-
Plasmid#201765PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-T1
Plasmid#201764PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-T3
Plasmid#201766PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-T4
Plasmid#201767PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c45
Plasmid#201771PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterTEF1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c46
Plasmid#201774PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterTEF1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c25
Plasmid#201769PurposeExpresses mNeonGreen-tagged NbALFA under CYC1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterCYC1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-c15
Plasmid#201768PurposeExpresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeastDepositorInsertNbALFA
UseTags40aa Linker, mNeonGreenExpressionYeastMutationPromoterADH1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Trex2
Plasmid#79246PurposeExpression vector for Trex2, which is a non-processive 3' exonuclease.DepositorInsertTrex2 (Trex2 Mouse)
UseTagsExpressionMammalianMutationPromoterpCAGGSAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-Bmpr2-1719
Plasmid#49379PurposeInsert is a fragment of the Bmpr2 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.DepositorInsertBmpr2 (BMPR2 Mouse, Human)
UseLuciferaseTagsExpressionMutationPromoterHuman phosphoglycerate kinase (PGK) promoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-Bmpr2-Δ17
Plasmid#49380PurposeInsert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-17 binding site. To be used for 3'UTR assay.DepositorInsertBmpr2 (BMPR2 Mouse, Human)
UseLuciferaseTagsExpressionMutationchanged nucleotides AC to TG at bp 7347-7348PromoterHuman phosphoglycerate kinase (PGK) promoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-Bmpr2-Δ19
Plasmid#49381PurposeInsert is a fragment of the Bmpr2 3'-UTR that contains a mutated miR-19 binding site. To be used for 3'UTR assay.DepositorInsertBmpr2 (BMPR2 Mouse, Human)
UseLuciferaseTagsExpressionMutationchanged nucleotides TG to AC at bp 7344-7345PromoterHuman phosphoglycerate kinase (PGK) promoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pCASB
Plasmid#190175PurposePlasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequenceDepositorInsertsgRNA cloning site
UseTagsExpressionBacterial and YeastMutationAdded 5'-agagacc-3' upstream and 5'…PromoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-blast-SCRAMBLE
Plasmid#26701Purpose3rd gen lentiviral shRNA expression vector containing scrambled shRNA sequence. Uses blasticidin for selectionDepositorInsertscramble
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
Flag-FOXC1
Plasmid#153114PurposeExpression of Flag tagged human FOXC1 in mammalian cellsDepositorInserthuman FOXC1 (FOXC1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-N-WASP
Plasmid#47406DepositorInsertN-WASP
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry-TIS11B CPM2
Plasmid#121143PurposeMammalian expression of mCherry tagged TIS11B CPM2 (charge pattern mutant 2)DepositorInsertZFP36L1 (TIS11B) charge pattern mutant 2 (VEGFA Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Myo10-∆PH
Plasmid#139781PurposeDetecting Myo10-∆PH by fluorescence microscopyDepositorInsertMyosin X (MYO10 Human)
UseTagsmCherryExpressionMammalianMutationDeletion of 3PH domainsPromoterCMVAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -