We narrowed to 11,723 results for: Ski

1. gRNA-CXCR4(90)

   Plasmid

   #98962

   Purpose

   Mammalian expression plasmid for CRISPR gRNA targeting human CXCR4 exon 2

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCR4 (CXCR4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Encodes a gRNA for CRISPR-mediated targeting of C…

   Promoter

   U6

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX Bmx-SH2

   Plasmid

   #46409

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Bmx (BMX Human)
   

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 286-400

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1/NDUFS8/myc-His

   Plasmid

   #127489

   Purpose

   Expresses myc-tagged NDUFS8 in mammalian cells

   Depositor

   Shashi Jain, Dieter Wolf

   Article

   Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

   Insert

   NDUFS8 (NDUFS8 Human)
   

   Tags

   myc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCEP4-myc-GRM3-VC

   Plasmid

   #98968

   Purpose

   myc-tagged human mGluR3 fused to C-terminus of split Venus

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   GRM3 (GRM3 Human)
   

   Tags

   Signal/leader sequence from HLA class I A-2 alpha…

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV NucleoBAS N354K-I372V

   Plasmid

   #63574

   Purpose

   Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

   Depositor

   Stan van de Graaf

   Article

   van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

   Insert

   NucleoBAS N354K-I372V
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   N345K and I372V

   Promoter

   CMV

   Available Since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

   Plasmid

   #138525

   Purpose

   Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.

   Depositor

   Richard Jenner

   Article

   Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

   Insert

   Control non-PRC2 binding A-tract repeat RNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMXS-IRES-Blast SHMT2 CD

   Plasmid

   #106302

   Purpose

   Expresses SHMT2 K280A catalytic site mutant

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   serine hydroxymethyltransferase 2 (SHMT2 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   K280A catalytic site mutation, Silent mutations d…

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV.GFAP.SF-iGluSnFR.S72A

   Plasmid

   #106194

   Purpose

   Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-iGluSnFR.S72A
   

   Use

   AAV

   Mutation

   GltI: S72A

   Promoter

   GFAP

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. psiCHECK2-E2F1 3'UTR

   Plasmid

   #29468

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   E2F1 3'UTR (E2F1 Human)
   

   Expression

   Mammalian

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pCIBN-tagBFP-TetR

    Plasmid

    #103797

    Purpose

    BLInCR 'Localizer' construct that marks tetO arrays and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-TetR (CIB1, tetR Mustard Weed, E. coli)
    

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. psiCHECK2-p27Kip1 3'UTR

    Plasmid

    #29476

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    p27 Kip1 3'UTR (CDKN1B Human)
    

    Expression

    Mammalian

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

12. NAR4-CD4d3+4-bio

    Plasmid

    #73118

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding NAR4 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    NAR4 (ART4 Human)
    

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MKV937/nlp-1p::FlincG3

    Plasmid

    #140508

    Purpose

    This construct contains cGMP biosensor driven by nlp-1 promoter for expression in PHB sensory neuron in pSMdelta backbone.

    Depositor

    Miri VanHoven

    Article

    Woldemariam et al Genetics. 2019 Jul 22. pii: genetics.119

    Insert

    FlincG3
    

    Expression

    Worm

    Promoter

    nlp-1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Galectin3-CD4d3+4-bio

    Plasmid

    #73128

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Galectin3 with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    LGALS3 (LGALS3 Human)
    

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX307 DEDD

    Plasmid

    #117744

    Purpose

    Open reading frame vector encoding DEDD

    Depositor

    William Hahn

    Article

    Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

    Insert

    DEDD1 (DEDD Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO.1-shGBP1.1.mKO2

    Plasmid

    #85209

    Purpose

    TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    GBP1 (guanylate binding protein 1) (GBP1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAC4 Frk-SH2

    Plasmid

    #46428

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Frk (FRK Human)
    

    Tags

    AviTag, GST, and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 108-213

    Promoter

    Trc

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLJM1 Flag GFP 4E-BP1 Rheb15

    Plasmid

    #112761

    Purpose

    express GFP-4EBP1-Rheb15

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Rheb15 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Rheb15

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid

    #138524

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    PRC2-binding G-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXPR_BRD111

    Plasmid

    #78166

    Purpose

    Expresses Cas9 with the pLX311 backbone

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only