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Plasmid#166426PurposeRab5C fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.DepositorInsertRab5C
UseTagsmRuby3ExpressionMammalianMutationPromoterL30 weak promoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK369
Plasmid#73594PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitopeDepositorInsertdelta-12 fatty acid acetylenase
UseTagsFLAG tagExpressionYeastMutationPromoterGAL10Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
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mCherry-Talin-ABM-10
Plasmid#55138PurposeLocalization: Talin Actin Binding Domain, Excitation: 587, Emission: 610DepositorInsertTalin ABM
UseTagsmCherryExpressionMammalianMutationActin Binding Module; I/LWEQ = 197 amino acids fr…PromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
mApple-Tubulin-6
Plasmid#54956PurposeLocalization: Microtubules, Excitation: 568, Emission: 592DepositorInsertTubulin (TUBA1B Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAMTA2-donor
Plasmid#132501PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertCAMTA2 (CAMTA2 Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
mApple-Tensin1-N-18
Plasmid#54953PurposeLocalization: Focal Adhesions, Excitation: 568, Emission: 592DepositorInsertTensin1 (TNS1 Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
mApple-Talin-ABM-10
Plasmid#54950PurposeLocalization: Talin Actin Binding Domain, Excitation: 568, Emission: 592DepositorInsertTalin ABM
UseTagsmAppleExpressionMammalianMutationActin Binding Module; I/LWEQ = 197 amino acids fr…PromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE-miRFP703_PGK-Puro
Plasmid#216667PurposeExpresses tTa-inducible miRFP703, with puromycin resistanceDepositorInsertsmiRFP703
PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-phSyn-flex-SypHaloTag
Plasmid#172280PurposeCre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.DepositorInsertsynaptophysin-HaloTag
UseAAV and Cre/LoxTagsHaloTagExpressionMammalianMutationPromoterrat synapsinAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4
Plasmid#219711PurposeLevel 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.DepositorInsertGG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAMTA2.1.0-gDNA
Plasmid#132456PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertCAMTA2 (CAMTA2 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMBD1.1.0-gDNA
Plasmid#132444PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertMBD1 (MBD1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK523
Plasmid#72442PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes, with N-terminal His6 tag (H6AaOrfX)DepositorInserthypothetical protein
UseTagsHis6ExpressionBacterialMutationsynthetic gene with ~40 silent substitutionsPromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK138
Plasmid#73314PurposePas2p peroxisomal membrane targeting sequenceDepositorInsertPas2p (peroxisomal membrane targeting region)
UseTagsHis6 and c-mycExpressionYeastMutationencodes residues 1-42PromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK126
Plasmid#73337PurposeEncodes Mycobacterium tuberculosis FdxB (C-terminal flavoprotein reductase domain)DepositorInsertelectron transfer flavoprotein
UseTagsHis6ExpressionBacterialMutationencodes residues 328-685PromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK108
Plasmid#73312PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2/synthetic)DepositorInsertdelta-12 fatty acid acetylenase
UseTagsExpressionBacterialMutationsynthetic genePromoterT7Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK322
Plasmid#73494PurposeProduces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes and N5-carboxyaminoimidazole ribonucleotide mutaseDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
UseTagsExpressionBacterialMutationPromotern/aAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK125
Plasmid#73336PurposeEncodes Mycobacterium tuberculosis FdxB (N-terminal fatty acid desaturase domain)DepositorInsertelectron transfer flavoprotein
UseTagsHis6ExpressionBacterialMutationencodes residues 1-327PromoterT7Available sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK553
Plasmid#72452PurposeProduces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1)DepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
UseTagsHis6ExpressionBacterialMutationsynthetic gene with ~40 silent substitutionsPromoterT7Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only