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pGH335_MS2-AID*Δ-Hygro

Plasmid

#85406

Purpose

lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID*Δ and Hygromycin resistance

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCI-SEP_NR2A

Plasmid

#23997

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

NR2A (Grin2a Rat)

Tags

SEP

Expression

Mammalian

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pU6-GG-gRNA

Plasmid

#226860

Purpose

Human gRNA expression vector backbone with BsmBI Golden Gate site

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

Sp-gRNA

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pNHEJ-recX-sacB

Plasmid

#158718

Purpose

CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recX.

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

recX

Expression

Bacterial

Promoter

Pmyc

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG>nls-Cas9-nls-2A-Citrine-EcoRI/NotI

Plasmid

#169097

Purpose

Cas9 and Citrine expression in transfected cells

Depositor

Marianne Bronner

Article

Gandhi et al Development. 2021 Mar 9. pii: dev.193565

Insert

Cas9-2A-Citrine

Expression

Mammalian

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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piggyBac-ABE8.20-NG

Plasmid

#226866

Purpose

Expresses dox-inducible ABE8.20-NG for mammalian cells. piggyBac vector

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

ABE8.20-NG

Use

Lentiviral

Promoter

Tet-On-TRE3G

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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ABE8e-NG

Plasmid

#226856

Purpose

Expresses ABE8e-NG base editor in mammalian cells with eGFP marker

Depositor

Alexis Komor

Article

Osgood et al Methods Enzymol. 2025;712:351-404. doi:

Insert

ABE8e-NG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1

Plasmid

#171614

Purpose

AsCas12f1-based Human cell genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1 and sgRNA_v1

Expression

Mammalian

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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HH-gRNA-HDV-Shuttle-Vector

Plasmid

#169098

Purpose

Backbone plasmid to generate HH-gRNA-HDV segment

Depositor

Marianne Bronner

Article

Gandhi et al Development. 2021 Mar 9. pii: dev.193565

Insert

HH-gRNAbackbone-HDV

Expression

Mammalian

Promoter

NA

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLX304-Lantern-HaloTag-NES

Plasmid

#250919

Purpose

Expresses Lantern in mammalian cytosol

Depositor

Peng Zou

Article

Fang et al Research Square 2025 [Preprint]. https:/

Insert

Lantern

Use

Lentiviral

Tags

HaloTag-NES and V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-HA-hM4D(Gi)

Plasmid

#45548

Purpose

Expresses hM4D (Gi) neuronal inhibitor

Depositor

Bryan Roth

Article

Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

Insert

hM4D (Gi)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

Plasmid

#114726

Purpose

dCas9-VP160 expression in mammalian cells

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

dCas9-VP160

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pPP085

Plasmid

#158795

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-LKB1(WT)

Plasmid

#84639

Purpose

mCherry-tagged, wild-type LKB1

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

mCherry-LBK1 (STK11 Human, Synthetic)

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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Twin-Strep(R)-Tev-GSGS-Ubiquitin

Plasmid

#186803

Purpose

Codon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

Twin-Strep(R)-Tev-GSGS-Ubiquitin

Tags

GS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…

Expression

Bacterial

Mutation

Strep tag Lysine's mutated to Arginine

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HaloTag N58-(GGS)X6-Lifeact

Plasmid

#171436

Purpose

Expression of HaloTag N58-(GGS)X6-Lifeact in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

HaloTag N58-(GGS)X6-Lifeact

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pYC2085

Plasmid

#158721

Purpose

CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

Depositor

Yi-Cheng Sun

Article

Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

Insert

Sth1 sgRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG/GFP

Plasmid

#139980

Purpose

expresses GFP under the promoter of CAG. Transgene flanked by AAV2 ITR

Depositor

William Pu

Article

Wang et al Circ Res. 2020 Mar 9. doi: 10.1161/CIRCR

Insert

Green fluorescent protein

Use

AAV

Promoter

CAG

Available Since

April 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58

Plasmid

#171435

Purpose

Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells

Depositor

Yujie Sun

Article

Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

Insert

Lifeact-(GGS)X6-HaloTag C58

Expression

Mammalian

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Lentiviral-FKBP-cpGFP-Nb39

Plasmid

#191498

Purpose

Rapamycin-dependent opioid sensor for opioid receptors (component 2)

Depositor

Wenjing Wang

Article

Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

Insert

FKBP-cpGFP-Nb39

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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