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pPAC

Plasmid

#165837

Purpose

puromycin resistance marker

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

pac

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pL3S2P21_STD

Plasmid

#165805

Purpose

Synthetic bacterial terminator

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

L3S2P21

Expression

Bacterial

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pspy_STD

Plasmid

#165806

Purpose

bacterial terminator

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

spy

Expression

Bacterial

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD111

Plasmid

#172957

Purpose

pRMCD77 encoding Wzz mutant G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G311A mutant gene

Expression

Bacterial

Mutation

D168G and N33D- please see depositor comment

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD113

Plasmid

#172959

Purpose

pRMCD77 encoding Wzz mutant G305A/G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G305A/G311A mutant gene

Expression

Bacterial

Mutation

G305A/G311A, A208V - please see depositor comments

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBL-2

Plasmid

#162769

Purpose

Minimal backbone with AmpR

Depositor

Ivan Vorobiev

Article

Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

Type

Empty backbone

Use

Synthetic Biology; Molecular cloning

Promoter

no

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pG-02

Plasmid

#166933

Purpose

Target AD1.A5 part (G)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Star sense AD1.S5 +RBS - Block G- Flanked by BsaI restriction sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pC-03

Plasmid

#166927

Purpose

STAR-AD1.A6 part (C)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Star Antisense AD1.A6 - Block C - Flanked by BsaI sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pC-02

Plasmid

#166926

Purpose

STAR-AD1.A5 part (C)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Star Antisense AD1.A5- Block C- Flanked by BsaI restriction sites

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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MvChR1_EYFP_pcDNA3.1

Plasmid

#159119

Purpose

This plasmid encodes MvChR1 rhodopsin domain

Depositor

John Spudich

Article

Govorunova et al mBio. 2011 Jun 21;2(3):e00115-11. doi: 1

Insert

MvChR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pHC_DYOLacI-R

Plasmid

#102922

Purpose

pUC-19 derivative reporter plasmid for the screening of Transcription Factors (TFs) carrying the DNA-binding domain of LacI

Depositor

George Church

Article

Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

Insert

sfGFP

Available Since

Aug. 13, 2020

Availability

Academic Institutions and Nonprofits only

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relAE335Q_NS1

Plasmid

#100917

Purpose

expresses E.coli relAE335Q from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

RelA fragment from E.coli with point mutation E335Q

Use

Cyanobacteria cloning vector

Tags

FLAG

Mutation

E 335 converted to Q

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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LI A-B phrpB

Plasmid

#104514

Purpose

LI promoter module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

hrpB promoter

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI A-B pEPS

Plasmid

#104515

Purpose

LI promoter module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

eps promoter

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI A-B pGala7

Plasmid

#104516

Purpose

LI promoter module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Gala7 promoter

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI C-D Dest

Plasmid

#104518

Purpose

LI Gateway cassette

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Gateway cassette

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI D-E V5

Plasmid

#104519

Purpose

LI C-terminal module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

3*V5 gene

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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LI E-F Tfd

Plasmid

#104520

Purpose

LI terminator module

Depositor

Thomas Lahaye

Article

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Tfd terminator

Use

Cloning vector

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) short BP-3'SS

Plasmid

#111333

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Mutation

shorter branch to 3' splice site distance

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pOBCol2.3MCSpA

Plasmid

#109485

Purpose

Drive inserted gene in osteoblasts.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Type

Empty backbone

Tags

None

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Nqo1

Plasmid

#105992

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Nqo1 (Nqo1 Mouse)

Use

To generate riboprobe

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSM2

Plasmid

#85753

Purpose

pDONR split marker vector (GenBank accession number: KX904529) for fungal gene deletion. Used with pSM1.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Expression

Bacterial

Promoter

N/A

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSM1

Plasmid

#85752

Purpose

pDONR split marker vector (GenBank accession number: KX904528) for fungal gene deletion. Used with pSM2.

Depositor

Damien Fleetwood

Article

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Expression

Bacterial

Promoter

N/A

Available Since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Ppj220(JBEI-13483)

Plasmid

#89786

Purpose

for expression of C-StrepII-3607

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

3607

Tags

StrepII

Expression

Bacterial

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006820

Plasmid

#87921

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste2803

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006818

Plasmid

#87924

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste3608

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006826

Plasmid

#87923

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste3608

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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JPUB_006834

Plasmid

#87920

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste3608

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
JPUB_006828

Plasmid

#87922

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste2803

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
JPUB_006812

Plasmid

#87925

Purpose

E. coli expression of K. stuttgartiensis protein

Depositor

Harry Beller

Article

Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

Insert

Kuste2803

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 4, 2017

Availability

Academic Institutions and Nonprofits only

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psglessClonePX

Plasmid

#87193

Purpose

used for downstream cloning, contains CMV promoter with NheI and XhoI cloning sites flanking a partial insert that gets excised when adding an insert of choice

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pNR292

Plasmid

#79548

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP. Implements RSM with 3-input passcode behavior.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

mTagBFP

Expression

Bacterial

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Zfhx4

Plasmid

#84324

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Zfhx4. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Zfhx4 (Zfhx4 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Phtf1

Plasmid

#84318

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Phtf1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Phtf1 (Phtf1 Mouse)

Available Since

Nov. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pNR166

Plasmid

#79547

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Stmn2

Plasmid

#74397

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Stmn2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

stathmin-like 2 (Stmn2 Mouse)

Available Since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Tgif1

Plasmid

#74359

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Tgif1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

TGFB-induced factor homeobox 1 (Tgif1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Zhx1

Plasmid

#74364

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Zhx1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

zinc fingers and homeoboxes 1 (Zhx1 Mouse)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Six1

Plasmid

#74358

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Six1. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

sine oculis-related homeobox 1 (Six1 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Pbx3

Plasmid

#74352

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pbx3. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

pre B cell leukemia homeobox 3 (Pbx3 Mouse)

Available Since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

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