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Plasmid#172768PurposeBacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-2
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shTREX1
Plasmid#127700PurposeDoxycyclin inducible shRNA knockdown of both human and mouse TREX1 geneDepositorInsertshTREX1 (Trex1 Human, Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-18
Plasmid#172763PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-18
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)
Plasmid#86416PurposeONM/ER split GFP11 reporter with mCherryDepositorInsertNOP1pr-GFP11-mCherry-Scs2TM
UseTagsGFP11 and mCherryExpressionYeastMutationPromoterNOP1Available sinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDR-GFPuniv
Plasmid#46085PurposeIn vivo recombination assay fluorescent reporterDepositorInsertDR-GFPuniv reporter
UseRecombination reporter plasmidTagsExpressionMammalianMutationE5G and K163R mutations in EGFP relative to wild-…PromoterAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2-PhoCl-Cre-PhoCl-ERT2
Plasmid#87694PurposeExpress ERT2-PhoCl-Cre-PhoCl-ERT2 in mammalian cellsDepositorInsertERT2-PhoCl-Cre-PhoCl-ERT2
UseTagsExpressionMammalianMutationPromoterChicken beta actinAvailable sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pROSA26-promoter
Plasmid#21710DepositorInsertROSA26 promoter (Gt(ROSA)26Sor Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-42
Plasmid#172758PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-42
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY011 (pcDNA3.1-hLb2Cpf1)
Plasmid#69983PurposeExpresses humanized Lb2Cpf1DepositorInserthLb2Cpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluSnFR
Plasmid#106123PurposeIndicator for glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR
UseTagsMyc and PDGFRExpressionMammalianMutationPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-nuc-Nb37
Plasmid#205515PurposeNuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.DepositorInsertnuc-Nb37
UseTagsNuclear localization signal (NLS) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTU424
Plasmid#178983PurposepACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)DepositorInsertTPR-CHAT
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
CANE-LV envelope
Plasmid#86666PurposepCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirusDepositorInsertCANE-LV envelope
UseLentiviral, Mouse Targeting, and Synthetic Biolog…TagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-6
Plasmid#172741PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-6
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-KSENB-Nup133
Plasmid#87340PurposeTo express human Nup133 in mammalian cellsDepositorInsertNUP133 (NUP133 Human)
UseTagsEGFPExpressionMammalianMutationdeletion of Nup133 AA1-7PromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN80
Plasmid#61349PurposeLINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.DepositorTypeEmpty backboneUseTagsAsLOV2 wt and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-ExRai-AMPKAR
Plasmid#192450PurposeExRai-AMPKAR targeted to the nucleus.DepositorInsertExRai-AMPKAR-NLS
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pN256
Plasmid#188472PurposescDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFPDepositorInserteGFP-WPRE-DAISY-4T
UseTagsExpressionMutationPromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1.ARRDC1
Plasmid#38320DepositorInsertARRDC1 (ARRDC1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
SuExp-mPLD3
Plasmid#201245Purposeexpress full length mouse PLD3 proteinDepositorInsertPLD3 (Pld3 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only