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We narrowed to 8,583 results for: construct

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Showing: 4781 - 4800 of 8583 results

  1. pSBbi-Bla-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196074
    Purpose
    (Empty Backbone) Constitutive CRISPRi vector conferring blasticidin S resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196074-plasmid-map-sequence-id-389001

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  2. SB39

    Bacterial Strain
    #235121
    Purpose
    E. coli strain suitable for cloning, expression, library construction (good electroporation yield) and quantitative bacterial two-hybrid (qB2H).
    Depositor
    Oscar Ramos
    Bacterial Resistance
    None
    Available Since
    Oct. 27, 2025
    Availability
    Academic Institutions and Nonprofits only

  4. KARS_pNIC-Bio3

    Plasmid
    #153051
    Purpose
    Expression construct for producing Avi tagged Lysyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    KARS (KARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153051-plasmid-map-sequence-id-304466

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  6. pTrcHisA-PPARalpha(aa101-468)

    Plasmid
    #13574
    Depositor
    Martin Scanlon
    Insert
    human peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468) (PPARA Human)
    Tags
    x6His N-terminal, enterokinase cleavage site
    Expression
    Bacterial
    Mutation
    deleted residues 1-100.
    Available Since
    March 15, 2007
    Availability
    Academic Institutions and Nonprofits only
    13574-plasmid-map-sequence-id-142611

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  7. MSCV-mMDM2-IRES-GFP

    Plasmid
    #66954
    Purpose
    retroviral construct for expression of mouse Mdm2 cloned into the EcoRI site
    Depositor
    Martine Roussel
    Insert
    Mdm2 (Mdm2 Mouse)
    Use
    Retroviral
    Tags
    IRES-GFP
    Expression
    Mammalian
    Promoter
    MSCV
    Available Since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66954-plasmid-map-sequence-id-173086

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  9. pUPD2 PhyB (GB2721)

    Plasmid
    #160603
    Purpose
    chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch.
    Depositor
    Diego Orzaez
    Insert
    PhyB
    Use
    Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160603-plasmid-map-sequence-id-322052

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  10. HARS_pNIC-Bio3

    Plasmid
    #153050
    Purpose
    Expression construct for producing Avi tagged histidyl tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    HARS (HARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153050-plasmid-map-sequence-id-304465

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  13. pPB-PGK-mCherry.OVA(323-339)

    Plasmid
    #190005
    Purpose
    Piggybac construct for constitutive expression of mCherry and Chicken Ovalbumin OVA323-339 peptide fusion
    Depositor
    Siyuan Zhang
    Insert
    mCherry.OVA(323-339)
    Use
    Synthetic Biology; Piggybac
    Tags
    OVA(323-339)
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    190005-plasmid-map-sequence-id-382848

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  14. pMbd3-GGSG-AID-3xF_PP

    Plasmid
    #235507
    Purpose
    Targeting construct to make C-term fusion with GGSG-AID-3xFLAG, roxed Pgk-Puro
    Depositor
    Brian Hendrich
    Insert
    Mbd3 (Mbd3 Mouse)
    Use
    Mouse Targeting
    Tags
    AID, 3xFLAG
    Mutation
    None
    Promoter
    PGK
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235507-plasmid-map-sequence-id-474013

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  15. pChd8_GGSG_Fkbp_2xHA_BB

    Plasmid
    #235524
    Purpose
    Targeting construct: Chd8-FKBP-HA2 with Roxed Blasto cassette
    Depositor
    Brian Hendrich
    Insert
    Chd8 (Chd8 Mouse)
    Use
    Mouse Targeting
    Tags
    FKBP, 2xHA
    Mutation
    None
    Promoter
    beta actin
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    235524-plasmid-map-sequence-id-473993

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  16. L1PB26- pAHP6--PhiC31-NLS-tUBQ1 + pGATA23--Bxb1-SAUR-tUBQ10

    Plasmid
    #219701
    Purpose
    Level 1 PhiC31 and Bxb1 integrase construct for tracking of lateral root development: pAHP6 promoter for PhiC31 and pGATA23 promoter for Bxb1, NLS tag for PhiC31 and RNA degradation tag for Bxb1
    Depositor
    Jennifer Nemhauser
    Insert
    pAHP6::PhiC31-NLS:tUBQ1 + pGATA23::Bxb1-SAUR:tUBQ10
    Expression
    Plant
    Available Since
    Oct. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    219701-plasmid-map-sequence-id-435731

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  17. pT2/shαSMA/GFP4_Seq1/clone1

    Plasmid
    #206138
    Purpose
    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    alpha-SMA (Acta2 Mouse)
    Expression
    Mammalian
    Available Since
    Nov. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    206138-plasmid-map-sequence-id-410128

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  18. pCASCADE-gltA1-gltA2-udhA-zwf

    Plasmid
    #87152
    Purpose
    gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-udhA-zwf guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87152-plasmid-map-sequence-id-174941

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  19. pCASCADE-fabI-gltA1-gltA2-gapAP1

    Plasmid
    #87147
    Purpose
    fabI-gltA1-gltA2-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    fabI-gltA1-gltA2-gapAP1 guide array
    Expression
    Bacterial
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87147-plasmid-map-sequence-id-174935

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Showing: 4781 - 4800 of 8583 results