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pBXC3GH

Plasmid

#47070

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pICXNH3

Plasmid

#49020

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Promoter

AOX1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pRGD-KmR

Plasmid

#74108

Purpose

Plasmid containing kanamycin resistance gene cassette flanked by multiple restriction sites

Depositor

Hans-Martin Fischer

Article

Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

Insert

aphII

Use

Bacterial vector based on pmb1 replicon

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only

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pFA6a-natMX-tetO-Pcyc1-3xFLAG

Plasmid

#41021

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1-3xFLAG

Use

N-terminal tagging/promoter replacement

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-LexA

Plasmid

#26266

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

LexA

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pGGE009

Plasmid

#48841

Purpose

Provides the UBQ10 terminator as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

UBQ10 terminator

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX-tetO-Pcyc1-3xFLAG

Plasmid

#41022

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1-3xFLAG

Use

N-terminal tagging/promoter replacement

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pGGA012

Plasmid

#48818

Purpose

Provides the RPS5A promoter as GreenGate module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

RPS5A promoter

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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p214_LTJ_1kbCD90.1Template

Plasmid

#82667

Purpose

1kb HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

1kb genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pXCX.CMV.tTA.WPRE

Plasmid

#38057

Depositor

James Uney

Article

Lee et al J Gene Med. 2005 May;7(5):576-83.

Insert

tTA

Use

Adenoviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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p229_LTJ_2kbCD90.1Template

Plasmid

#82668

Purpose

2kb HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

2kb genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pICXC3H

Plasmid

#49022

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Promoter

AOX1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pXCX.TRE.EGFP.WPRE

Plasmid

#38060

Depositor

James Uney

Article

Lee et al J Gene Med. 2005 May;7(5):576-83.

Insert

EGFP

Use

Adenoviral

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pYEXC3GH

Plasmid

#49027

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCT-L7.5.1

Plasmid

#42900

Depositor

Dane Wittrup

Article

Hackel et al J Mol Biol. 2008 Sep 19;381(5):1238-52.

Insert

L7.5.1

Tags

HA and c-myc

Expression

Yeast

Promoter

Gal1-10

Available Since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR4-V5-2 (w234-1)

Plasmid

#17426

Purpose

Gateway entry vector for N-term V5 tag, frame B

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

V5

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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p248_LTJ_2kbCD45.1Template

Plasmid

#82662

Purpose

2kb HDR template for murine CD45.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

2kb genomic DNA sequence from murine CD45.1

Use

Hdr template for gene editing

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCG-B-SpyTag-C

Plasmid

#87373

Purpose

An ORF part-containing vector harbouring the SpyTag sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SpyTag

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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p213_LTJ_160bpCD90.1Template

Plasmid

#82666

Purpose

160bp HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

160bp genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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p230_LTJ_4kbCD90.1Template

Plasmid

#82669

Purpose

4kb HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

4kb genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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