We narrowed to 10,035 results for: pcDNA 3.1

1. pcDNA-NLS-PhoCl-mCherry

   Plasmid

   #87691

   Purpose

   Express NLS-PhoCl-mCherry in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   NLS-PhoCl-mCherry
   

   Use

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-LBR-EGFP-polyA

   Plasmid

   #59748

   Purpose

   Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   LBR-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-EGFP-ELYS-polyA

   Plasmid

   #59746

   Purpose

   Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   EGFP-ELYS
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-emerin-EGFP-polyA

   Plasmid

   #59747

   Purpose

   Express emerin-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   emerin-EGFP
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA-HA-Nedd4(C744E)/2797

   Plasmid

   #11206

   Depositor

   Allan Weissman

   Article

   Magnifico et al J Biol Chem. 2003 Oct 31. 278(44):43169-

   Insert

   Nedd4-1 (Nedd4 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   cysteine 744 mutated to glutamic acid

   Promoter

   Available since

   March 8, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA-LBR-mRFP1-polyA

   Plasmid

   #59749

   Purpose

   Express LBR-mRFP1 in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   LBR-mRFP1
   

   Use

   Tags

   mRFP1

   Expression

   Mammalian

   Mutation

   aa 1-249 of LBR

   Promoter

   CMV and T7

   Available since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-GFP-Dub3 mouse

   Plasmid

   #72683

   Purpose

   mammalian expression of GFP-Dub3

   Depositor

   Domenico Maiorano

   Article

   van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

   Insert

   Dub3 (Usp17le Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD005 pcDNA Golden Gate

   Plasmid

   #138749

   Purpose

   Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA mouse NHERF-1 (1-355)

   Plasmid

   #32705

   Depositor

   Edward Weinman

   Article

   Weinman et al Am J Physiol Renal Physiol. 2001 Aug;281

   Insert

   solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (Nherf1 Mouse)
   

   Use

   Tags

   none

   Expression

   Mammalian

   Mutation

   none

   Promoter

   cmv

   Available since

   Feb. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA-PhoCl-mCherry-myc

    Plasmid

    #87692

    Purpose

    Express PhoCl-mCherry with C-term myc tag in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    PhoCl-mCherry-myc
    

    Use

    Tags

    myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-NGFP-VMAn-FKBP

    Plasmid

    #70225

    Purpose

    Expresses N-terminal portion of GFP along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    eGFP
    

    Use

    Tags

    FK506- and rapamycin-binding protein (FKBP), Link…

    Expression

    Mammalian

    Mutation

    Changed Glu 125 to Ile, Deleted amino acids 129-2…

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-FRB-VMAc-CGFP

    Plasmid

    #70226

    Purpose

    Expresses C-terminal portion of GFP along with VMA intein for protein splicing

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    eGFP
    

    Use

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Ile 129 to Cys, Deleted amino acids 1-128

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3 SWS cone opsin

    Plasmid

    #109363

    Purpose

    Human SWS cone opsin with 1D4 tag

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    SWS cone opsin (OPN1SW Human)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-NBid-PhoCl1-CBid

    Plasmid

    #164050

    Purpose

    Optogenetic manipulation of cell apoptosis using PhoCl1

    Depositor

    Robert Campbell

    Article

    Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

    Insert

    NBid-PhoCl1-CBid
    

    Use

    Tags

    kozak sequence

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promotor

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-optoB2-LYY-YFP

    Plasmid

    #75271

    Purpose

    G-protein independent version of optoB2 with point mutations at L72F,Y136G,Y224A

    Depositor

    Michael Bruchas

    Article

    Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

    Insert

    G-protein independent version of optoB2
    

    Use

    Tags

    eYFP

    Expression

    Mammalian

    Mutation

    point mutations at L72F,Y136G,Y224A

    Promoter

    Available since

    Oct. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-FRB-VMAc-CSarcin

    Plasmid

    #70223

    Purpose

    Expresses VMA intein with C-terminal portion of alpha-sarcin

    Depositor

    Perry Howard

    Article

    Alford et al Mol Biosyst. 2014 Apr;10(4):831-7. doi:

    Insert

    alpha Sarcin (C-terminal portion)
    

    Use

    Tags

    FKBP-rapamycin binding (FRB) domain of the mammal…

    Expression

    Mammalian

    Mutation

    Changed Asp 28 to Cys, deleted amino acids 1-27

    Promoter

    CMV

    Available since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-FLAG-Fos Y345A

    Plasmid

    #8970

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y345A

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3-FLAG-Fos F343A

    Plasmid

    #8969

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    F343A

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

19. PCx35-pcDNA-S110A, S276A

    Plasmid

    #42926

    Depositor

    John O'Brien

    Article

    Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

    Insert

    Connexin 35
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Ser110 to Ala and Ser276 to Ala

    Promoter

    CMV

    Available since

    March 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA-HA-ER Y537S

    Plasmid

    #49499

    Purpose

    Expresses HA-tagged ER Y537S mutant in mammalian cells

    Depositor

    Sarat Chandarlapaty

    Article

    Toy et al Nat Genet. 2013 Dec;45(12):1439-45. doi:

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Tyrosine 537 to Serine

    Promoter

    CMV

    Available since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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