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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,356 results for: Met

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Showing: 4741 - 4760 of 14356 results

  1. Venus1-Y705F/S727A-STAT3

    Plasmid
    #123176
    Purpose
    Expresses Y705F/S727A STAT3 double mutant fused to the 1-157 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Publication
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
    Tags
    Venus1 (aa 1-158) for bimolecular fluorescence co…
    Expression
    Mammalian
    Mutation
    Double Y705F and S727A substitutions
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123176-plasmid-map-sequence-id-238648

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  2. Venus2-Y705F/S727A-STAT3

    Plasmid
    #123177
    Purpose
    Expresses Y705F/S727A STAT3 double mutant fused to the 158-238 fragment of Venus in mammalian cells
    Depositor
    Federico Herrera
    Publication
    Letra-Vilela et al FASEB Bioadv. 2020 Jan 27;2(2):116-125.
    Insert
    STAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
    Tags
    Venus2 (aa 158-238) for bimolecular fluorescence …
    Expression
    Mammalian
    Mutation
    Double Y705F and S727A substitutions
    Available since
    Aug. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123177-plasmid-map-sequence-id-238660

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  4. pICE-EGFP-FLAG-Ku70siR-Mut6E

    Plasmid
    #46962
    Purpose
    For constitutive or doxycycline-inducible expression of mutant human Ku70 unable to bind DNA and resistant to siRNA. Confers resistance to puro. Use T-REx cells for doxycycline-inducible expression.
    Depositor
    Steve Jackson
    Publication
    Britton et al J Cell Biol. 2013 Jul 29.
    Insert
    Ku70 (XRCC6 Human)
    Tags
    GFP-FLAG
    Expression
    Mammalian
    Mutation
    Mut6E corresponding to K282E K287E T300E K331E K3…
    Promoter
    CMV Tet
    Available since
    Aug. 20, 2013
    Availability
    Academic Institutions and Nonprofits only
    46962-plasmid-map-sequence-id-66853

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  5. pXWJ109As(RS-te-AAV)

    Plasmid
    #123156
    Purpose
    For TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
    Depositor
    Baojun Wang
    Publication
    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d
    Insert
    J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J109, ParsR, PhrpLE
    Available since
    May 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    123156-plasmid-map-sequence-id-240600

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  8. pRSET a sfGCaMP6s

    Plasmid
    #100022
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.
    Depositor
    Wolf Frommer
    Publication
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfGCaMP6s
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP
    Promoter
    T7
    Available since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100022-plasmid-map-sequence-id-194291

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  12. JA524

    Plasmid
    #49938
    Purpose
    Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in EGFP for use as a control
    Depositor
    Keith Joung
    Publication
    Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038
    Insert
    Tet1CD GFP (TET1 Human)
    Use
    TALEN
    Promoter
    EF1a
    Available since
    Dec. 20, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. BAT8 (3K5K)

    Plasmid
    #25295
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    BAT8_08 (EHMT2 Human)
    Tags
    His
    Expression
    Bacterial
    Available since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pCoofy48

    Plasmid
    #55186
    Purpose
    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Publication
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    55186-plasmid-map-sequence-id-464535

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  16. pFUS-WT-VC

    Plasmid
    #175068
    Purpose
    expresses FUS wildtype tagged to mVenus and mCherry in mammalian cells
    Depositor
    Arnold Boersma
    Publication
    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018
    Insert
    FUS glycine rich protein (FUS Human)
    Tags
    HA and mVenus + mCherry
    Expression
    Mammalian
    Mutation
    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…
    Promoter
    CMV Promoter
    Available since
    Oct. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175068-plasmid-map-sequence-id-353791

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  17. pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant

    Plasmid
    #177130
    Purpose
    Generate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1
    Depositor
    Wenbo Li
    Publication
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTHDC1 Human)
    Use
    Lentiviral
    Tags
    mClover3
    Mutation
    W428A/W377A (m6A binding defects), and siRNA resi…
    Promoter
    Ef1a
    Available since
    July 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    177130-plasmid-map-sequence-id-366262

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Showing: 4741 - 4760 of 14356 results