53 (TP53 Human)
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
NSK026 CMV-TO-p53R248W (FLP-IN)
Plasmid#191168PurposeExpression of human p53 mutant R248W in mammalian cellsDepositorInsertp53(R248W) (TP53 Human)
UseTagsExpressionMammalianMutationR248WPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-mPCSK8
Plasmid#122677PurposeExpresses mouse PCSK8 in mammalian cellsDepositorInsertMus musculus membrane-bound transcription factor peptidase, site 1 (Mbtps1), transcript variant 2 (Mbtps1 )
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpIMPDH
Plasmid#206980PurposeStreptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.DepositorInsertSpIMPDH (guaB Streptococcus pyogenes)
UseTagsHis tag (with TEV cleavage site)ExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
KM-CashGem-Xyl2-URA3-LINEAR
Plasmid#174838PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianusDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterTEF1pAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-eYFP-FKBP-MAD1-AA
Plasmid#114038PurposeChemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)DepositorInsertHuman MAD1 (MAD1L1 Human)
UseTagseYFP-FKBP12ExpressionMammalianMutationMAD2 binding deficient MAD1 K541A/L543APromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
T7-p73DD L371P-pcDNA3
Plasmid#23049DepositorInserttumor protein p73 (TP73 Human)
UseTagsT7ExpressionMammalianMutationtruncated cDNA encodes truncated protein (aa 327-…PromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
YL-CashGem-BAT1-URA3-LINEAR
Plasmid#174837PurposepRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolyticaDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterGPDpAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only
