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JBEI-6822

Plasmid

#84985

Purpose

For expression of pBbA5c-MevTaco-MKco-PMKco(aka 2A) under lacUV5 promoter for isopentenol production

Depositor

Taek Soon Lee

Publication

George et al Sci Rep. 2015 Jun 8;5:11128. doi: 10.103

Insert

MevTaco-MKco-PMKco

Expression

Bacterial

Promoter

LacUV5

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pHK

Plasmid

#64187

Purpose

harbours marker genes HIS3 and LYS2

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pHU

Plasmid

#64172

Purpose

harbours marker genes HIS3 and URA3

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLK

Plasmid

#64186

Purpose

harbours marker genes LEU2 and LYS2

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pUK

Plasmid

#64185

Purpose

harbours marker genes URA3 and LYS2

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pETa(+) HpCsd3 (42-403)

Plasmid

#71285

Purpose

Expresses Csd3 from Helicobacter pylori

Depositor

SeWon Suh

Publication

An et al Acta Crystallogr D Biol Crystallogr. 201

Insert

HP0506 (HP0506 )

Tags

His tag

Expression

Bacterial

Promoter

T7

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pRS424-URR

Plasmid

#65330

Purpose

high copy number plasmid as receiving vector for exogenous pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR1-RFP-Leu2-URR2

Use

Synthetic Biology

Available since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pRS414-URR

Plasmid

#65329

Purpose

CEN plasmid as receiving vector for exogenous pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

URR1-RFP-Leu2-URR2

Use

Synthetic Biology

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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MCEE

Plasmid

#39082

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MCEE (MCEE Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
MMAA

Plasmid

#39033

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

MMAA (MMAA Human)

Tags

His Tev

Expression

Bacterial

Available since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pCAG-ITGAM POD-nAb-WPRE

Plasmid

#244975

Purpose

Expresses a fusion protein of a nanobody against integrin alpha M (ITGAM; CD11b) and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Publication

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

ITGAM POD-nAb

Available since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-RFP6 POD-nAb-WPRE

Plasmid

#244974

Purpose

Expresses a fusion protein of a nanobody against RFP and peroxidase (POD) in mammalian cells

Depositor

Hiroyuki Hioki

Publication

Yamauchi et al Commun Biol. 2025 Jun 18;8(1):903. doi:

Insert

RFP6 POD-nAb

Available since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2678

Plasmid

#241723

Purpose

Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BLE marker for selection on phleomycin

Depositor

Hiten Madhani

Publication

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBHM2680

Plasmid

#241725

Purpose

Construction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSR marker for selection on blasticidin S

Depositor

Hiten Madhani

Publication

Boucher et al Microbiol Spectr. 2025 Sep 25:e0193525.

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Sept. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRpuro-sgROSA26

Plasmid

#231562

Purpose

pLentiCRISPR expression vector for Cas9 and gRNA targeting human ROSA26 locus (Puromycin selection marker)

Depositor

Rizwan Haq

Publication

Schiantarelli et al Cancer Cell. 2025 Feb 10;43(2):308-316.e

Insert

sgROSA26

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF-1a

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHEE401_GV_MOD

Plasmid

#194874

Purpose

mCherry selection in Arabidopsis seeds in T1 and T2

Depositor

Wolfgang Busch

Publication

Ruiz Rosquete et al Plant J. 2025 Jan;121(1):e17177. doi: 10

Insert

Cas9

Use

CRISPR

Promoter

egg cell-specific

Available since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_his6_sumo_229E_NSP14

Plasmid

#213521

Purpose

Expresses NSP14 of hCoV 229E

Depositor

Thomas Tuschl

Publication

Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

Insert

hCoV 229E NSP14

Tags

His6-sumo

Expression

Bacterial

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER

Plasmid

#222189

Purpose

Bacterial expression of mouse PTER protein

Depositor

Jonathan Long

Publication

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Available since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-p18-mCherry-LOVPLD

Plasmid

#219560

Purpose

lysosome-targeted LOVPLD

Depositor

Jeremy Baskin

Publication

Li et al ACS Cent Sci. 2024 Jan 30;10(3):543-554.

Insert

p18-mCherry-LOVPLD

Tags

mCherry

Expression

Mammalian

Available since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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GW1-mCherryEA

Plasmid

#207867

Purpose

Mammalian expression (cytosol) of the mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

Depositor

Mathew Tantama

Publication

Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

Insert

mCherryEA

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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