167,318 results

1. pUC19-P70a-Top-T~P70a-Bottom-T

   Plasmid

   #87314

   Purpose

   Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   P70a

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. mOrange2-Fibrillarin-7

   Plasmid

   #57957

   Purpose

   Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   Fibrillarin (FBL Human)
   

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   R36Q, S66F

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Cyto-RA

   Plasmid

   #209860

   Purpose

   Dimerization dependent fluorescent protein RA fused to NES

   Depositor

   Sarah Cohen

   Article

   Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

   Insert

   ddRFP A
   

   Tags

   NES sequence NLVDLQKKLEELELDEQQ

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. p406GALL

   Plasmid

   #17434

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   GALL promoter is between SacI and XbaI restrictio…

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9_GFP

   Plasmid

   #44719

   Purpose

   Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. PJEBAN3

   Plasmid

   #203682

   Purpose

   Expresses Yellow Fluorescent Protein (YFP) in Listeria monocytogenes

   Depositor

   Tine Rask Licht

   Article

   Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

   Insert

   Yellow Fluorescent Protein (YFP)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GALT-SEP-mRuby3

   Plasmid

   #200938

   Purpose

   trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

   Depositor

   Nir Hacohen

   Article

   Liu et al Science. 2023 Aug 4;381(6657):508-514. d

   Inserts

   GalT (B4GALT1 Human)
   SEP-mRuby3
   

   Use

   Lentiviral

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSG5-PBX1a

   Plasmid

   #78149

   Purpose

   mammalian expression of PBX1a

   Depositor

   Francesco Blasi

   Insert

   PBX1 (PBX1 Human)
   

   Expression

   Mammalian

   Available Since

   June 10, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pSBtet-Bla/GFP

   Plasmid

   #198221

   Purpose

   Transposon vector (Addgene #60510) for doxycycline-inducible expression of EGFP

   Depositor

   Eric Davis

   Insert

   EGFP
   

   Use

   Transposon

   Expression

   Mammalian

   Mutation

   None

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_Gal4UAS_humanIL-2_IRES_mCherry_PGK_tBFP

    Plasmid

    #85426

    Purpose

    Lentiviral vector for Gal4 inducible humanIL-2 with mCherry reporter and constitutive tBFP expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    human IL-2
    

    Use

    Lentiviral

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p415-GalL-Cas9-CYC1t

    Plasmid

    #43804

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GalL

    Available Since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-HA-hTAF4b

    Plasmid

    #17998

    Depositor

    Robert Tjian

    Article

    Freiman et al Science. 2001 Sep 14. 293(5537):2084-7.

    Insert

    hTAF4b (TAF4B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa1-64 deleted

    Available Since

    May 28, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1 CARD8-TEV-GFP-FLAG S297A

    Plasmid

    #169992

    Purpose

    Mammalian purification construct for CARD8 with an autoprocessing-deficient mutation

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    TEV-siriusGFP-FLAG

    Expression

    Mammalian

    Mutation

    S297A

    Promoter

    CMV

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV_SEPT9_i5-msfGFP

    Plasmid

    #180327

    Purpose

    mammalian expression of human SEPT9_i5 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

    Insert

    SEPTIN9_v7 (SEPTIN9 Human)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBBR15.2-2H12.D11opt-scarRef

    Plasmid

    #221150

    Purpose

    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2.1
    mScarlet-I
    

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    Constitutive and None (same as cdGreen2.1)

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT-TODestFLAGHA_RBPMS2

    Plasmid

    #59390

    Purpose

    Destination vector with RBPMS2 for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS2 (RBPMS2 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    Feb. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMCB320

    Plasmid

    #89359

    Purpose

    3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    puromycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. cytoplasmic cpVenus control

    Plasmid

    #186788

    Purpose

    Identical to cytoplasmic NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    cytoplasmic cpVenus control
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.CAG.(ER).iGlucoSnFR2.HaloTag

    Plasmid

    #244073

    Purpose

    ER expression of green glucose sensor with non-responsive HaloTag

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    iGlucoSnFR2.HaloTag
    

    Use

    AAV

    Tags

    Calreticulin and HaloTag

    Available Since

    Sept. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.CAG.(mem).iGlucoSnFR2

    Plasmid

    #244077

    Purpose

    Plasma membrane targeted expression of green glucose sensor

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    iGlucoSnFR2
    

    Use

    AAV

    Tags

    IgG kappa leader

    Available Since

    Sept. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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