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pCS2-TagRFPT.zf1

Plasmid

#61390

Purpose

mRNA synthesis of TagRFPT codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

TagRFPT.zf1

Use

Zebrafish expression

Promoter

sp6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pJAM1.19_PB-CIT-PGK-Blast

Plasmid

#205564

Purpose

Express human CIT

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

CIT (CIT Human)

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO5.sgRNA.EFS.tRFP657

Plasmid

#57824

Purpose

Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

Depositor

Benjamin Ebert

Article

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

sgRNA
EFS
tagRFP657

Use

CRISPR and Lentiviral

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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PB-ReNL

Plasmid

#123138

Purpose

Piggybac transposon plasmid for live animal optical imaging of Red-enhanced nanolantern

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Red-enhanced Nanolantern

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJDC181

Plasmid

#60853

Purpose

Expresses click beetle red luciferase

Depositor

Jeffrey Cirillo

Article

Chang et al PLoS One. 2014 Sep 29;9(9):e108341. doi:

Insert

Click beetle red luciferase (optimized for Mycobacteria)

Expression

Bacterial

Mutation

codon optimized for Mycobacteria

Promoter

hsp60

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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HSV-DYN-hM4Di

Plasmid

#53327

Purpose

Dynorphin promoter driving hM4Di

Depositor

John Neumaier

Article

Ferguson et al Nat Neurosci. 2011 Jan;14(1):22-4. doi:

Insert

hM4Di (Pdyn Rat)

Use

Hsv

Tags

3xHA tag

Expression

Mammalian

Promoter

Rat dynorphin promoter ( -1858 to +135)

Available Since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pTom20-mScarlet3-H (aka mYongHong)

Plasmid

#239412

Purpose

Expresses Tom20-mScarlet3-H in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

Tom20 (TOMM20 Human)

Tags

mScarlet3-H (aka mYongHong)

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCVD364

Plasmid

#63739

Purpose

V cholerae 395 nanH gene cloned into the BamHI site of pCVD315

Depositor

James Kaper

Article

Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.

Insert

nanH

Expression

Bacterial

Available Since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
p16L1-GFP

Plasmid

#89910

Purpose

Self-packages in TT-16L2 stable line. L1-only capsid propagation.

Depositor

John Schiller

Article

Buck et al J Virol. 2005 Mar;79(5):2839-46.

Insert

HPV16L1+ GFP

Use

Unspecified

Promoter

SV40 and hEF1alpha

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-GFP26

Plasmid

#106406

Purpose

P-RAN-GFP26 (RANbody GFP26::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-GFP26

Tags

HA and His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (AAV9)

Viral Prep

#100835-AAV9

Purpose

Ready-to-use AAV9 particles produced from pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 (#100835). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only
pTSAR3.4t

Plasmid

#210256

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

mCherry
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pORTMAGE-Ec1

Plasmid

#138474

Purpose

Enables high-efficiency recombineering in E. coli

Depositor

George Church

Article

Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

Inserts

CspRecT
mutL E32K

Expression

Bacterial

Mutation

E32K

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcdna nesprin TS

Plasmid

#68127

Purpose

nesprin tension sensor

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

mouse nesprin mini 2G (Syne2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-TFR1

Plasmid

#176490

Purpose

For lentiviral transduction of 3FLAG-TFR1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

TFR1 (TFRC Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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B2088_BTB-IDR-FRB

Plasmid

#215101

Purpose

Expresses BCL6 BTB(1-129)-mCherry-FUS (IDR)-FRB in mammalian cells

Depositor

Chandra Tucker

Article

Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

Insert

BTB-mCherry-FUS(IDR)-FRB

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-Mito-7

Plasmid

#57148

Purpose

Localization: Mitochondria, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

Mito (COX8A Human)

Tags

mPA-GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Tol2_ISCro4_Deletion_Reporter

Plasmid

#251498

Purpose

Reporter plasmid for monitoring deletion activity of ISCro4 bridge recombinase in the presence of native bridge RNA or native TBL and DBL components

Depositor

Martin Jinek

Article

Pelea et al Science. 2026 Feb 5:eadz1884. doi: 10.11

Insert

ISCro4 deletion reporter

Expression

Mammalian

Mutation

NA

Available Since

Feb. 6, 2026

Availability

Academic Institutions and Nonprofits only

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pOpen-Pfulig

Plasmid

#165525

Purpose

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Pfu DNA Ligase

Use

Synthetic Biology

Available Since

Aug. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pTRKH2

Plasmid

#71312

Purpose

Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Article

O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

Type

Empty backbone

Use

E. coli-gram positive bacteria shuttle vector

Expression

Bacterial

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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