We narrowed to 48,883 results for: des.1

1. pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

   Plasmid

   #159874

   Purpose

   Three-fragment Gateway compatible protein tag for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

   Plasmid

   #78306

   Purpose

   Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-nlsLexA::GADfluw-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. APL0038 Ac5-mCherry-SGc(stem-1(3xMS2))cSG-EGFP

   Plasmid

   #233410

   Purpose

   AC5 promoter driven LIDAR reporter RNA for drosophila cell expression (mCherry as marker, EGFP as output)

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   mCherry-SGc(stem-1(3xMS2))cSG-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Ac5

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

   Plasmid

   #99696

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

   Depositor

   George Church

   Insert

   dCas9 and gRNA targeting Afp (Afp Synthetic)
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-6P-1 Caenorhabditis elegans CNNM2 CBS-pair domain

   Plasmid

   #166433

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM2 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM2 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX-6P-1 Caenorhabditis elegans CNNM5 CBS-pair domain

   Plasmid

   #166434

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM5 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM5 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-6P-1 Caenorhabditis elegans CNNM1 CBS-pair domain

   Plasmid

   #166508

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM1 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM1 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-6P-1 Caenorhabditis elegans CNNM3 CBS-pair domain

   Plasmid

   #166510

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM3 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM3 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-6P-1 Caenorhabditis elegans CNNM4 CBS-pair domain

   Plasmid

   #166511

   Purpose

   Express pGEX-6P-1 Caenorhabditis elegans CNNM4 CBS-pair domain

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Caenorhabditis elegans CNNM4 CBS-pair domain
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac, LacI, Amp

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)

    Plasmid

    #238181

    Purpose

    Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Insert

    KLF5 (KLF5 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)

    Plasmid

    #238184

    Purpose

    Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al bioRxiv [Preprint]. 2024 Aug 3:2024.07.3

    Insert

    KLF5 (KLF5 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEF myc Gal cyclin T1 (1-726) (P#1026)

    Plasmid

    #14626

    Depositor

    Matija Peterlin

    Article

    Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

    Insert

    cyclin T1 (CCNT1 Human)
    

    Use

    Tags

    Gal and myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Plasmid

    #101218

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A and H698A

    Promoter

    T7

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75232

    Purpose

    CRISPR/Cas9 plasmid against human NFATc2

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against NFATc2 (NFATC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Plasmid

    #101220

    Purpose

    Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant

    Depositor

    Keith Joung, Jennifer Doudna

    Article

    Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

    Insert

    SpCas9 variant N692A/M694A/Q695A/H698A/Q926A
    

    Use

    Tags

    10x His, MBP, and TEV site

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A and Q926A

    Promoter

    T7

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

    Plasmid

    #200793

    Purpose

    Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

    Depositor

    Alison Twelvetrees

    Article

    Smith et al bioRxiv 2024.12.20.629623

    Insert

    KIF5A (Kif5a Mouse)
    

    Use

    Tags

    CLIPf and SNAPf, HisTag, FLAG

    Expression

    Mammalian

    Mutation

    1-914; Δ505-610

    Promoter

    CMV & T7

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLINK cyclin T1 (1-280) myc (K93D) (P#580)

    Plasmid

    #14611

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-280) K93D (CCNT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K93D. Deficient in binding to CDK9. Inactive in 3…

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid

    #138524

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    PRC2-binding G-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA4-Tet-on-vsv-CenpB 1-166-dCEN- Incenp-GFP

    Plasmid

    #108484

    Purpose

    inducible expression of vsv-CenpB-1-166-dCEN-INCENP-EGFP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    CenpB-dCEN-INCENP (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    CEN box replaced by CenpB 1-166

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CMV-GFP-pA U6 shRNA (Rat NPAS4 #1)

    Plasmid

    #233270

    Purpose

    To express GFP from a CMV promoter and to express and shRNA targeting Rat Npas4

    Depositor

    Jonathan Ploski, Glenn Schafe

    Article

    Ploski et al PLoS One. 2011;6(8):e23760. doi: 10.1371

    Insert

    GFP and shRNA targeting Rat NPAS4 (Npas4 Rat)
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge