We narrowed to 11,719 results for: Ski

1. S1 1029 CP ABEMax strategy 1

   Plasmid

   #135357

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1029 encoding ABEMax (wildtype Tada monomer coupled with evolved Tada monomer) at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   ABEMax-nSpyCas9 aa 1029
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. psiCHECK2-E2F3 3'UTR

   Plasmid

   #29470

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   E2F3 3'UTR (E2F3 Human)
   

   Expression

   Mammalian

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

3. psiCHECK2-CHEK1 3'UTR

   Plasmid

   #29478

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   CHEK1 3'UTR (CHEK1 Human)
   

   Expression

   Mammalian

   Available Since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pPN007

   Plasmid

   #91575

   Purpose

   Express sgRNA targeting human BCL11A

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   BCL11A (BCL11A Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCEP4-FLAG-CCR5-VN

   Plasmid

   #98963

   Purpose

   FLAG-tagged human CCR5 fused to N-terminus of split Venus

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CCR5 (CCR5 Human)
   

   Tags

   FLAG (dykdddd) epitope tag, Signal/leader sequenc…

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPHR-mCherry-NLS-VP16

   Plasmid

   #103821

   Purpose

   Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-mCherry-VP16 (CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   VP16: G126T (silent, G42G)

   Promoter

   CMV

   Available Since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPHR-iRFP713-NLS-VP16

   Plasmid

   #103823

   Purpose

   Soluble BLInCR transcription activator (transactivation domain of viral VP16 protein) that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-iRFP713-VP16 (CRY2 Mustard Weed)
   

   Expression

   Mammalian

   Mutation

   VP16: G126T (silent, G42G)

   Promoter

   CMV

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. integrin beta6 777T pcDNA1 neo

   Plasmid

   #13582

   Depositor

   Dean Sheppard

   Article

   Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

   Insert

   integrin beta 6 777T (ITGB6 Human)
   

   Expression

   Mammalian

   Mutation

   777T. Lacks last 11 amino acids of cytoplasmic do…

   Available Since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX FynT-SH2

   Plasmid

   #46430

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   FynB/FynT (FYN Human)
   

   Tags

   AviTag, GST, and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 142-251

   Promoter

   Tac

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. psiCHECK2-E2F2 3'UTR

    Plasmid

    #29469

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    E2F2 3'UTR (E2F2 Human)
    

    Expression

    Mammalian

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pCEP4-myc-CCR5-VC

    Plasmid

    #98966

    Purpose

    myc-tagged human CCR5 fused to C-terminus of split Venus

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe_puro_DEST_Flag_GPR160

    Plasmid

    #45274

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    GPR160 (GPR160 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pEGFP Kind2 S181E

    Plasmid

    #105312

    Purpose

    To study altered downstream functions of phospholylation-site mimetic kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S181E

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP Kind2 S181A

    Plasmid

    #105307

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S181A

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK5 GST MetaP2

    Plasmid

    #112750

    Purpose

    express HA-GST-FRB-MetaP2

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    HA-GST-FRB-MetaP2 (METAP2 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNAS1b split mCherry NEDD4 WW2 pos ctrl

    Plasmid

    #112029

    Purpose

    Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Insert

    NEDD4 WW2 domain fused to C-terminal split mCherry, proline-rich pos ctrl interacting peptide fused to N-terminal split mCherry
    

    Tags

    Split mCherry

    Expression

    Bacterial

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX Abl-SH2 RK

    Plasmid

    #46403

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Abl RK (Abl1 Mouse)
    

    Tags

    GST and Thrombin site

    Expression

    Bacterial

    Mutation

    contains amino acids 139-239

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX Jak1-SH2

    Plasmid

    #46447

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Jak1 (JAK1 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 430-552

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX PLCg2(NC)-SH2

    Plasmid

    #46472

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    PLCg2(NC) (PLCG2 Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 524-749

    Promoter

    Tac

    Available Since

    Aug. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPN186

    Plasmid

    #91573

    Purpose

    Express sgRNA targeting human AKT3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    AKT3 (AKT3 Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge