-
Plasmid#128495PurposeExpresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.DepositorInsertA2M (G27-S1468) (A2M Human)
UseTagsHis tag (6x)ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
PRNP-bio-His
Plasmid#51811PurposeExpresses full-length Major prion protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPRNP (PRNP Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-AC8(1-106)
Plasmid#181958PurposeResidues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.DepositorInsertEGFP-AC8(1-106) (Adcy8 Rat)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9
Plasmid#84244PurposeExpresses GA-inducible VPR-Sa dCas9DepositorInsertsGAI-tagBFP-Sa dCas9
GID1-VPR
UseCRISPR and Synthetic Biology; PiggybacTagsGAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …ExpressionMammalianMutationD10A, N580A and Synonymous mutation in PYL1 to re…PromoterCAG and PGKAvailable sinceNov. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_puromycin
Plasmid#65310PurposeLuminal ER hook only (RUSH system)DepositorInsertSTIM1 mutated for binding motif to microtubule (STIM1 Human)
UseTagsstreptavidinExpressionMammalianMutation2 amino acids of STIM1 (IP from SxIP motif) were …PromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYFP-ErbB2ΔC990
Plasmid#66946PurposeMammalian expression of rat ErbB2 ΔC990 tagged with YFPDepositorInsertErbB2 (Erbb2 Rat)
UseTags6xHis, V5 Tag, and YFPExpressionMammalianMutationDeletion of C990. (see depositor comment below on…PromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-WDR5
Plasmid#59969PurposeBacterial Expression GST-WDR5DepositorInsertWDR5 (WDR5 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK668
Plasmid#192637PurposeExpresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmRDepositorInsertsSp.pCas9-dCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)
UseCRISPR and Synthetic BiologyTagsExpressionMutationSoxS has R93A and S101A mutations and dCas9-NG ha…PromoterBBa_J23107 and Sp.pCas9Available sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYFP-ErbB2ΔC776
Plasmid#66947PurposeMammalian expression of rat ErbB2 ΔC776 tagged with YFPDepositorInsertErbB2 (Erbb2 Rat)
UseTags6xHis, V5 Tag, and YFPExpressionMammalianMutationDeletion of C776 (see depositor comment below on …PromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
ICAM5-bio-His
Plasmid#51759PurposeExpresses full-length Intercellular adhesion molecule 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertICAM5 (ICAM5 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
APOH-bio-His
Plasmid#52177PurposeExpresses full-length Beta-2-glycoprotein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertAPOH (APOH Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
CD226-bio-His
Plasmid#51646PurposeExpresses full-length CD226 antigen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertCD226 (CD226 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 hCortKD-Flcortmut3YD EGFP
Plasmid#187264PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsEGFPExpressionMutation3YD (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pRSV-CaMKIV(1-313)
Plasmid#45063PurposeExpression of constit active CaMKIVDepositorInsertCam Kinase IV(1-313) (Camk4 Mouse)
UseTagsExpressionMammalianMutationtruncated at amino acid 313*PromoterRSVAvailable sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-FKBP-LRRK2
Plasmid#193584PurposeExpresses human full-length LRRK2 with FKBP domain and 3xFLAG situated at the N-terminusDepositorInsertLeucine-rich repeat kinase 2 (LRRK2 Human)
UseTags3xFLAG and FKBPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
ECE1-His-bio
Plasmid#53361PurposeExpresses full-length Endothelin converting enzyme 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertECE1 (ECE1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 mCherry-hCortKD-Flcortmut3YD
Plasmid#187266PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmCherryExpressionMutation3YD (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 hCortKD-Flcortmut3YF mCherry
Plasmid#187265PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmCherryExpressionMutation3YF (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIE-hα2M-H6
Plasmid#128492PurposeExpresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.DepositorInsertA2M (A2M Human)
UseTagsHis tag (6x)ExpressionInsectMutationPromoterIE1Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only