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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,824 results for: nls

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Showing: 4701 - 4720 of 18824 results

  4. All_in_one_CRISPR/Cas9_LacZ

    Plasmid
    #74293
    Purpose
    "All-in-one" CRISPR/Cas9 (wt) plasmid for cloning of custom gRNA with blue/white screening
    Depositor
    Lynne Postovit
    Publication
    Findlay et al PLoS One. 2016 Apr 18;11(4):e0153901. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    LacZ-alpha
    Cas9
    mCherry
    Use
    CRISPR
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    Lac promoter, SV40, and T7 promoter
    Available since
    May 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    74293-plasmid-map-sequence-id-140421

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  5. pGfaABC1D-nLac

    Plasmid
    #53131
    Purpose
    expression of lacZ driven by human GfaABC1D promoter
    Depositor
    Michael Brenner
    Publication
    Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    contains ABC1D regions of Gfa2 promoter, -1757 to…
    Promoter
    human Gfa2
    Available since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    53131-plasmid-map-sequence-id-263310

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  6. pWPT-/GCCACC-mEGFP-IRES-mCherry

    Plasmid
    #49235
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49235-plasmid-map-sequence-id-376544

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  7. hIRE1a.pcD

    Plasmid
    #21892
    Depositor
    Randal Kaufman
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IRE1a (ERN1 Human)
    Expression
    Mammalian
    Available since
    Sept. 10, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    21892-plasmid-map-sequence-id-10067

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  8. pHS1861

    Plasmid
    #205974
    Purpose
    FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)
    Depositor
    Feng Zhang
    Publication
    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas8-HNH
    Tags
    FLAG-SV40 NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    205974-plasmid-map-sequence-id-415368

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  9. CRY-tTA (B702)

    Plasmid
    #92032
    Purpose
    Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator
    Depositor
    Chandra Tucker
    Publication
    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRY2 (CRY2 Mustard Weed)
    Tags
    tTA2 (TetR fused to VP64)
    Expression
    Mammalian
    Mutation
    NLS on CRY2 is mutated
    Promoter
    CMV
    Available since
    July 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    92032-plasmid-map-sequence-id-183915

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  11. GWF297

    Plasmid
    #133612
    Purpose
    TET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFP
    Depositor
    Dustin Maly
    Publication
    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP
    Expression
    Mammalian
    Available since
    Oct. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    133612-plasmid-map-sequence-id-261577

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  13. pCW-Tet-Cas9-BFP-PGK-Blast

    Plasmid
    #196720
    Purpose
    Tet-inducible expression of Cas9-T2A-TagBFP
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-T2A-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    3xFLAG-SV40NLS and Nucleoplasmin NLS
    Promoter
    TRE, PGK
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196720-plasmid-map-sequence-id-389756

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  15. pSLQ10875

    Plasmid
    #183963
    Purpose
    CBA-hyperdCas12a-HA-KRAB-mCherry
    Depositor
    Stanley Qi
    Publication
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyperdCas12a
    Use
    CRISPR
    Tags
    HA, KRAB, and mCherry
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CBA
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    183963-plasmid-map-sequence-id-363118

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  17. pLSB-KAN

    Plasmid
    #166700
    Purpose
    Combined sgRNA/Cas9 pLSB vector with kanMX6 (G418) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Publication
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA Ser promoter
    Available since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    166700-plasmid-map-sequence-id-329224

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Showing: 4701 - 4720 of 18824 results