We narrowed to 10,362 results for: Coli
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Plasmid#249631PurposeClone MBP polypeptide in the N-terminal of a protein to be expressed using pET28g backbone, using fusion sites 1 and 3DepositorInsertN-terminal tag with MBP polypeptide
ExpressionBacterialMutationSilent mutation on Gly-20 to remove a BsaI recogn…Available SinceApril 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
pET-Eco7-RT-PtuA-PtuB
Plasmid#252300PurposeExpresses Retron-Eco7 proteins in bacterial cells.DepositorInsertRetron-Eco7 proteins
ExpressionBacterialMutationWTAvailable SinceMarch 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
p-ArsRBS2-cre[LVA]-loxPP-syfp2
Plasmid#252621PurposeCre-recombinase based arsenite biosensor. ArsR promoter regulates cre expression, which excises two transcriptional terminators leading to cell fluorescence.DepositorInsertParsR-arsR-cre[LVA] + PrpsL-loxP-TT-loxP-syfp2
Tagscre[LVA]ExpressionBacterialPromoterParsRAvailable SinceMarch 30, 2026AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1-Eco7-ncRNA
Plasmid#252301PurposeExpresses the Retron-Eco7 non-coding RNA in bacteria cells.DepositorInsertRetron-Eco7 non-coding RNA
ExpressionBacterialMutationWTAvailable SinceMarch 20, 2026AvailabilityAcademic Institutions and Nonprofits only -
p-cymR-cre[LVA]-loxPP-eyfp
Plasmid#252620PurposeCumate-inducible Cre recombinase; cumate activates P_cymRC to express cre[LVA], which excises loxP-flanked transcriptional terminators and activates eyfp. Use with Marionette Sensor Collection strainsDepositorInsertcymRAM + PcymRC-cre[LVA] + PW4-loxP-TT-loxP-eyfp
Tagscre[LVA]ExpressionBacterialPromoterPcymRCAvailable SinceMarch 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pA_higher-Opto_N449S
Plasmid#253258PurposeOpto-CheY N449S expressed using the proA promoter and wild type T7 RBSDepositorInsertOpto-CheY with N449S mutation
Tagswild type T7 RBSExpressionBacterialAvailable SinceMarch 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pA-Opto_N449S
Plasmid#253256PurposeOpto-CheY N449S expressed using the proA promoter and T7 RBS variant GAAGGAGgTDepositorInsertOpto-CheY with N449S mutation
TagsT7 RBS variant GAAGGAGgTExpressionBacterialAvailable SinceMarch 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pA-Opto_V416T
Plasmid#253257PurposeOpto-CheY V416T expressed using the proA promoter and T7 RBS variant GAAGGAGgTDepositorInsertOpto-CheY with V416T mutation
ExpressionBacterialMutationT7 RBS variant GAAGGAGgTAvailable SinceMarch 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Eco7-ncRNA-RT-PtuA-PtuB
Plasmid#252299PurposeExpresses the Retron-Eco7 operon in bacterial cells.DepositorInsertRetron-Eco7 operon
ExpressionBacterialMutationWTAvailable SinceMarch 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pBA1583_ECOR31HamA(E138AK140A)B_CoPurification
Plasmid#223372PurposeT7 protein expression of MBP-His-TEV-ECOR31 Hachiman mutant (HamA E138A, K140A)DepositorInsertMBP-His-TEV-ECOR31 Hachiman mutant (HamA E138A, K140A)
TagsMBP-His-TEVExpressionBacterialMutationHamA E138A, K140AAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pNP2085
Plasmid#222731PurposeRep101 Ts - IS621 WT Donor (22bp) with Bba_R0040-KanR Cargo and Rep101 Temp SensitiveDepositorInsertIS621 Donor
ExpressionBacterialAvailable SinceDec. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBA1751
Plasmid#246066PurposeBacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pJex promoter; SC101 oriDepositorInsertPanoptes locus
ExpressionBacterialPromoterpJEX promoterAvailable SinceDec. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBA1729
Plasmid#246063PurposeBacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a oriDepositorInsertPanoptes locus
ExpressionBacterialPromoterpTet promoterAvailable SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBN415
Plasmid#217920Purposeexpress E. coli E69 (O9a:K30) Wzc with N711Y mutationDepositorInsertE. coli E69 (O9a:K30) Wzc with N711Y mutation
Tagshexa-histidine tagExpressionBacterialMutationN711YAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF18
Plasmid#229456PurposeExpresses IraD (aa17-130) with His-tagDepositorInsertIraD (aa 17-130 only)
Tags6xHisExpressionBacterialMutationaa 17-130 onlyPromoterT7Available SinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
SBC015898
Plasmid#226285PurposeExpresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.DepositorInsertsConiferyl alcohol acyltransferase
Eugenol synthase 1
Eugenol O-methyltransferase
ExpressionBacterialAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-Tir
Plasmid#221326PurposeDox-inducible expression of Tir in mammalian cells by retroviral transductionDepositorInsertTir
UseRetroviralTagsHN-FLAGPromoterTRE3GVAvailable SinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWR88
Plasmid#203422Purposeempty BLINCAR plasmid for payload delivery, no payload, no genomic targetDepositorInsertsCAR copy 1
P(TEF1)/coKanMX/T(ACT1) + P(TDH3)/coCBG/T(ADH2)
CAR copy 2
ExpressionYeastAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMBA337
Plasmid#214750PurposeMedium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validationDepositorInsertBBa_J23119-RiboJ-RBS(21992)-gfpmut3-RBS(4533)-specR
ExpressionBacterialAvailable SinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-TrxF
Plasmid#207454PurposeE. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tagDepositorInsertThioredoxin F
Tags6xHisExpressionBacterialMutationCodon optimized for E coli expressionPromoterT7Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only