We narrowed to 10,068 results for: actin

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PB-CAG::Cytbow

Plasmid

#158995

Purpose

Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Cytbow

Tags

The default expressed EBFP2 is fused to H2B

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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PB-CAG::Cytbow

Plasmid

#158995

Purpose

Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

Depositor

Jean Livet

Article

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Cytbow

Tags

The default expressed EBFP2 is fused to H2B

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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LiOn-CAG∞RFP

Plasmid

#154016

Purpose

Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

mRFP1

Expression

Mammalian

Mutation

H210P

Promoter

CAG

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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LiOn-CAG∞RFP

Plasmid

#154016

Purpose

Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase

Depositor

Jean Livet

Article

Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

Insert

mRFP1

Expression

Mammalian

Mutation

H210P

Promoter

CAG

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-SponGee

Plasmid

#134775

Purpose

mammalian expression of SponGee, a buffer of cGMP that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

SponGee

Tags

FLAG and mRFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCX-SponGee

Plasmid

#134775

Purpose

mammalian expression of SponGee, a buffer of cGMP that prevents downstream signaling

Depositor

Xavier Nicol

Article

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

SponGee

Tags

FLAG and mRFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Target-AID (pRZ762)

Plasmid

#131300

Purpose

CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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Target-AID (pRZ762)

Plasmid

#131300

Purpose

CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

Insert

NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pR26-CAG-H2B-miRFP703

Plasmid

#112853

Purpose

Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

H2B-miRFP703

Expression

Mammalian

Promoter

CAG

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pR26-CAG-H2B-miRFP703

Plasmid

#112853

Purpose

Donor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell lines

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

H2B-miRFP703

Expression

Mammalian

Promoter

CAG

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.A184V

Plasmid

#106199

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-iGluSnFR.A184V

Plasmid

#106199

Purpose

Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-iGluSnFR.A184V

Use

AAV

Mutation

GltI: A184V

Promoter

CAG

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Archon1-KGC-EGFP-ER2

Plasmid

#108420

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Archon1-KGC-EGFP-ER2

Plasmid

#108420

Purpose

AAV production plasmid encoding for Archon1 fluorescent voltage reporter

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

Archon1-KGC-EGFP-ER2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-Venus-iGluSnFR.A184S

Plasmid

#106201

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.SF-Venus-iGluSnFR.A184S

Plasmid

#106201

Purpose

Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Insert

SF-Venus-iGluSnFR.A184S

Use

AAV

Mutation

SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG vGlut1 GCaMP5G

Plasmid

#105008

Purpose

Presynaptic calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

SLC17A7

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG vGlut1 GCaMP5G

Plasmid

#105008

Purpose

Presynaptic calcium sensor

Depositor

Franck Polleux

Article

Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

Insert

SLC17A7

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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CAG-SaCas9-WPRE

Plasmid

#89996

Purpose

Expresses high levels of WT SaCas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SaCas9 (NEWENTRY Staphylococcus aureus)

Tags

3xHA, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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CAG-SaCas9-WPRE

Plasmid

#89996

Purpose

Expresses high levels of WT SaCas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SaCas9 (NEWENTRY Staphylococcus aureus)

Tags

3xHA, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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