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pCX:γB2ΔICD-EC5-VenusΔN3C9-P3

Plasmid

#209090

Purpose

mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

Depositor

Takeharu Nagai

Article

Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

Insert

PcdhgB2 (Pcdhgb2 Mouse)

Tags

PA tag

Expression

Mammalian

Promoter

Chicken β-actin

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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S10A mutant of H3S10ph biosensor

Plasmid

#120810

Purpose

FRET biosensor. Eliminating the H3 Serine 10 phosphorylation site in H3S10ph biosensor to abolish the detection capability

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa) S10A-YFP

Expression

Mammalian

Mutation

Serine 10 is mutated to Alanine, and Threonine 3,…

Promoter

CMV

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pLentiRhoA2G

Plasmid

#40179

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

RhoA Biosensor

Use

Lentiviral

Tags

His-Myc

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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Laconic/pcDNA3.1(-)

Plasmid

#44238

Purpose

a genetically-encoded sensor for lactate concentration, transport and metabolic flux

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

Insert

Laconic (LACtate Optical Nano Indicator from CECs)

Use

Lactate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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EKAR2G_design1_mTFP_wt_Venus_wt

Plasmid

#39813

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-mRuby3

Plasmid

#74252

Purpose

Mammalian expression of tandem fusion of mClover3-mRuby3

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

mClover3-mRuby3

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-mGFP-Actin

Plasmid

#21948

Depositor

Ryohei Yasuda

Article

Murakoshi et al Brain Cell Biol. 2008 Aug . 36(1-4):31-4

Insert

betaActin (ACTB Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Sept. 3, 2009

Availability

Academic Institutions and Nonprofits only

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mRuby2-C1

Plasmid

#54768

Purpose

Localization: C1 Cloning Vector, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

mRuby2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mRuby2-N1

Plasmid

#54614

Purpose

Localization: N1 Cloning Vector, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

mRuby2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pTriExRhoA2G

Plasmid

#40176

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

RhoA Biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-mKate2 caspase-3 biosensor

Plasmid

#37132

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange-DEVD-mKate2

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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mRuby2-Lifeact-7

Plasmid

#54674

Purpose

Localization: Actin, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Lifeact-mRuby2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-FLIM-AKAR

Plasmid

#63058

Purpose

Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backbone

Depositor

Bernardo Sabatini

Article

Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

Insert

FLIM-AKAR

Use

AAV

Expression

Mammalian

Promoter

EF-1α

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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Galphai2-mTurquoise2

Plasmid

#69621

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 2, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai2-mTurquoise2

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Galphai3-mTurquoise2

Plasmid

#69622

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 3, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai3-mTurquoise2

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Galphai1-mTurquoise2

Plasmid

#69620

Purpose

The heterotrimeric G-protein subunit, Gαi isoform 1, tagged with mTurquoise2

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

Galphai1-mTurquoise2

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
mRuby2-pBAD

Plasmid

#54771

Purpose

Localization: Protein Expression Vector , Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

6xHis and mRuby2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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dClover2-C1

Plasmid

#54577

Purpose

Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Type

Empty backbone

Tags

dClover2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pKanCMV-mRuby3-10aa-H2B

Plasmid

#74258

Purpose

Mammalian expression of N-terminal fusion of mRuby3 to H2B.

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

H2B

Tags

mRuby3

Expression

Mammalian

Promoter

CAG and CMV

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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EKARdual

Plasmid

#99870

Purpose

Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

Depositor

Marc Tramier

Article

Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

Insert

EKAR

Tags

ShadowG and mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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