We narrowed to 16,622 results for: puromycin

1. pME-puro-Venus-FLAG-CD59

   Plasmid

   #50377

   Purpose

   Express Venus and FLAG-tagged human CD59, a GPI-AP in mammalian cells

   Depositor

   Reika Watanabe

   Article

   Rivier et al Traffic. 2010 Aug;11(8):1017-33. doi: 10

   Insert

   Venus
   

   Expression

   Mammalian

   Promoter

   SR alpha

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pMSCV mLST8 myc (aka: GbL-myc)

   Plasmid

   #12580

   Depositor

   David Sabatini

   Article

   Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

   Insert

   mLST8 (MLST8 Human)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pSB-CMV-MCS-puro PM-mKate2-P2A-GEM-LTB4mut

   Plasmid

   #202644

   Purpose

   Expresses the LTB4-insensitive control sensor GEM-LTB4mut along with the plasma membrane targeted mKate2 tag in mammalian cells

   Depositor

   Balázs Enyedi

   Article

   Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

   Insert

   PM-mKate2-P2A-GEM-LTB4mut
   

   Tags

   PM-mKate2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-CALPAIN-7-mCherry

   Plasmid

   #180652

   Purpose

   Lentiviral expression vector for CALPAIN-7. Used for generating cell lines. Has C-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-50.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CALPAIN-7
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant to: GCACCCAUACCUUUACAUU

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-Puro-TDP-43-A382T

   Plasmid

   #133756

   Purpose

   Lentiviral expression of human TDP-43 A382T disease mutant in mammalian cells.

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Lentiviral

   Mutation

   Changed alanine 382 to threonine

   Promoter

   CMV

   Available Since

   Dec. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3xFlag-HaloTag-SUZ12 HDR - pDY082

   Plasmid

   #171110

   Purpose

   Homology directed repair plasmid for generating HaloTag-SUZ12 cells

   Depositor

   Thomas Cech

   Article

   Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

   Insert

   SUZ12 (SUZ12 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQCXIP-GFP-LmnB1

   Plasmid

   #164249

   Purpose

   retroviral plasmid for GFP-LmnB1

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   LMNB1 (LMNB1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

   Plasmid

   #70658

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pLKO.1P shSHMT2

   Plasmid

   #106319

   Purpose

   Express shRNA targeting SHMT2

   Depositor

   Richard Possemato

   Article

   Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

   Insert

   shRNA targeting SHMT2
   

   Use

   Lentiviral and RNAi

   Available Since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiGuide-RRBP1-1

    Plasmid

    #92156

    Purpose

    CRISPR guide RNA targeting human RRBP1

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 sgRNA-1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPE3-pPuro

    Plasmid

    #215722

    Purpose

    Cloning vector for Uni-Vector prime editing system

    Depositor

    Kou-Juey Wu

    Article

    Huang et al Biotechnol J. 2024 Feb;19(2):e2300353. d

    Insert

    PE2
    

    Use

    Unspecified

    Promoter

    EFS promoter

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDECKO_Malat1_C

    Plasmid

    #72622

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Expression

    Mammalian

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH_SBP-mCherry-GPI

    Plasmid

    #65299

    Purpose

    synchronize trafficking of mCherry-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Use

    Lentiviral

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMP-RING1_2

    Plasmid

    #36356

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    RING1 (RING1 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pMSCV-puro-Flag-mMcl-1 KR

    Plasmid

    #32983

    Depositor

    Joseph Opferman

    Article

    Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

    Insert

    Mcl-1 (Mcl1 Mouse)
    

    Use

    Retroviral

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    All Lys to Arg

    Promoter

    LTR

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

16. JN302: pMVP/SB/Puro-DEST

    Plasmid

    #121865

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Ppst93-ANME2bHR1 MCR-NG

    Plasmid

    #192774

    Purpose

    Expresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Available Since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. Ppst93-ANME1G37 MCR-NG

    Plasmid

    #192773

    Purpose

    Expresses NG-tagged ANME1G37 MCR in Methanococcus maripaludis

    Depositor

    William B. Whitman

    Article

    Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

    Insert

    Methyl coenzyme M reductase
    

    Use

    Synthetic Biology; Heterologous expression in met…

    Tags

    Flag-Strep2

    Available Since

    Dec. 7, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pMRXIP Lamp1-Venus

    Plasmid

    #89937

    Purpose

    Expresses Lamp1 tagged with Venus in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

    Insert

    rat Lamp1 (Lamp1 Rat)
    

    Use

    Retroviral

    Tags

    Venus

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDM002-sgRNA-lenti-ZeoR

    Plasmid

    #200060

    Purpose

    Lentiviral vector for expression of sgRNA with mCherry, zeocin resistance, BlpI + BstXI cloning sites

    Depositor

    Britt Glaunsinger

    Article

    McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

    Insert

    Bleomycin resistance, mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    mU6

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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