We narrowed to 5,915 results for: SPI

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pLV-shScn4b-268

Plasmid

#99031

Purpose

Expresses a shRNA targeting the mouse Scn4b gene

Depositor

Amy Lasek

Article

Ji et al Front Cell Neurosci. 2017 Feb 13;11:17.

Insert

Scn4b shRNA (Scn4b Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pmCherry_C1 I3(resist)

Plasmid

#61399

Purpose

Expresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

mCherry

Expression

Mammalian

Mutation

silent mutations of bases C183T T192C T189C

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-GFP-I3(resist)

Plasmid

#61400

Purpose

Expresses GFP-tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

Inhibitor-3 (PPP1R11 Human)

Tags

GFP

Expression

Mammalian

Mutation

silent mutations of bases C183T T192C T189C

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-GFP-I3(V41A/W43A)

Plasmid

#61402

Purpose

Expresses GFP-tagged, PP1 binding-deficient Inhibitor-3 in mammalian cells

Depositor

Hemmo Meyer

Article

Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

Insert

inhibitor-3 (PPP1R11 Human)

Tags

GFP

Expression

Mammalian

Mutation

V41A/W43A

Promoter

CMV

Available Since

Sept. 25, 2015

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro shSlp4-a #2

Plasmid

#40070

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp4-a shRNA-2

Use

Lentiviral

Promoter

U6

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pLKO.1-puro shSlp4-a #1

Plasmid

#40069

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp4-a shRNA-1

Use

Lentiviral

Promoter

U6

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pmCTIP2-N106

Plasmid

#66808

Purpose

2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 with blasticidin resistance

Depositor

Andrew Yoo

Article

Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

Insert

CTIP2 isoform b (Bcl11b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Human EF1a

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pET14b His Rab8a Q67L

Plasmid

#186014

Purpose

His Rab8a Q67L

Depositor

Suzanne Pfeffer

Article

Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

Insert

His Rab8a Q67L (RAB8A Human)

Expression

Bacterial

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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aMHC-mCherry-Rex-Blasticidin

Plasmid

#21228

Depositor

Mark Mercola

Article

Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

Insert

alpha myosin heavy chain promoter

Use

Lentiviral

Tags

mCherry

Available Since

June 30, 2009

Availability

Academic Institutions and Nonprofits only

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pSPCas9(BB)-2A-GFP_ABCA3GFP_targeting

Plasmid

#188541

Purpose

Plasmid encoding pCas9 and gRNA targeting the endogenous human ABCA3 locus stop codon

Depositor

Darrell Kotton

Article

Sun et al Am J Respir Cell Mol Biol. 2021 Oct;65(4

Insert

gRNA

Use

CRISPR

Tags

GFP

Promoter

U6

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS2144

Plasmid

#104357

Purpose

AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter

Depositor

Elizabeth Simpson

Article

de Leeuw et al Mol Brain. 2016 May 10;9(1):52. doi: 10.

Insert

ssAAV-smCBA-EmGFP

Use

AAV

Promoter

smCBA

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGY577

Plasmid

#166663

Purpose

Light-inducible Cre recombinase (LiCre) lentiviral expression plasmid for light-induced Cre/Lox recombination

Depositor

Gaël Yvert

Article

Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

Insert

LiCre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

Cre: E340A, D341A

Promoter

CMV

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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PR_GEF (2XPDZ-mCherry-Larg (DH)

Plasmid

#80407

Purpose

Recruitable mCherry tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_mCherry_LARG DH (aa 766-997) (ARHGEF12 Human)

Tags

mCherry

Expression

Mammalian

Mutation

DH domain only (aa 766-997)

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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CAHS2parrc-mCh-2xFKBP (pBS1109)

Plasmid

#185304

Purpose

For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2parrc

Expression

Mammalian

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHEN-CoV2-nanobody-G6

Plasmid

#194589

Purpose

Bacterial expression of G6 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

Depositor

Gerald McInerney

Article

Hanke et al Sci Adv. 2022 Mar 25;8(12):eabm0220. doi

Insert

G6 (S )

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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PR_GEF YFP (2XPDZ-YFP-LARG DH)

Plasmid

#80408

Purpose

Recruitable YFP tagged RhoA GEF LARG (DH domain). Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

2XPDZ_YFP_LARG DH (aa 766-997) (ARHGEF12 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-DYRK1A

Plasmid

#101770

Purpose

Mammalian expression of DYRK1A fusion protein

Depositor

Michael Huen

Article

Li et al Nucleic Acids Res. 2024 Jul 5:gkae574. d

Insert

DYRK1A (DYRK1A Human)

Tags

S peptide-flag-streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

siRNA-resistant allele

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-eFFly-eGFP

Plasmid

#104834

Purpose

eFFly luciferase for invivo Imaging, eGFP for flourescent detection

Depositor

Irmela Jeremias

Article

Sandhofer et al Leukemia. 2015 Apr;29(4):828-38. doi: 10

Inserts

eFFly
eGFP

Use

Lentiviral and Luciferase; Egfp

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa (T286A)

Plasmid

#127390

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T286A)

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 286 to Alanine

Promoter

pCAG

Available Since

Jan. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-mEGFP-CaMKIIa (T305D/T306D)

Plasmid

#127611

Purpose

Fluorescent reporter for mutant Ca2+/calmodulin-dependent protein kinase II alpha (T305D/T306D)

Depositor

Ryohei Yasuda

Article

Chang et al Nat Commun. 2019 Jun 25;10(1):2784. doi:

Insert

calcium/calmodulin-dependent protein kinase II alpha (Camk2a Rat)

Tags

mEGFP

Expression

Mammalian

Mutation

changed Threonine 305/306 to Alanine

Promoter

pCAG

Available Since

Jan. 25, 2020

Availability

Academic Institutions and Nonprofits only

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