We narrowed to 7,283 results for: ef1a

1. Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA

   Plasmid

   #97312

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. Lenti backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb HMEJ donor
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-CDreV

   Plasmid

   #140133

   Purpose

   can be used to generate AAV virus that will express fusion protein of split Dre (C-terminal) and fungal photoreceptor Vivid (VVD) monomer

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Insert

   CDreV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-EF1a-KLF4

   Plasmid

   #35389

   Depositor

   Feng Zhang

   Article

   Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

   Insert

   TALE-Klf4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pEF1a-2xSV40_NLS-CeNLuc

   Plasmid

   #135952

   Purpose

   Expresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   CeNLuc LumiFluor
   

   Use

   Tags

   2xSV40-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSMPUW_6.11_M3_Ef1a-gateway-HA-SV40

   Plasmid

   #128447

   Purpose

   lentiviral destination vector with Ef1a promoter, C-terminal HA tag, and SV40 promoter

   Depositor

   Lindy Barrett

   Insert

   Ef1a promoter, C-terminal HA tag, and SV40 promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE2-EF1alpha-HA-TFAP4

   Plasmid

   #110158

   Purpose

   Lentiviral vector for expression of human TFAP4 in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

   Insert

   TFAP4 (transcription factor AP-4) (TFAP4 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. MS2392.EF1a.SLC6A1.WT.P2A.BLAST.JL.p159

   Plasmid

   #170170

   Purpose

   Lentiviral Expression of EF1a.SLC6A1.WT

   Depositor

   Christopher Moxham

   Insert

   SLC6A1 (SLC6A1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   n/a

   Promoter

   Available since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-EF1a-DIO-HB

   Plasmid

   #37452

   Depositor

   Edward Callaway

   Insert

   Histone, 2A element, rabies B19 glycoprotein
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

9. Ef1a-QCP-3xBFP

   Plasmid

   #158206

   Purpose

   Qb coat protein fused to three repeats of the blue fluorescent protein under a Efla promoter.

   Depositor

   Roee Amit

   Article

   Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

   Insert

   QCP under the Elfa promoter and three BFP tags
   

   Use

   Synthetic Biology

   Tags

   3xBFP

   Expression

   Mammalian

   Mutation

   Promoter

   Elfa

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-EF1a-OCT4

    Plasmid

    #35391

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TALE-Oct4
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pRRL.SIN.EF1A.JAG1-F1.218.CAR-2G

    Plasmid

    #194459

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-JAG1 CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-pEF1a-rtTAc

    Plasmid

    #172121

    Purpose

    Constitutive expression of InteinC-rtTAC

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    InteinC-rtTAc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-EF1a-V5-Vrk2

    Plasmid

    #120240

    Purpose

    Expresses V5-tagged Vrk2 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Vrk2 (Vrk2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. cEF1a-LSL-GFP

    Plasmid

    #135672

    Purpose

    Introduce Cre-inducible (EF1a promoter followed by lox-stop-lox) GFP cDNA into (ES) cells by recombination-mediated cassette exchange

    Depositor

    Florian Karreth

    Insert

    GFP
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEF1alpha-rTetR-bGHpA

    Plasmid

    #172285

    Purpose

    Constitutive expression of reverse tetracycline-controlled transactivator

    Depositor

    Patrik Verstreken

    Insert

    rTetR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

    Plasmid

    #97310

    Purpose

    NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb NHEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, EF1a

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MS2392.EF1a.SLC6A1.WT.EGFP.P2A.BLAST.JL.p164

    Plasmid

    #170169

    Purpose

    Lentiviral Expression of EF1a.SLC6A1.WT.EGFP

    Depositor

    Christopher Moxham

    Insert

    SLC6A1 (SLC6A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV-EF1a-V5-Emerin

    Plasmid

    #120246

    Purpose

    Expresses V5-tagged Emerin in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Emerin (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EF1a-EGFP-KANL

    Plasmid

    #170164

    Purpose

    Mammalian Expression of Ef1a-EGFP-KANL

    Depositor

    Christopher Moxham

    Insert

    EGFP-KANL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    KANL

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LV_EF1a_CDK6-P2A-Hygro_Barcode

    Plasmid

    #170224

    Purpose

    Barcoded lentiviral vector to express CDK6 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CDK6 (CDK6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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