We narrowed to 18,398 results for: nar;

1. pET28a_6HT-TBP6.7(P51A)

   Plasmid

   #112728

   Purpose

   Similar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(P51A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   P51A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a_6HT-TBP6.7(Q54A)

   Plasmid

   #112730

   Purpose

   Although not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(Q54A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   Q54A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LLhT-pHG165a V52A

   Plasmid

   #90050

   Purpose

   Constitutive expression of a chimeric LacI:TreR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhT/V52A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V52A

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. LLhA-pHG165a Q55A

   Plasmid

   #90052

   Purpose

   Constitutive expression of a chimeric LacI:AscG transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhA/Q55A
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q55A

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. LLhC-pHG165a Q55I

   Plasmid

   #90053

   Purpose

   Constitutive expression of a chimeric LacI:CytR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   LLhC/Q55I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Q55I

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tpa1(21-644)

   Plasmid

   #23140

   Depositor

   SeWon Suh

   Article

   Kim et al Nucleic Acids Res. 2010 Apr;38(6):2099-1

   Insert

   Tpa1 (TPA1 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-20

   Promoter

   Available since

   Sept. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pVE13300

   Plasmid

   #137715

   Purpose

   Expresses impLbCas12a in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   impLbCas12a
   

   Use

   Tags

   3xHA, NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. KP501: pMVP (L5-L4) eYFP-P2A

   Plasmid

   #121714

   Purpose

   pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   eYFP-P2A (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNPK-NLS-Vika

   Plasmid

   #79969

   Purpose

   expresses human codon-optimized Vika-recombinase in mammalian cells

   Depositor

   Frank Buchholz

   Article

   Karimova et al Nucleic Acids Res. 2013 Jan;41(2):e37. d

   Insert

   human codon-optimized Vika sequence, NLS tagged
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. psPAX2-D64V-NC-MS2

    Plasmid

    #122944

    Purpose

    MS2 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an MS2 aptamer into lentivirus-like particles

    Depositor

    Baisong Lu

    Article

    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

    Insert

    NC-MCP (MS2 coat protein) fusion protein in Gag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D64V

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. ires-eGFP(+HAs) donor-1

    Plasmid

    #87865

    Purpose

    Express eGFP reporter for HDR-induced knock-in

    Depositor

    Bo Feng

    Article

    He et al Nucleic Acids Res. 2016 May 19;44(9):e85

    Insert

    eGFP
    

    Use

    Homologous recombination for gapdh loci

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSP2-96-merTetO-EFS-BLaR

    Plasmid

    #118713

    Purpose

    Contains an optimized 96-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNEU-hMbPylRS-4xU6M15

    Plasmid

    #105830

    Purpose

    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells

    Depositor

    Irene Coin

    Article

    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464

    Inserts

    Humanized MbPylRS
    tRNAM15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    (Y271A, Y349F)MbPylRS

    Promoter

    CMV and U6

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1-FLAG

    Plasmid

    #60360

    Purpose

    Plasmid for cloning and expression of FLAG-GFP tagged proteins (C-terminal tag). Confers resistance to G418.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. psPAX2-D64V-NC-PP7

    Plasmid

    #122945

    Purpose

    PP7 coat protein-modified lentiviral packaging plasmid for packaging mRNA with an PP7 aptamer into lentivirus-like particles

    Depositor

    Baisong Lu

    Article

    Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

    Insert

    NC-PCP (PP7 coat protein) fusion protein in Gag
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D64V

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. HA-DDX3

    Plasmid

    #44975

    Depositor

    Robin Reed

    Article

    Lee et al Nucleic Acids Res. 2008 Aug;36(14):4708-

    Insert

    DDX3 (DDX3X Human)
    

    Use

    Tags

    HA and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSP2-48-merTetO-EFS-BLaR

    Plasmid

    #118712

    Purpose

    Contains an optimized 48-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. dCas9 plasmid

    Plasmid

    #100091

    Purpose

    dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFlag-NLS-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. LentiCRISPRv2 EGFP sgRNA1

    Plasmid

    #164932

    Purpose

    Expresses EGFP sgRNA1 in mammalian cells

    Depositor

    Ji Luo

    Article

    Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

    Insert

    sgRNA1 targeting Enhanced green fluorescent protein (verified for knockout)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti F9-TetR-EGFP-IRES-PuroR

    Plasmid

    #117049

    Purpose

    lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Insert

    F9-TetR-EGFP
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    F9 promoter

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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