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Kindlin

Plasmid

#186135

Purpose

For expression of recombinant kindlin in bacteria

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

FERMT2 (FERMT2 Human)

Tags

His6-sumo

Expression

Bacterial

Promoter

T7

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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p130Cas Y15F

Plasmid

#186154

Purpose

For expressing recombinant p130Cas in insect cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

BCAR1 (Bcar1 Rat)

Tags

His6 (Tev)

Expression

Insect

Mutation

15Y residues mutated to Alanine

Promoter

polyhedrin

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Myc-DNMT3B2

Plasmid

#36942

Depositor

Arthur Riggs

Article

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Insert

DNMT3B2 (DNMT3B Human)

Tags

Myc

Expression

Mammalian

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-mNeonGreen-LEHD-NanoLuc

Plasmid

#98289

Purpose

caspase-9 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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p3xFlag-Dam

Plasmid

#121909

Purpose

Expresses E. coli Dam methyltransferase containing an N-ternimal 3xFlag tag

Depositor

Douglas Feldman

Article

Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

Insert

Dam human codon optimized (dam E. coli)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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MRAS

Plasmid

#55661

Purpose

expression of mouse MRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

MRAS (Mras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
ii-Str_SBP-mCherry-GPI

Plasmid

#65297

Purpose

synchronize trafficking of mCherry-GPI from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

GPI anchor

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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Rac1

Plasmid

#55692

Purpose

Expression of mouse Rac1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

Rac1 (Rac1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
p130Cas

Plasmid

#186131

Purpose

For expression of recombinant p130Cas in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

BCAR1 (Bcar1 Rat)

Tags

His6

Expression

Insect

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pRSETb-ffDronpa

Plasmid

#78182

Purpose

Expresses ffDronpa E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

ffDronpa

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-VEGFR2-225

Plasmid

#21306

Depositor

Donald Ingber

Article

Mammoto et al Nature. 2009 Feb 26. 457(7233):1103-8.

Insert

VEGFR2 promoter (KDR Human)

Use

Luciferase

Available Since

May 29, 2009

Availability

Academic Institutions and Nonprofits only
pBEG R2-L3 #BGV193

Plasmid

#48952

Purpose

For inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Type

Empty backbone

Use

Gateway cloning vector

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
SG3ΔENV CA M66I

Plasmid

#149687

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Capsid

Use

Lentiviral and Retroviral

Expression

Mammalian

Mutation

Changed Capsid residue Met 66 to Iso (CA M66I)

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pMR-A-6

Plasmid

#138520

Purpose

P3d

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-VMA1 chr I

Plasmid

#41896

Purpose

Yeast expression of VMA1.

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

VMA1 (VMA1 Budding Yeast)

Expression

Yeast

Mutation

Contains silent mutation at 342A-G and L87S mutat…

Promoter

GPD

Available Since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

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YCplac33-NAT

Plasmid

#140594

Purpose

Expression in S. cerevisiae

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-150

Plasmid

#64235

Purpose

Expresses miR-150 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR150 (MIR150 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLV U6-sgRNA UbC-tdTomato

Plasmid

#106949

Purpose

sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

sgRNA BsmBI/Esp3I cloning site

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pAXY0006

Plasmid

#205575

Purpose

Glucos(1-231)-NpuDnaE(N)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Inserts

Glucosyltransferase and NpuDnaE(N)_Arabidopsis-codon-OP
CYP76AD1-P2A-DODA

Expression

Plant

Promoter

35S

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG SMG1 T3550A/S3556A

Plasmid

#199574

Purpose

Expresses FLAG-tagged mutated SMG1

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

T3550A and S3556A

Promoter

CMV

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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