We narrowed to 39,884 results for: LAT;
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Plasmid#202423PurposeExpression of GFP-tagged PODXL without extracellular domainDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationdeleted extracellular domainPromoterAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
GAPDH
Plasmid#155622PurposeFor use in RBP tethering screenDepositorAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 CMV 3xFG eIF4G1 +MIC
Plasmid#158776PurposepcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.DepositorInserteIF4G1 (with microexon)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF6-Acly(S455D)
Plasmid#70768PurposeExpresses phosphomimetic mouse ATP-citrate lyase in mammalian cellsDepositorInsertATP Citrate Lyase (Acly Mouse)
UseTagsmyc-epitopeExpressionMammalianMutationS455DPromoterEF1alphaAvailable SinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
hGFAP-Cre
Plasmid#40591PurposeMouse astrocyte expression of CreDepositorInsertCre recombinase (GFAP Phage, Mouse, Human)
UseCre/Lox and Mouse TargetingTagsMP-1 fragment (mouse protamine 1 intron and polya…ExpressionMammalianMutationConstruct contains a nuclear-targeted Cre recombi…PromoterhGFAP promoterAvailable SinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF6-Acly(S455A)
Plasmid#70767PurposeExpresses phosphomutant mouse ATP-citrate lyase in mammalian cellsDepositorInsertATP Citrate Lyase (Acly Mouse)
UseTagsmyc-epitopeExpressionMammalianMutationS455APromoterEF1alphaAvailable SinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
ITGB1
Plasmid#51920PurposeExpresses full-length Integrin beta-1 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertITGB1 (ITGB1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationStop Codon at amino acid 728, before CD4, bio and…PromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-PBZ-4A-VC
Plasmid#110649Purposeexpresses a PAR-binding (PBZ) domain fused to venus C-terminus, contains point mutations that abolish PAR bindingDepositorInsertPBZ domain from APLF, amino acids 371-451 (APLF Human)
UseTagsHA and VCExpressionMammalianMutationC379A, C385A, C421A and C427APromoterCMVAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-SIN-BX SpyCatcher003-(short)mCD80
Plasmid#210567PurposeExpresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domainDepositorInsertSpycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable SinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-FLAG-AKAP-Lbc
Plasmid#67571PurposeExpresses GFP and FLAG-tagged AKAP-Lbc in mammalian cellsDepositorInsertA-kinase anchoring protein Lbc (AKAP13 Human)
UseTagsFLAG and GFPExpressionMammalianMutationR2604G in AKAPPromoterAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCXIZ GFP PODXL delta IC
Plasmid#202422PurposeExpression of GFP-tagged PODXL without intracellular domainDepositorInsertPODXL (PODXL Human)
UseLentiviralTagsGFPExpressionMutationdeleted intracellular domainPromoterAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-9K0Q
Plasmid#221739PurposeStable expression of GFP-9K0Q in mammalian cells to monitor membrane chargeDepositorInsertKRAS (KRAS Human)
UseRetroviralTagsGFPExpressionMammalianMutationdeleted amino acids 1-170PromoterAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP-GFP-shch-TOG
Plasmid#69113PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationSilent mutations to confer shRNA resistance.PromoterCMVAvailable SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin
Plasmid#99277PurposeDual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistanceDepositorInsertsH2B-mOrange2-IRES-EGFP
Puro
UseLentiviralTagsEGFP, H2B, IRES, PGK, and mOrange2ExpressionMutationPromoterEF1 alpha-human and PGK-mouseAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase
Plasmid#196185PurposeCo-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-HisDepositorInsertsUseTagsGST, HA, His6, and MBPExpressionBacterialMutationchanged Serine 9 to AlaninePromoterTacAvailable SinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5
Plasmid#97044PurposeExpresses LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells. *Please see notes below on P2A cleaving efficiency.DepositorInsertLigand Dependent Nuclear Receptor Corepressor, Runt-related transcription factor 1, ETS-related gene
UseLentiviralTagsExpressionMammalianMutationPromoterTet onAvailable SinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-Ku70-siR-Mut6E
Plasmid#82330PurposePlasmid for constitutive or doxy-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.DepositorInsertKu70 (XRCC6 Human)
UseTagsHAExpressionMammalianMutationMut6E corresponding to K282E K287E T300E K331E K3…PromoterCMV-tetAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-NR4A3-P2A-mCherry
Plasmid#203863PurposeTet-inducible lentiviral plasmid expressing NR4A3-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorAvailable SinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-O-FUW-NR4A1-P2A-mCherry
Plasmid#203859PurposeTet-inducible lentiviral plasmid expressing NR4A1-P2A-mCherry, with ORF specific barcode close to 3'LTR siteDepositorAvailable SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT3-EFa1-HA-Jag1
Plasmid#46051DepositorAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only