We narrowed to 9,611 results for: cre encoding plasmid

1. pZBED9.1.1-gDNA

   Plasmid

   #175854

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   ZBED9-Nickase1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZBED9.1.2-gDNA

   Plasmid

   #175853

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   ZBED9-Nickase2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDX4.1.1-gDNA

   Plasmid

   #175852

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   CDX4-Nickase1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDX4.1.2-gDNA

   Plasmid

   #175851

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   CDX4-Nickase2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZNF468-donor

   Plasmid

   #176350

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   ZNF468 (ZNF468 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. ZMAT4.1.1-gDNA

   Plasmid

   #175871

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   ZMAT4-Nickase1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ZMAT4.1.2-gDNA

   Plasmid

   #175872

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   ZMAT4-Nickase2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HEY2.1.1-gDNA

   Plasmid

   #175873

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   HEY2-Nickase1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HEY2.1.2-gDNA

   Plasmid

   #175874

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   HEY2-Nickase2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. OVOL2.2.1-gDNA

    Plasmid

    #175876

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    OVOL2-Nickase1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. OVOL2.2.2-gDNA

    Plasmid

    #175877

    Purpose

    CRISPR/Cas9 plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    OVOL2-Nicakse2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

    Plasmid

    #169193

    Purpose

    To express SARS-CoV-2 Papain-like protease in E. coli

    Depositor

    John Diffley

    Article

    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

    Insert

    14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK27Sumo_His-SUMO-nsp10-nsp14 fusion (SARS-CoV-2)

    Plasmid

    #169161

    Purpose

    To express nsp10-nsp14 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    14His-SUMO-nsp10-GGSGGS-nsp14 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

    Plasmid

    #169160

    Purpose

    To express nsp14-nsp10 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid

    #169184

    Purpose

    Baculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-His6-3xFlag/nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    His6-3xFlag (nsp12 C-terminus) and nsp7-nsp8 fusi…

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169164

    Purpose

    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    6His-3xFlag (nsp10 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTSAR3.4t

    Plasmid

    #210256

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    mCherry
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    Feb. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TU#1806_CRISPR_unc-73_exon21

    Plasmid

    #82360

    Purpose

    to create unc-73E null allele

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Cas9 and sgRNA against unc-73 exon21 (unc-73 Nematode)
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTSAR3.7t

    Plasmid

    #210259

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    e2Crimson
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTSAR3.3t

    Plasmid

    #210255

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    DsRed
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge