We narrowed to 3,869 results for: SPL

1. pJG103

   Plasmid

   #158533

   Purpose

   wrmScarlet11 x3 tandem repeats

   Depositor

   Cynthia Kenyon

   Article

   Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

   Insert

   wrmScarlet11 x3 tandem repeats
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pN1-CMV-Aff6_V18FdN-IntC-mCherryC-T2A-EGFP (C2)

   Plasmid

   #210725

   Purpose

   NIR optogenetic module for conditional protein splicing of split mCherry (C-part)

   Depositor

   Vladislav Verkhusha

   Article

   Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

   Insert

   C2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. PX852

   Plasmid

   #62884

   Purpose

   C-term SpCas9 piece of inducible split-4 (Cas9(C)-FKBP split-4).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa536-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS SV40, and NLS nucleoplasmin

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG-3C-sGFP11_pcDNA3.1(-)

   Plasmid

   #182932

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP11 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3C-cleavable split GFP 11, FLAG tag, and HA signa…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-3C-sGFP1-10_pcDNA3.1(-)

   Plasmid

   #182931

   Purpose

   For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

   Depositor

   William Weis

   Article

   Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

   Type

   Empty backbone

   Use

   Tags

   3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-HA-Nrxn1beta AS4(+)

   Plasmid

   #59410

   Purpose

   Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

   Depositor

   Peter Scheiffele

   Article

   Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

   Insert

   Nrxn1beta AS4(+) (Nrxn1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX854

   Plasmid

   #62886

   Purpose

   C-term SpCas9 piece of inducible split-5 (Cas9(C)-FKBP split-5).

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Insert

   SpCas9 (aa573-1368)
   

   Use

   CRISPR

   Tags

   FKBP12, NLS SV40, and NLS nucleoplasmin

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mTB3-BC2 nanobody

   Plasmid

   #210208

   Purpose

   Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

   Depositor

   Brian Kay

   Article

   Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

   Insert

   BC2 Nanobody
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTB3-Myc tag

   Plasmid

   #210205

   Purpose

   Type 3 phage-display vector that contains kanamycin resistance and displays the Myc-tag at the N-terminus of mature protein III.

   Depositor

   Brian Kay

   Article

   Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

   Insert

   Myc-tag
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Vector 1174D

    Plasmid

    #200012

    Purpose

    Fluorescent based sex separator in Aedes aegypti (SEPARATOR)

    Depositor

    Omar Akbari

    Article

    Weng et al bioRxiv. 2023 Jun 16:2023.06.16.545348.

    Insert

    Engineered dobulesex splicing module
    

    Use

    Synthetic Biology

    Tags

    Expression

    Insect

    Mutation

    Engineered dobulesex splicing module

    Promoter

    Hr5IE1

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRegev_V2

    Plasmid

    #204144

    Purpose

    Single reporter randomly selected from splicing library for biochemical validation.

    Depositor

    Oded Regev

    Article

    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    V2 exon in beta globin splicing minigene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated CAG

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRegev_V3

    Plasmid

    #204145

    Purpose

    Single reporter randomly selected from splicing library for biochemical validation

    Depositor

    Oded Regev

    Article

    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    V3 exon in beta globin splicing minigene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated CAG

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC19-P70a-Top-T~P70a-Bottom-T

    Plasmid

    #87314

    Purpose

    Expresses the modular Split-Broccoli system (Top + Bottom) under the control of E. coli sigma 70 promoters.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRegev_ExonSkip_LandingPad

    Plasmid

    #204186

    Purpose

    Backbone for cloning individual exon sequences into beta globin minigene splicing reporter.

    Depositor

    Oded Regev

    Article

    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

    Insert

    Beta globin splicing minigene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated CAG

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX-6P1-OPNb-delta S

    Plasmid

    #107984

    Purpose

    bacterial expression of OPN splice variant b

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    OPNb 1038bp-17aa signal (SPP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    WT splice variant

    Promoter

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-6P1-OPNc-delta S

    Plasmid

    #107985

    Purpose

    bacterial expression of OPN splice variant c

    Depositor

    Georg Weber

    Article

    He et al Oncogene. 2006 Apr 6;25(15):2192-202. do

    Insert

    OPNc 999bp-16aa signal (SPP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    WT splice variant

    Promoter

    Available since

    May 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19-P70a-Bottom-T

    Plasmid

    #87313

    Purpose

    Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system "Bottom" transcript
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19-Trigger-Bottom

    Plasmid

    #87317

    Purpose

    Expresses the "Bottom" transcript of the Split-Broccoli system downstream of an RNA Toehold switch Trigger sequence.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli "Bottom" transcript downstream of RNA Trigger (TS2_AT01)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC19-P70a-Top-T

    Plasmid

    #87312

    Purpose

    Expresses the "Top" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

    Depositor

    Donald Burke

    Article

    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

    Insert

    Split-Broccoli system "Top" transcript
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P70a

    Available since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTB3-FLAG tag

    Plasmid

    #210204

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the FLAG-tag at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    FLAG-tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge