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pBEST-p70a-UTR1-Nterm 6xHis PL-T500

Plasmid

#92314

Purpose

Expression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

B1 domain of protein L (PL)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pBB0323-TEV-His12

Plasmid

#137039

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66700.1

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBEST-p70b-UTR1-deGFP-6xHis-T500

Plasmid

#92222

Purpose

Expression of deGFP using the p70b promoter (weak version of the p70a promoter)

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70b (p70a promoter with one mutation)

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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p1585588

Plasmid

#62620

Purpose

ePathBrick pETM6 vector encoding CsF3H, CsDFR, and DuLAR (1:3:3 Ratio)

Depositor

Mattheos Koffas

Article

Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

Inserts

flavaone 3B-hydroxylase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
dihydroflavonol 4-reductase
dihydroflavonol 4-reductase
leucoanthocyanidin reductase
leucoanthocyanidin reductase

Use

Synthetic Biology

Tags

GBD, PDZ, and SH3

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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WT MetNI in pBAD

Plasmid

#118254

Purpose

Wild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity Assay

Depositor

Douglas Rees

Article

Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

Inserts

MetN (metN Escherichia coli)
MetI (metI Escherichia coli)

Tags

10x His Tag and Enterokinase Cleavage Site

Expression

Bacterial

Promoter

Arabinose Promoter and None

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pMM549

Plasmid

#112531

Purpose

pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA

Expression

Bacterial

Promoter

J23107 and ProD

Available Since

Feb. 15, 2020

Availability

Academic Institutions and Nonprofits only

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R0063 (pLuxR-pR)_AB

Plasmid

#66012

Purpose

MoClo Basic Part: Controllable promoter - pLuxR(pR) - luxR regulated (right promoter, weak constitutive down regulated by LuxR, C0062 in combination with homoserine lactone (HSL)) [A:R0063:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-p70a-UTR1-Nterm 6xHis mRFP-T500

Plasmid

#92316

Purpose

Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR1

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

Optimized NTerm 6xHis tagged monomeric RFP (mRFP)

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pZS1[FruR-T]

Plasmid

#60752

Purpose

Contains PIq driving expression FruR-T, the Fructose inducible chimera with the TAN DBD.

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Inserts

FruR-T
FruR-T

Use

Synthetic Biology

Expression

Bacterial

Mutation

Chimeric LacI/GalR repressor with the TAN DBD and…

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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hSRAP.HMG6.wt.Dlink.a1-236

Plasmid

#49813

Purpose

expression vector for MBP-SRAP(1-236) fusion

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein (SRA1 Human)

Tags

E. coli Maltose Binding Protein

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pBEL3016

Plasmid

#232528

Purpose

Expression plasmid of LetA(T402A) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetA T402A

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL3017

Plasmid

#232529

Purpose

Genetic complementation plasmid of LetA(PqiA-ZnR) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetA(PqiA-ZnRN/PqiA-ZnRC)

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL3158

Plasmid

#232530

Purpose

Genetic complementation plasmid of LetB(R63A) with LetA

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetB R63A

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL3159

Plasmid

#232531

Purpose

Genetic complementation plasmid of LetB(R63N) with LetA

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetB R63N

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL3160

Plasmid

#232532

Purpose

Genetic complementation plasmid of LetB(R63D) with LetA

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetB R63D

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2879

Plasmid

#232519

Purpose

Genetic complementation plasmid of LetA(ΔZnR-C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Expression

Bacterial

Mutation

LetA ΔZnR-C

Promoter

T7

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2913

Plasmid

#232523

Purpose

Expression plasmid of LetA(ΔZnR-N) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetA ΔZnR-N

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2914

Plasmid

#232524

Purpose

Expression plasmid of LetA(ΔZnR-C) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetA ΔZnR-C

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL2921

Plasmid

#232525

Purpose

Expression plasmid of LetA(S364A) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetA S364A

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBEL3014

Plasmid

#232526

Purpose

Expression plasmid of LetA(D181A) with LetB

Depositor

Gira Bhabha, Damian Ekiert

Article

Santarossa et al bioRxiv 2025.03.21.644421

Insert

YebST (letA, letB E. coli)

Tags

6xHis2xQH-TEV

Expression

Bacterial

Mutation

LetA D181A

Promoter

araBAD

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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