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We narrowed to 14,415 results for: ESP

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Showing: 441 - 460 of 14415 results

  2. pCMV_NESPylRS(AF)_hU6tRNAPyl

    Plasmid
    #223508
    Purpose
    PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon
    Depositor
    Edward Lemke
    Article
    Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5
    Inserts
    NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
    tRNA Pyl
    Expression
    Mammalian
    Mutation
    Y306A; Y384F
    Promoter
    CVM, SP6 and U6
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    223508-plasmid-map-sequence-id-445174

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  5. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  6. mApple-CathespinB-6

    Plasmid
    #54871
    Purpose
    Localization: Lysosomes, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    CathespinB
    Tags
    mApple
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pX330-Flag-deSpCas9

    Plasmid
    #92114
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity eSpCas9 (1.1) (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive eSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A, K848A, K1003A, R1060A
    Promoter
    Cbh
    Available Since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92114-plasmid-map-sequence-id-197615

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  8. DNA-CARtcr-IRESpuro

    Plasmid
    #89346
    Purpose
    2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassette
    Depositor
    Ron Vale
    Article
    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do
    Insert
    TCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
    Use
    Lentiviral
    Tags
    SNAPf tag
    Expression
    Mammalian
    Promoter
    SFFV
    Available Since
    June 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    89346-plasmid-map-sequence-id-176306

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  11. mPlum-CathespinB-6

    Plasmid
    #55955
    Purpose
    Localization: Lysosomes, Excitation: 590, Emission: 649
    Depositor
    Michael Davidson
    Insert
    CathespinB (Ctsb Rat)
    Tags
    mPlum
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    55955-plasmid-map-sequence-id-173178

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  12. Dendra2-CathespinB-6

    Plasmid
    #57703
    Purpose
    Localization: Lysosomes, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    CathespinB
    Tags
    Dendra2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 441 - 460 of 14415 results