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Plasmid#228809PurposeCRISPR targeting of human EHMT1DepositorInsertgRNA targeting EHMT1 exon 3 (EHMT1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
EGFP_EHMT1B1-3
Plasmid#228811PurposeExpression of mouse EHMT1 with deletion of aa 1185-1298DepositorInsertEHMT1 (Ehmt1 Mouse)
UseTagsEGFPExpressionMammalianMutationdeletion of aa 1185-1298PromoterAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
EGFP_EHMT1B1-4
Plasmid#228812PurposeExpression of mouse EHMT1 with deletion of aa 809-1298DepositorInsertEHMT1 (Ehmt1 Mouse)
UseTagsEGFPExpressionMammalianMutationdeletion of aa 809-1298, See Depositor CommentsPromoterAvailable sinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-EF1+EF2
Plasmid#18080DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pYL192 (TRV1)
Plasmid#148968PurposeTobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)DepositorInsertTRV RNA1
UsePlant vector with trv rna1; this vector used with…TagsExpressionMutationP697S mutation in putative replicase. S239G and S…PromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPDK3876(TRV-RNA2-pPEBV-MCS)
Plasmid#149275PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of proteins or RNAsDepositorInsertModified TRV RNA2 with PEBV promoter
UseT-dna vectorTagsExpressionMutationT2318C (DNA) in PEBV coat protein sequencePromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7A-YFP
Plasmid#62634Purposemammalian expression of human duplicated Alpha7 (CHRFAM7A) with YFP fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsExpressionMammalianMutationYFP inserted in M3-M4 loopPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7-mCherry
Plasmid#62632Purposemammalian expression of human Alpha7 (CHRNA7) with mCherry fused into M3-M4 loopDepositorInserthAlpha7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationmCherry inserted into M3-M4 loopPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7
Plasmid#62276Purposemammalian expression of human Alpha7 (CHRNA7)DepositorInsertCHRNA7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 21, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Human a4 nicotinic subunit crystallization construct
Plasmid#85841PurposepEZT-BM vector with alpha4 gene after CMV promoter, published crystallization constructDepositorInsertHuman a4 nAChR subunit crystallization construct (CHRNA4 Human)
UseBaculovirus (bacmam) production and mammalian exp…TagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV CAMKII PSAM4 GlyR IRES EGFP
Plasmid#119744PurposeChemogenetic inhibitor expression plasmidDepositorUseAAVTagsExpressionMammalianMutationL131G, Q139L, Y217FPromoterCamKIIAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_MED13
Plasmid#111689PurposeMAC-tagged gene expressionDepositorInsertMED13 (MED13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV SYN flex PSAM4 GlyR IRES EGFP
Plasmid#119741PurposeCre-dependent chemogenetic inhibitor expressionDepositorUseAAVTagsExpressionMammalianMutationL131G, Q139L, Y217FPromotersynapsinAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 2xFLAG-2xSTREP RAD51 (T309A)
Plasmid#158953PurposeExpress human RAD51(T309A) mutant in mammalian cellsDepositorInsertRAD51 (RAD51 Human)
UseTags2X FLAG 2X STREPExpressionMammalianMutationchanged threonine 309 to alaninePromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)
Plasmid#158952PurposeExpress human RAD51(F129A) mutant in mammalian cellsDepositorInsertRAD51 (RAD51 Human)
UseTags2X FLAG 2X STREPExpressionMammalianMutationchanged phenylalanine 129 to alaninePromoterCMVAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV SYN PSAM4 GlyR IRES EGFP
Plasmid#119742PurposeChemogenetic inhibitor expression plasmidDepositorUseAAVTagsExpressionMammalianMutationL131G, Q139L, Y217FPromotersynapsinAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab8a-QL
Plasmid#86076PurposeA mammalian expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus EGFP.DepositorInserthRab8A (RAB8A Human)
UseTagsGFPExpressionMammalianMutationChanged glutamine 67 to leucinePromoterCMVAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Rab8a-TN
Plasmid#86077PurposeA mammalian expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus EGFP.DepositorInserthRab8A (RAB8A Human)
UseTagsGFPExpressionMammalianMutationChanged threonine 22 to asparginePromoterCMVAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
hMLKL-Venus-Plasma
Plasmid#106081Purposeexpression in mammalian cellsDepositorInserthMLKL-Venus-Plasma (MLKL Human)
UseRetroviral; Tet-on retrovirusTagsplasma membrane-targetting domain of H-Ras (aa 1-…ExpressionMammalianMutationPromoterdox-inducibleAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
His-SUMO-Rad23A_pET11a
Plasmid#169124Purposeexpression of proteinDepositorInserthRad23A (RAD23A Human)
UseTagsExpressionBacterialMutationwtPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRNA7-YFP
Plasmid#62630Purposemammalian expression of human Alpha7 (CHRNA7) with YFP fused into M3-M4 loopDepositorInserthAlpha7 (CHRNA7 Human)
UseTagsExpressionMammalianMutationYFP inserted in M3-M4 loopPromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEmpty
Plasmid#214051PurposeEmpty bacterial expression vector with CloDF13 (CDF) origin of replication; used as a negative control in the Haliangium ochraceum type III CRISPR-Cas systemDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
GST-Rab8a-QL
Plasmid#86079PurposeA bacterial expression plasmid encoding Rab8a GTPase-deficient mutant (Rab8a-Q67L) with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
UseTagsGSTExpressionBacterialMutationChanged glutamine 67 to leucinePromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-Rab8a-TN
Plasmid#86080PurposeA bacterial expression plasmid encoding GDP locked Rab8a (Rab8a-T22N) with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
UseTagsGSTExpressionBacterialMutationChanged threonine 22 to asparginePromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7ADelta2bp-mGFP
