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pCB268

Plasmid

#53363

Purpose

pcDNA3.0-based plasmid encoding fDHFR-UbK48R-Aβ42 13myc under the control of T7 or CMV promoter for 35S-pulse-chase URT-based assays in rabbit reticulocyte extract.

Depositor

Alexander Varshavsky

Publication

Brower et al Mol Cell. 2013 Apr 25;50(2):161-71. doi:

Inserts

DHFR (Dhfr Mouse)
Ub
Aβ42 (APP Human)

Tags

13xMyc, Flag, and HA

Expression

Mammalian

Promoter

CMV

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pECE-HA-AMPKalpha2 M93G

Plasmid

#31655

Depositor

Anne Brunet

Publication

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

protein kinase, AMP-activated, alpha 2 catalytic subunit M93G (PRKAA2 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

changed Methionine 93 to Glycine

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
-962 human cyclin D1 promoter CREB site mutant pGL3Basic

Plasmid

#32732

Depositor

Frank McCormick

Publication

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CCND1 Promoter (CCND1 Human)

Use

Luciferase

Mutation

-51 CREB site TAACGTCA deletion

Promoter

-962 CCND1 Promoter

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLG032 (ppl)

Plasmid

#157910

Purpose

Expresses the ppl defense system

Depositor

Feng Zhang

Publication

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

ppl (PHP-ATPase)

Expression

Bacterial

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CROPseq-tRFP

Plasmid

#230938

Purpose

Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

Depositor

Hyejung Won

Publication

Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Available since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Publication

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Promoter

CMV

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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neurod1:Gal4; cmlc2:EGFP

Plasmid

#173478

Purpose

Gal4 is driven by -5kb neurod1 promoter. The vector contains a cmlc2:EGFP marker

Depositor

John Rawls

Publication

Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

Insert

Gal4

Use

Zebrafish expression

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGCS-N5(GST)

Plasmid

#85722

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Publication

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

GST

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pGCS-C5(GST)

Plasmid

#85728

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Publication

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

GST

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAG61

Plasmid

#35129

Depositor

John McCusker

Publication

Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Insert

URA3

Use

Yeast replicating

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmVirD2

Plasmid

#90314

Purpose

mVirD2 expression plasmid

Depositor

Bjorn Hogberg

Publication

Bernardinelli et al Nucleic Acids Res. 2017 Oct 13;45(18):e1

Insert

A. tumefaciens VirD2 from aa 1 to 204

Tags

His6x tag

Expression

Bacterial

Promoter

T7/T5 IPTG inducible

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRF-BM3R1

Plasmid

#49358

Depositor

Christopher Voigt

Publication

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

BM3R1 repressor

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO-shCIAP2-B

Plasmid

#44132

Depositor

William Hahn

Publication

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

CIAP2

Use

Lentiviral and RNAi

Available since

March 29, 2013

Availability

Academic Institutions and Nonprofits only
pMX156

Plasmid

#29482

Depositor

Linda Wordeman

Publication

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

Kif2A-beta (Kif2a Mouse)

Tags

EGFP

Expression

Mammalian

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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V7 pDNR Dual - Donor

Plasmid

#11689

Depositor

Tony Pawson

Publication

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pALPS tat-P2A-rev

Plasmid

#101331

Purpose

Lentivector expressing codon optimized NL4-3 tat and rev linked by P2A peptide coding sequence (GSGATNFSLLKQAGDVEENPGP)

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 tat and rev

Use

Lentiviral

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBW213ara-hrpS

Plasmid

#61435

Purpose

pBAD18-cm encoding rbs33-hrpS-ter

Depositor

Martin Buck, Baojun Wang

Publication

Wang et al Nat Commun. 2011 Oct 18;2:508. doi: 10.1

Insert

rbs33-hrpS-ter

Expression

Bacterial

Promoter

pBAD

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pPL5617_pUG_PuroR

Plasmid

#60928

Purpose

PCR template vector for generating puromycin resistance cassette.

Depositor

Robert Piper

Publication

MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

Insert

puromycin N-Acetyltransferase

Use

Loxp flanked deletion cassette template

Promoter

TEF1

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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integrin alpha9 pcDNA1 neo

Plasmid

#13585

Depositor

Dean Sheppard

Publication

Yokosaki et al J Biol Chem. 1994 Oct 28. 269(43):26691-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

integrin alpha 9 (ITGA9 Human)

Expression

Mammalian

Available since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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Rogi2-CMV-mRuby-bGH

Plasmid

#179861

Purpose

Targets Rogi2 genomic region with HDR and expresses mRuby

Depositor

Sai Reddy

Publication

Aznauryan et al Cell Rep Methods. 2022 Jan 14;2(1):10015

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mRuby

Available since

Feb. 8, 2022

Availability

Academic Institutions and Nonprofits only

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