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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,065 results for: LEA

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Showing: 4681 - 4700 of 14065 results

  4. PANK1

    Plasmid
    #32871
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK1 (PANK1 Human)
    Tags
    His tag (with Thrombin cleavage)
    Expression
    Bacterial
    Mutation
    amino acids 231-597 only
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by

  9. pcDNA3-Mcm5-mCer

    Plasmid
    #211393
    Purpose
    mammalian expression of human Mcm5 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Mcm5 (MCM5 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211393-plasmid-map-sequence-id-420429

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  10. pDRFLIP37

    Plasmid
    #65515
    Purpose
    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.
    Depositor
    Wolf Frommer
    Publication
    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755
    Type
    Empty backbone
    Tags
    6His-Cit-attR1 and attR2-Cer-cMyc
    Expression
    Yeast
    Promoter
    pPMA1
    Available since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65515-plasmid-map-sequence-id-111425

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  11. tagSrc(WT)-mCer3 S208F/V224L

    Plasmid
    #179138
    Purpose
    mCerulean3 has S208F/V224L mutations that promote weak dimerization with YPet to optimize oritentation for FRET. tag is SsrA (AANDENY).
    Depositor
    Klaus Hahn
    Publication
    Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Src, SsrA
    Tags
    mCerulean3 (S208F/V224L)
    Expression
    Mammalian
    Available since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    179138-plasmid-map-sequence-id-353406

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  12. pOCC121

    Plasmid
    #118893
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    6xHis, cleavable with 3C protease and MBP, cleava…
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118893-plasmid-map-sequence-id-232270

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  19. pAAP2

    Plasmid
    #171208
    Purpose
    bacterial expression for purification of PGL-3, allowing cleavage for MBP tag using TEV protease
    Depositor
    Geraldine Seydoux
    Publication
    Schmidt et al bioRxiv 2020.10.15.340570
    Insert
    pgl-3 (pgl-3 Nematode)
    Tags
    MBP::HIS::TEV cleavage site
    Expression
    Bacterial
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171208-plasmid-map-sequence-id-335799

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Showing: 4681 - 4700 of 14065 results