Plasmid#62636Purposemammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mEGFP fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsmEGFP inserted in M3-M4 loopExpressionMammalianMutationthere is a 2-bp deletion in exon 6 of CHRFAM7A ca…PromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7ADelta2bp-mCherry
Plasmid#62638Purposemammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mCherry fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsExpressionMammalianMutationthere is a 2-bp deletion in exon 6 of CHRFAM7A an…PromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET41a-MED30
Plasmid#15431DepositorInsertMED30 (Med30 Mouse)
UseTagsGST and HisExpressionBacterialMutationN/APromoterAvailable sinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-W3083F
Plasmid#18076DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationSecond conserved Tryptophan (W) of the WW domain …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-W3083F/P3086A
Plasmid#18077DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationSecond conserved Tryptophan (W) of the WW domain …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-d-ZZ-W3083F
Plasmid#18078DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationSecond conserved Tryptophan (W) of the WW domain …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2TK-WW-CR-d-ZZ-W3083F/P3086A
Plasmid#18079DepositorInsertDystrophin (DMD Human)
UseTagsGSTExpressionBacterialMutationSecond conserved Tryptophan (W) of the WW domain …PromoterAvailable sinceMay 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
SPDK3888(TRV-RNA2-pPEBV-MCS-AtIleu-tRNA)
Plasmid#149276PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAsDepositorInsertModified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
UseT-dna vectorTagsExpressionMutationT2318C (DNA) in PEBV coat protein sequencePromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF3187
Plasmid#144663PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRAD21 (RAD21 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
SPDK3889(TRV-RNA2-pPEBV-MCS-AtMet-tRNA)
Plasmid#149277PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAsDepositorInsertModified TRV RNA2 with PEBV promoter and AtMet-tRNA mobile RNA sequence
UseT-dna vectorTagsExpressionMutationT2318C (DNA) in PEBV coat protein sequencePromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)
Plasmid#149278PurposeModified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAsDepositorInsertModified TRV RNA2 with PEBV promoter and AtGly-tRNA mobile RNA sequence
UseT-dna vectorTagsExpressionMutationT2318C (DNA) in PEBV coat protein sequencePromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7ADelta2bp
Plasmid#62515Purposemammalian expression of human duplicated Alpha7 with 2 bp deletion (CHRFAM7A-∆2bp)DepositorInsertCHRFAM7ADelta2bp (CHRFAM7A Human)
UseTagsExpressionMammalianMutationpartially duplication of CHRNA7 with 2-bp deletio…PromoterCMVAvailable sinceMarch 20, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
FLAG-MED30
Plasmid#15426DepositorInsertMED30 (Med30 Mouse)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CHRFAM7ADelta2bp-YFP
Plasmid#62637Purposemammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with YFP fused into M3-M4 loopDepositorInsertCHRFAM7A (CHRFAM7A Human)
UseTagsExpressionMammalianMutationthere is a 2-bp deletion in exon 6 of CHRFAM7A an…PromoterCMVAvailable sinceMarch 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RAD50
Plasmid#116784PurposeLentiviral expression of RAD50DepositorInsertRAD50 (RAD50 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG PSAM4-5HT3 HC IRES EGFP
Plasmid#119740PurposeChemogenetic activator expression plasmidDepositorUseTagsExpressionMammalianMutationL131G, Q139L, Y217F, R420Q, R424D, R428APromoterCAGAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCH1/RAD51o
Plasmid#102562PurposeExpress Human RAD51 protein codon optimzed for E.coli and the E.coli GorE OperonDepositorInsertsHomo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
E. coli groE operon
UseTagsExpressionBacterialMutationCodon optimized for E.coli ExpressionPromoterT7Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEcNucC
Plasmid#214050PurposeBacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas systemDepositorInsertEcNucC
UseTagsExpressionBacterialMutationWTPromoterlacUV5 promoterAvailable sinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-3xFLAG Med24-exon minimal CMV pCDNA5
Plasmid#212060PurposeLR vector for integration of Med24-exon into N2a FRT rtTA3 expression cellsDepositorInsertMed24-exon (Med24 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
5'-3xFLAG Med24+exon minimal CMV pCDNA5
Plasmid#212061PurposeLR vector for integration of Med24+exon(57nt) into N2a FRT rtTA3 expression cellsDepositorInsertMed24+exon(57nt) (Med24 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFSF11-COMP5AP-AviTag-9xHis
Plasmid#157264PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertTNFSF11 (TNFSF11 Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORANGE GFP-CACNA1E KI
Plasmid#131481PurposeEndogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)DepositorUseTagsExpressionMammalianMutationPromoterU6 and CbhAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_MED13L_COL23A1
Plasmid#205849PurposeExpress mEGFP-tagged fusion protein, MED13L_COL23A1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-TNFSF11-Fc(DAPA)-AviTag-6xHis
Plasmid#156700PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertTNFSF11 (TNFSF11 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-N_MED16:1-860
Plasmid#211215PurposeMammalian expression of full-length human MED16 isoform 3. N-terminal HiBiT tag.DepositorInsertMED16:M1-P860 (MED16 Human)
UseTagsMVSGWRLFKKISGSSGGSSGExpressionMammalianMutationisoform 3PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_MED16:1-860
Plasmid#211289PurposeMammalian expression of full-length human MED16 isoform 3. C-terminal HiBiT tag.DepositorInsertMED16:M1-P860 (MED16 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only