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We narrowed to 63,780 results for: cloning

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Showing: 461 - 480 of 63780 results

  3. pSL1142 (pSPIN, pBBR1 backbone)

    Plasmid
    #160730
    Purpose
    Single-plasmid V. cholerae CAST system, encodes all proteins, crRNA, and donor DNA. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Publication
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    160730-plasmid-map-sequence-id-357931

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  6. pMOD_B2515b

    Plasmid
    #91073
    Purpose
    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Promoter
    AtU6
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91073-plasmid-map-sequence-id-181900

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  8. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60231
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60231-plasmid-map-sequence-id-417804

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  10. pMTMVi-M

    Plasmid
    #118757
    Purpose
    Intermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.
    Depositor
    Jose-Antonio Daros
    Publication
    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10
    Insert
    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    Use
    Intermediate to build tobacco mosaic virus recomb…
    Available since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    118757-plasmid-map-sequence-id-231795

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  12. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Publication
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90085-plasmid-map-sequence-id-182691

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  14. pSL1521 (pSPIN, pSC101* backbone)

    Plasmid
    #160729
    Purpose
    Single-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.
    Depositor
    Samuel H. Sternberg
    Publication
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    Jan. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    160729-plasmid-map-sequence-id-322926

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  16. pEM040 [Multiome Perturb-seq sgRNA expression vector]

    Plasmid
    #224898
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments
    Depositor
    Thomas Norman
    Publication
    Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    224898-plasmid-map-sequence-id-443622

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  17. pMOD_C2516b

    Plasmid
    #91085
    Purpose
    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Promoter
    At7SL
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91085-plasmid-map-sequence-id-182050

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  19. OgeuIscB ωRNA-v13

    Plasmid
    #219634
    Purpose
    human U6-driven expression of OgeuIscB omegaRNA-v13 (ωRNA-v13) scaffold with BsmBI for guide cloning
    Depositor
    Weixin Tang
    Publication
    Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    219634-plasmid-map-sequence-id-443112

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Showing: 461 - 480 of 63780 results