We narrowed to 9,370 results for: sacs

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pIM23

Plasmid

#74643

Purpose

Centromeric plasmid to construct episomal C-terminal fusions of genes with yeast-adapted TagRFP-T and HIS3 selection marker

Depositor

Jiří Hašek

Article

Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

Insert

yTagRFP-T

Expression

Yeast

Promoter

MET25

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCfB8786

Plasmid

#126920

Purpose

Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-Osh1

Plasmid

#58835

Purpose

Expresses GFP-Tagged Osh1 in yeast

Depositor

Tim Levine

Article

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Osh1 (SWH1, OSH1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

PHO5

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
ESCRT-II

Plasmid

#17633

Depositor

James Hurley

Article

Hierro et al Nature. 2004 Sep 9. 431(7005):221-5.

Insert

pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567) (VPS36, SNF8, VPS25 Budding Yeast)

Expression

Bacterial

Available Since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

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pET21a.Tat(48-57)-I-SceI

Plasmid

#165041

Purpose

Expression of Tat(48-57) conjugated I-SceI for protein purification (6XHis tag)

Depositor

Dokyun Na

Article

Lee et al Commun Biol. 2021 Feb 15;4(1):205. doi:

Insert

I-SceI

Tags

6XHis and Tat(48-57)

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pHES836

Plasmid

#89195

Purpose

Expresses mKate2 under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-mKate2

Expression

Yeast

Promoter

pZ

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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p404-Grx5-GFP1-10

Plasmid

#91952

Purpose

Expresses GFP1-10 in the mitochondrial matrix

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

Grx5-GFP1-10

Expression

Yeast

Promoter

GAP

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pHACK(Gal4)-DONR(T2A-Flp1)

Plasmid

#194770

Purpose

To insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.

Depositor

Chun Han

Article

Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

Insert

T2A-Flp1

Use

CRISPR

Expression

Insect

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET21c-eIF4A

Plasmid

#37230

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF1 (TIF1 Budding Yeast)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pSLIK 3XFLAG-EGFP-NLS hygro

Plasmid

#87781

Purpose

Inducible expression of EGFP with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

eGFP

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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M4755 KanMX::LEU2 Disruptor Converter

Plasmid

#51685

Purpose

Yeast marker swap plasmid for converting KanMX3 to LEU2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LEU2 (LEU2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pMAL728

Plasmid

#169152

Purpose

Encodes OptoQ-AMP6

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Mutation

A79Q

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pZZMP154

Plasmid

#231760

Purpose

Mammalian expression of LZ-SPO11

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

LZ-SPO11 (Spo11 Budding Yeast, Mouse)

Tags

Flag-LeucineZipper

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSMF2

Plasmid

#112819

Purpose

Expresses wild-type Rpb1 in yeast cells

Depositor

Stephen Fuchs

Article

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Promoter

Native promoter

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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LHP306 - Ub - no Lys

Plasmid

#32150

Depositor

Linda Hicke

Article

Terrell et al Mol Cell. 1998 Jan;1(2):193-202.

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Expression

Yeast

Mutation

No Lys = K6R, K11R, K27R, K29R, K33A, K48R, K63R

Promoter

CUP1

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pJAT27

Plasmid

#204308

Purpose

For generating targeting inversions. C-terminal split GFP, expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-GFPc

Use

CRISPR

Promoter

MHC

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJAT18

Plasmid

#204307

Purpose

For generating targeting inversions. N-terminal split GFP, expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-GFPn

Use

CRISPR

Promoter

MHC

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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MIG(F)-RelA

Plasmid

#26986

Depositor

Stephen Smale

Article

Sanjabi et al Genes Dev. 2005 Sep 15. 19(18):2138-51.

Insert

RelA (Rela Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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pFH102

Plasmid

#128207

Purpose

sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRS426-GFP-Osh2-PH

Plasmid

#71373

Purpose

For analysis of PH domain localization in yeast

Depositor

Mark Lemmon

Article

Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

Insert

Osh2-PH

Tags

EGFP

Expression

Yeast

Mutation

Pleckstrin homology (PH) domain; aa 277-398

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only