We narrowed to 921 results for: pgem

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p177bp-LacOx50-Hyg

Plasmid

#210213

Purpose

Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Publication

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pFH6_new

Plasmid

#105866

Purpose

more efficient version of pFH6; Subcloning of any sgRNA via BbsI site

Depositor

Andreas Weber

Publication

Hahn et al Bio Protoc. 2017 Jul 5;7(13):e2384. doi:

Insert

U6-26p::sgRNA scaffold

Use

CRISPR and Synthetic Biology; Subcloning

Expression

Plant

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSLI-N-GFP-2xFKBP-loxP(K13)

Plasmid

#85792

Purpose

P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI

Depositor

Tobias Spielmann

Publication

Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

Kelch13

Use

Cre/Lox; P. falciparum genomic integration

Tags

2xFKBP, GFP

Promoter

none

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRAM18dRGA[MCS]

Plasmid

#84683

Purpose

Can be used for the transformation of rickettsia

Depositor

Ulrike Munderloh

Publication

Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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K18iresCreER-T2

Plasmid

#44580

Depositor

Robert Oshima

Publication

Wen et al Genesis. 2003 Feb;35(2):100-6.

Insert

keratin 18 gene (KRT18 Human)

Use

Mouse Targeting

Tags

IRES_CreER-T2

Available since

June 18, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pGolf-ISH

Plasmid

#15853

Depositor

Peter Mombaerts

Publication

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Guanine Nucleotide Binding Protein, Alpha Stimulating, Olfactory type (Gnal Mouse)

Use

Cloning vector

Mutation

F199S

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pGMβ1

Plasmid

#216202

Purpose

Chromosomal gene manipulation (gene insertion, conversion, deletion) of dairy used Lactobacillus bulgaricus, a difficult gene to manipulate, is now possible by conjugation using this pGMB1 plasmid.

Depositor

Yasuko Sasaki

Publication

Iwamoto et al Biosci Microbiota Food Health. 2022;41(1

Type

Empty backbone

Use

Shuttle vector, conjugal plasmid, theta type rep…

Expression

Bacterial

Promoter

lac promoter in pGEM-Teasy,　Promoters in pAMbeta1…

Available since

Jan. 21, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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mouse TF cDNA

Plasmid

#15454

Depositor

Nigel Mackman

Publication

Mackman et al FASEB J. 1995 Jul . 9(10):883-9.

Insert

Tissue factor (F3 Mouse)

Use

Luciferase

Expression

Bacterial

Available since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
CRISPR-Cas9 Gateway System for Physcomitrella patens

Plasmid kit

#1000000151

Purpose

System for genome editing of the moss Physcomitrella patens.

Depositor

Magdalena Bezanilla

Application

Genome Editing

Vector type

Plant Expression

Editing type

CRISPR

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only
pAC3-ISH

Plasmid

#15854

Depositor

Peter Mombaerts

Publication

Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

Insert

Adenylate Cyclase 3 (Adcy3 Mouse)

Expression

Bacterial

Mutation

contains residues 787-1021

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pR-BAM

Plasmid

#22649

Depositor

Lynn Hudson

Publication

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1L (Myt1l Rat)

Use

Cloning and rna synthesis

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pλPR cI-Elysis

Plasmid

#176891

Purpose

Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

Depositor

James Inglese

Publication

Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

Insert

Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system

Expression

Bacterial

Promoter

T7

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-Hyg

Plasmid

#210261

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a hygromycin drug selection marker downstream of the promoter.

Depositor

Calvin Tiengwe

Publication

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMH-Cas9-gate

Plasmid

#113742

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

Depositor

Magdalena Bezanilla

Publication

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR and Gateway Cloning

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pISH-cre

Plasmid

#15933

Depositor

Peter Mombaerts

Publication

Li et al Nature. 2004 Mar 25. 428(6981):393-9.

Insert

cre recombinase (cre )

Mutation

642C to T, 966A to G

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
C035

Plasmid

#174341

Purpose

Template for making Rluc mRNA reporter with pA120.

Depositor

David Bartel

Publication

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

Renilla luciferase

Use

In vitro transcription template

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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pRAM18dSFA[MCS]

Plasmid

#84685

Purpose

Can be used for the transformation of rickettsia

Depositor

Ulrike Munderloh

Publication

Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

Type

Empty backbone

Expression

Bacterial

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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prDNA spacer-LacOx120-20xMS2-Hyg

Plasmid

#210220

Purpose

Integrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Publication

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

120xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Gfp-zf-vasa

Plasmid

#167323

Purpose

Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

Depositor

Goro Yoshizaki

Publication

Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

Insert

zf vasa 3'UTRs (ddx4 Zebrafish)

Expression

Bacterial

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRU48

Plasmid

#167670

Purpose

ENTRY vector for gRNA8 cloning under pAtU3 promoter

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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