We narrowed to 11,169 results for: ENA

1. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

   Plasmid

   #216283

   Purpose

   Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SpyCatcher003-TEVs-SpyTag003DA
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBIG1e

   Plasmid

   #80615

   Purpose

   pBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)

   Depositor

   Jan-Michael Peters

   Article

   Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. MTK Lentivirus Single Cassette Destination Vector (pAN2414)

   Plasmid

   #194220

   Purpose

   Backbone for constructing single casette expression plasmids using the Mammalian Tookit

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. VinculinTS-A50I/I997A

   Plasmid

   #111829

   Purpose

   Vinculin tension sensor with point mutations affecting ability to bind talin and actin

   Depositor

   Brenton Hoffman

   Article

   Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

   Insert

   VinculinTS (I997A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed vinculin alanine 50 to isoleucine and iso…

   Promoter

   CMV

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSmart-EF1a-Nla-cas11

   Plasmid

   #178882

   Purpose

   Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

   Depositor

   Yan Zhang

   Article

   Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

   Insert

   EF1a-NlaCas11c-bGH polyA
   

   Use

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHLP710

   Plasmid

   #203740

   Purpose

   Integration of OsTIR1-3xMyc at NEUT5L locus

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   OsTIR1
   

   Use

   Candida albicans integrating vector

   Tags

   3xMyc

   Expression

   Mutation

   Promoter

   ACT1 (C. albicans)

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMGS58

   Plasmid

   #135311

   Purpose

   A HygR-P2A-AID cassette to tag the protein of interest with auxin-inducible degron at the n-terminal

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   HygR-P2A-AID
   

   Use

   N terminal tagging plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-V5-hTEV

   Plasmid

   #65800

   Purpose

   expresses human codon-optimised TEV protease

   Depositor

   Douglas Green

   Article

   Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

   Insert

   V5-human TEV
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   codon-optimised to express in mammalian cells

   Promoter

   CMV

   Available since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pTCAV-FLEx(loxP)-FlpO

   Plasmid

   #67829

   Purpose

   Cre-dependent expression of FlpO from CAV2 virus

   Depositor

   Liqun Luo

   Article

   Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

   Insert

   FlpO
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-mCherry2-sgRNA (empty)

    Plasmid

    #198330

    Purpose

    Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    U6-sgRNA(F+E) empty
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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