We narrowed to 16,622 results for: puromycin

1. pM378

   Plasmid

   #173174

   Purpose

   Expresses C9-1 gRNA-12xMBS from hU6 promoter

   Depositor

   Hajin Kim

   Article

   Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

   Insert

   C9-1 targeting gRNA-12xMBS
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-SIRT6-H133Y

   Plasmid

   #102327

   Purpose

   Human SIRT6 H133Y for mammalian expression

   Depositor

   Hening Lin

   Article

   Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   changed histidine 133 to tyrosine

   Promoter

   CMV

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-IP-PEX3-EGFP

   Plasmid

   #228142

   Purpose

   Expresses PEX3-GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

   Insert

   PEX3 (PEX3 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. BII-Sh-FUCCI

   Plasmid

   #133363

   Purpose

   Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Clover-Geminin-IRES-mKO2-Cdt1
   

   Tags

   HA-tagged Clover-Geminin

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRX-IPU-FLAG-NEK9

   Plasmid

   #168269

   Purpose

   Expresses NEK9 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   NEK9 (NEK9 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti_CMV:Flag-GpNLuc

   Plasmid

   #208840

   Purpose

   Expresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

   Insert

   GpNLuc LumiFluor
   

   Use

   Lentiviral

   Tags

   1xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLVX-RT005-HaloGPM

   Plasmid

   #163385

   Purpose

   2nd generation lentiviral vector to express sHalotag-sGFP module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   Halotag-GFP-PDGFRtm-2A-mCherry (HaloGPM)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. CBX3 A6.5 gRNA

   Plasmid

   #90569

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CBX3

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CBX3 (Guide Designation A6.5)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Puro-iNotch1IC

   Plasmid

   #75338

   Purpose

   doxycycline inducible Notch1 intracellular domain gene over-expression

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

   Insert

   Notch1 Intracellular domain (Notch1 Mouse)
   

   Expression

   Mammalian

   Promoter

   TRE-tight

   Available Since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

    Plasmid

    #84247

    Purpose

    Expresses direct fusion VPR-Sp dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    VPR-tagBFP-Sp dCas9
    Puro
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    2xNLS (SV40), HA tag, VPR, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG and PGK

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. CBX3 A5.5 gRNA

    Plasmid

    #90568

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CBX3

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CBX3 (Guide Designation A5.5)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Flag-HA-CYLD

    Plasmid

    #22544

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    CYLD (CYLD Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXS-IP Keima-Pex11B

    Plasmid

    #224558

    Purpose

    expresses Keima fused to PEX11B in mammalian cells

    Depositor

    William Kaelin, Gregory Wyant

    Article

    Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

    Insert

    PEX11B (PEX11B Human)
    

    Use

    Retroviral

    Tags

    Keima

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Donor_eGP2AP_RC

    Plasmid

    #133784

    Purpose

    Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

    Depositor

    Sriram Kosuri

    Article

    Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

    Type

    Empty backbone

    Tags

    eGFP-P2A-Puro

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQC.Flip-nluc(LgBiT1-8)CP[Casp3A]-Fluc

    Plasmid

    #200123

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[Casp3A] protease reporter and P2A firefly luciferase in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[Casp3A] P2A firefly luciferase
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV.ATMi

    Plasmid

    #14542

    Depositor

    Didier Trono

    Article

    Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

    Insert

    ATM shRNA (ATM Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    March 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. RRM1 E1.3 gRNA

    Plasmid

    #90880

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RRM1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    RRM1 (Guide Designation E1.3)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMXs-mPERK-FLAG-IP

    Plasmid

    #101793

    Purpose

    Retroviral vector encoding C-terminally FLAG tagged mouse PERK

    Depositor

    Seiichi Oyadomari

    Article

    Taniuchi et al Sci Rep. 2016 Sep 16;6:32886. doi: 10.10

    Insert

    Perk (Eif2ak3 Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-EF1A-Puro-mCherry-RAB7

    Plasmid

    #221555

    Purpose

    Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti_dCas9-2xAM

    Plasmid

    #92220

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA to be inserted into Bbs I sites
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available Since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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