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pARC8-LambdaRedBeta-EcSSB

Plasmid

#162572

Purpose

Arabinose inducible recombineering plasmid encoding Lambda-Red Beta with EcSSB for use with dsDNA templates and enhanced recombination efficiency.

Depositor

George Church

Publication

Filsinger et al Nat Chem Biol. 2021 Jan 18. pii: 10.1038

Inserts

Lambda Red-Beta
E. coli SSB

Expression

Bacterial

Promoter

pBAD

Available since

May 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-flag-GFP-iCAM-1

Plasmid

#205186

Purpose

This vector encodes of a synCAM (iCAM1) and GFP

Depositor

Wendell Lim

Publication

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 and GFP

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSR78

Plasmid

#90210

Purpose

Expresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyA

Depositor

Jeffrey Tabor

Publication

Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

Inserts

CcaS
Cph8
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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plk1 WT GFP-myc

Plasmid

#132963

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Publication

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1 (PLK1 Human)

Expression

Mammalian

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAVS1-Tet-OsTIR1(WT)-V5

Plasmid

#158663

Purpose

Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

Tet-OsTIR1(WT) AAVS1

Use

CRISPR

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SQ171fg/pCSacB

Plasmid

#69344

Purpose

SQ171 fast growing strain supported by pCSacB plasmid

Depositor

Michael Jewett

Publication

Orelle et al Nature. 2015 Jul 29. doi: 10.1038/nature

Insert

pCSacB

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{DTR-GFP}on-WPRE

Plasmid

#111395

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON DTR-EGFP (diphtheria toxin receptor for cell ablation)

Depositor

Adam Kepecs

Publication

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
DTR-EGFP

Use

AAV

Tags

EGFP and Myc

Expression

Mammalian

Promoter

hSynapsin

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoLSS-mKate2-Kan

Plasmid

#44943

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Expression

Yeast

Available since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2+cPLA2-mKate2

Plasmid

#162068

Purpose

Used to provide a steady-state readout of cPLA2 activation in live cells. Amino-terminally fused to mKate2 for red fluorescence.

Depositor

Philipp Niethammer

Publication

Enyedi et al Nat Cell Biol. 2013 Sep;15(9):1123-30. d

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Avian, xenopus, zebrafish

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pFGAMS-mCherry

Plasmid

#166024

Purpose

Expresses human PFAS-mCherry fusion in mammalian cells

Depositor

Stephen Benkovic

Publication

Doigneaux et al J Biol Chem. 2020 Jul 10;295(28):9551-95

Insert

PFAS-mCherry (PFAS Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pClathrin-miRFP670nano

Plasmid

#127430

Purpose

Clathrin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Publication

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano-clathrin light chain

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Launchpad (AVA2590)

Plasmid

#85442

Purpose

Lentiviral FRT-flanked EF1alfa-GFP PGK-PuromycinR cassette

Depositor

Joan Steitz

Publication

Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

Insert

FRT-flanked EF1alfa-GFP PGK-Puromycin cassette

Use

Lentiviral

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Lenti-EF1alpha-dCas9-p300_Blast

Plasmid

#192653

Purpose

3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Publication

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

dCas9-p300

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PB-REVERBA-mScarlet-NLS-PEST

Plasmid

#190658

Purpose

Fluorescent reporter for circadian rhythms

Depositor

Mary Teruel

Publication

Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

Insert

Reverba-mScarlet (Nr1d1 Mouse)

Expression

Mammalian

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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A2_uSFFV

Plasmid

#153415

Purpose

Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter

Depositor

Maki Nakayama

Publication

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

A*02:01-P2A-human beta2 microglobulin (B2M Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)

Plasmid

#138555

Purpose

Expresses human codon-optimized enhanced specificity SpCas9 and blasticidin resistance: EFS promoter-eSpCas9(1.1)-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Publication

Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

Insert

eSpCas9(1.1)

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Mutation

K848A, K1003A, R1060A

Promoter

EFS

Available since

June 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAH123

Plasmid

#66077

Purpose

The pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 version

Depositor

Tom Ellis

Publication

Ceroni et al Nat Methods. 2015 May;12(5):415-8. doi:

Insert

phi80 integrase

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMB22

Plasmid

#122244

Purpose

Lentivirus delivery for stable expression of AsCas12a-VPR

Depositor

Darjus Tschaharganeh

Publication

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Promoter

EFs

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBLADE(FP6*)-mCherry

Plasmid

#168048

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101* promoter. mCherry expression is controlled by the PBAD promoter.

Depositor

Barbara Di Ventura

Publication

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Inserts

Blue light-inducible AraC dimers in Escherichia coli
mCherry

Expression

Bacterial

Promoter

J23101* and PBAD

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-S1-GCaMP6f

Plasmid

#160722

Purpose

Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains GFP-based fluorescent reporter GCaMP6f (calcium indicator), Xpress tag, and S1 protein scaffold.

Depositor

Edward Boyden

Publication

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

S1-GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human ubiquitin C (UBC) promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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