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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 4661 - 4680 of 6562 results

  3. pYPQ230-RR

    Plasmid
    #108860
    Purpose
    LbCpf1 Gateway entry plasmid with G548R and K611R mutations
    Depositor
    Yiping Qi
    Publication
    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do
    Insert
    LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
    Use
    CRISPR; Gateway compatible cpf1 entry clone
    Tags
    NLS
    Expression
    Plant
    Mutation
    G548R and K611R, Cpf1 is rice codon optimized
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    108860-plasmid-map-sequence-id-209932

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  4. pUBC_HA_PP7-mVenus

    Plasmid
    #111268
    Purpose
    Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag
    Depositor
    John Rinn
    Publication
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PP7-mVenus
    Use
    Synthetic Biology
    Tags
    HA, SV40 NLS, and mVenus
    Expression
    Mammalian
    Mutation
    PP7: C68S, S69G [delta]70-75 (non-aggregating)
    Promoter
    hUBC
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    111268-plasmid-map-sequence-id-212603

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  10. pX330-Flag-dSpCas9-HF1

    Plasmid
    #92115
    Purpose
    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive SpCas9-HF1 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, Q926A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92115-plasmid-map-sequence-id-197505

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  11. pLV-REPORT(EF1a)

    Plasmid
    #172326
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Publication
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172326-plasmid-map-sequence-id-354701

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  12. pLV-REPORT(PGK/CMV)

    Plasmid
    #172330
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Publication
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    PGK/CMV
    Available since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172330-plasmid-map-sequence-id-497743

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  14. eSpCas9-plus

    Plasmid
    #126767
    Purpose
    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126767-plasmid-map-sequence-id-410890

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  15. pAAV-MP-84-spCas9-U6-gRNA

    Plasmid
    #206935
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    MP-84
    Available since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    206935-plasmid-map-sequence-id-407781

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  17. YE1-BE4max-CP1028

    Plasmid
    #138160
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    138160-plasmid-map-sequence-id-272345

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Showing: 4661 - 4680 of 6562 results