We narrowed to 11,585 results for: VARS

1. CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

   Plasmid

   #169743

   Purpose

   CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   mScarlet-I
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pCMV-tetO2

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LV_EF1a_RalA(G23V,full)-P2A-Hygro_Barcode

   Plasmid

   #170249

   Purpose

   Barcoded lentiviral vector to express RalA(G23V, full) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   RALA (RALA Human)
   

   Use

   Lentiviral

   Mutation

   G23V

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGMC00008

   Plasmid

   #166724

   Purpose

   sgRNA cloning backbone

   Depositor

   Raj Chari

   Article

   Choi et al Nat Commun. 2020 Jun 1;11(1):2718. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Equalizer-M plasmid with onboard mCherry cassette

   Plasmid

   #169734

   Purpose

   Equalizer-M plasmid that encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV-tetO2

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMS5: pHelper(AvCAST)_entry_ΔTniQ

   Plasmid

   #168138

   Purpose

   Inducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB and TnsC)
   AvTnsD
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS4: pHelper(AvCAST)_entry

   Plasmid

   #168137

   Purpose

   Inducible expression of AvCAST proteins. Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   AvTnsD
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMS2: pHelper(AvCAST)_AvPSP1_ΔTniQ

   Plasmid

   #168134

   Purpose

   Inducible expression of AvCAST proteins (except TniQ) with crRNA targeting AvPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array (with spacer for AvPSP1)
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB and TnsC)
   AvTnsD
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-GFAP104-ChromeT-mCherry

   Plasmid

   #123126

   Purpose

   AAV-mediated expression of ChromeT-mCherry under the GFAP promoter.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeT-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

   Promoter

   GFAP104

   Available Since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCFJ2308 - [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

   Plasmid

   #159852

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - mNeon(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEMS2267

    Plasmid

    #158392

    Purpose

    High Copy number plasmid containing Ple341 (PCP2 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple341 (PCP2 Human)
    

    Use

    Unspecified

    Promoter

    Ple341

    Available Since

    Feb. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pEMS2279

    Plasmid

    #158399

    Purpose

    High Copy number plasmid containing Ple349 (PDE6H MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

    Insert

    Ple349 (PDE6H Human)
    

    Use

    Unspecified

    Promoter

    Ple349

    Available Since

    Feb. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]

    Plasmid

    #159838

    Purpose

    Three-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arrays

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO-Cre sgUroD v4

    Plasmid

    #158036

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroD

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    UroD sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pLKO-Cre sgItgb5 v3

    Plasmid

    #158038

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Itgb5 sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLKO-Cre sgRipk4 v3

    Plasmid

    #158035

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Ripk4 sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pFA0057

    Plasmid

    #131784

    Purpose

    Guide RNA (gGFP) and Cas9 expression plasmid for cleaving pFA6 series GFP C-terminal tagging cassettes, including GFP-His3MX6. Used to perform CRISPR-Swap of alleles.

    Depositor

    Frank Albert

    Article

    Lutz et al PLoS Genet. 2019 Nov 18;15(11):e1008375.

    Insert

    20mer GFP cassette guide (gGFP) and 5' sgRNA
    

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Syn-ChromeT-GFP

    Plasmid

    #123316

    Purpose

    AAV-mediated expression of ChromeT-GFP under the Syn promoter.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Channelrhodopsin-2 (ChR2) with mutations A71S/ E9…

    Promoter

    Syn

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPKm- 227

    Plasmid

    #90498

    Purpose

    pcDNA3 - CMVmin - VPR - PIF3, expresses VPR transactivator domain and PIF3, under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    VP64-PIF3
    

    Expression

    Mammalian

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPKm-113

    Plasmid

    #90495

    Purpose

    pcDNA3 -pCMV - MTAD - PIF6, expresses the Phytochrome interacting factor 6 (PIF6) and MTAD, under CMV promoter

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    MTAD-PIF6
    

    Expression

    Mammalian

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHH0103_BZRAP1-2/3

    Plasmid

    #91551

    Purpose

    Protein expression and purification of human SH3 domain construct BZRAP1-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BZRAP1-2/3 (TSPOAP1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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