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tubP-FRT>GAL80-6-FRT>

Plasmid

#63173

Purpose

ubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassette

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"Flp-Out" GAL80

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSF464

Plasmid

#167672

Purpose

Intermediate vector for four gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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Cas9 TLS1

Plasmid

#196977

Purpose

Expresses Cas9 fused with TLS1 mobility signal that confers graft mobility. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9 with TLS1 tag

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSIM24-GUS

Plasmid

#67882

Purpose

Plant gene expression vector pSIM24 containing the GUS (Beta-glucuronidase) reporter gene

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Insert

uidA

Expression

Plant

Mutation

T120A

Promoter

M24

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-ABEmax-BlastR

Plasmid

#152989

Purpose

A-to-G base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

ABE7.10-nSpCas9

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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HisTEV-cDAXX

Plasmid

#197012

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

DAXX

Tags

6His-TEV

Expression

Bacterial

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ131A2.0

Plasmid

#99884

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pBJ2

Plasmid

#167131

Purpose

A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Expression

Bacterial

Promoter

Kanamycin promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-DIO-mTurquoise2

Plasmid

#104059

Purpose

AAV expression of Cre-dependent reporter mTurquoise2

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

mTurquoise2

Use

AAV

Promoter

CAG

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pComb3XLambda

Plasmid

#63892

Purpose

Backbone pComb3X containing PCR template for human antibody lambda light chain constant region

Depositor

Carlos Barbas

Article

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Insert

human antibody lambda light chain (IGLL5 Human)

Use

Phage display

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCMU-ACTLr

Plasmid

#61193

Purpose

Fluorescent reporter for F-actin LifeAct-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LifeAct-mCherry

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-BirA-eCPX-AP

Plasmid

#121907

Purpose

Expresses BirA and eCPX-AP

Depositor

Per Svenningsen

Article

Granhoj et al Sci Rep. 2019 Mar 11;9(1):4118. doi: 10.

Inserts

BirA (birA Synthetic)
Enhanced Circularly Permuted Outer Membrane Protein X

Tags

6xHis

Expression

Bacterial

Promoter

T7 and pBAD promoter

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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LentiV2-EF1α-nCas9/RT

Plasmid

#210188

Purpose

Expression of nCas9/RT-P2A-Puro

Depositor

Yin Shen

Article

Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

Insert

nCas9/RT-P2A-Puro

Use

Lentiviral

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCMU-NUCr

Plasmid

#61168

Purpose

Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

NLS-mCherry-GUS

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pBJ6

Plasmid

#167135

Purpose

A gentamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pkan promoter

Depositor

Akira Mine

Article

Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

Insert

luxCDABE

Use

Luciferase

Expression

Bacterial

Promoter

Kanamycin promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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SaKKH-ABEmax

Plasmid

#119815

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSaCas9 (KKH)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pRDA_078

Plasmid

#158582

Purpose

Lentiviral expression of BE4max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pME_G_E_004_UAV_mScarlet-I

Plasmid

#235974

Purpose

Universal acceptor vector with mScarlet-I placeholder for lvl0 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

UAV_mScar

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-T2A-moxMaple3

Plasmid

#120875

Purpose

Mammalian expression of mCherry and moxMaple3

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Inserts

mCherry
moxMaple3

Expression

Mammalian

Mutation

from mMaple3, moxMaple3 was engineered by replaci…

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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EMTB-mScarletI

Plasmid

#137801

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Ensconsin microtubule binding domain

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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