We narrowed to 33,357 results for: Sis

1. hHSF1 K298R

   Plasmid

   #118362

   Purpose

   This plasmid expresses human HSF1 K298R mutant, which cannot undergo sumoylation on K298.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K298R

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PDZb-beta2(616-951)-mCherry

   Plasmid

   #100736

   Purpose

   To express a clathrin "hook" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   AP2B1 (AP2B1 Human)
   

   Use

   Tags

   PDZb and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICE-HA-CtIP-siR-WT

   Plasmid

   #82030

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Patrick Calsou, Sébastien Britton

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   CtIP (RBBP8 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Mutations making the cDNA resistant to a siRNA

   Promoter

   CMV-tet

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRE-Tight-FLAG-CYLD-C601A

   Plasmid

   #60029

   Purpose

   CYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Cysteine 601 changed to Alanine

   Promoter

   TRE

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. GFP-CYLD-C601A

   Plasmid

   #60028

   Purpose

   N-terminal GFP tagged catalytic inactive (C601A) expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Cysteine 601 changed to Alanine

   Promoter

   CMV

   Available since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUPER-hHSF2 shRNA

   Plasmid

   #118346

   Purpose

   This plasmid expresses shRNA against hHSF2.

   Depositor

   Lea Sistonen

   Article

   Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

   Insert

   hHSF2 shRNA (HSF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR4 PARP2 E614Q

   Plasmid

   #192545

   Purpose

   pENTR plasmid Arabidopsis thaliana PARP2 CDS, E614Q mutation, no stop codon

   Depositor

   Lennart Wirthmueller

   Article

   Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

   Insert

   poly(ADP-ribose) polymerase 2 E614Q (AT3G18250 Mustard Weed)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   changed Glu 614 to Gln

   Promoter

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR4 CDS A. thaliana PHOT1

   Plasmid

   #209187

   Purpose

   pENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tags

   Depositor

   Lennart Wirthmueller

   Article

   Simranjit et al bioRxiv 2023.09.25.558804

   Insert

   AT3G45780.1 (PHOT1 Mustard Weed)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Nov. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Susd4(3.7)-EGFP-sv40

   Plasmid

   #184413

   Purpose

   AAV expression of tTA from mouse sushi domain containing 4 (Susd4) gene promoter.

   Depositor

   Marylka Yoe Uusisaari

   Article

   Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

   Insert

   Mouse Susd4 gene promoter-EGFP (Susd4 Mouse)
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Susd4 gene

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWZLblasti-HA-MEK1

    Plasmid

    #53161

    Purpose

    Expresses HA tagged human MEK1 from blasticidin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pLVX-mCherry-SPASTIN, F124D

    Plasmid

    #180648

    Purpose

    Lentiviral expression vector for SPASTIN. Has F124D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-44.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Starts at M87, F124D mutation, has siRNA resistan…

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. His-DNPH1

    Plasmid

    #203224

    Purpose

    For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

    Depositor

    Vern Schramm

    Article

    Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

    Insert

    DNPH1 (DNPH1 Human)
    

    Use

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1α-M2rtTA-T2A-Puro

    Plasmid

    #169175

    Purpose

    piggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174

    Depositor

    Igor Slukvin

    Article

    Jung et al Cell Rep. 2021 Feb 16;34(7):108758. doi:

    Insert

    M2rtTA-T2A-Puro
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEXfrt-SaCas9-U6-sgGabrg1

    Plasmid

    #124870

    Purpose

    Mutagenesis of Gabrg1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg1 (Gabrg1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-AtTAS1c-D2-B/c

    Plasmid

    #137883

    Purpose

    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]

    Depositor

    Alberto Carbonell

    Article

    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

    Insert

    AtTAS1c-D2-B/c
    

    Use

    Gateway-compatible entry clone

    Tags

    Expression

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Promoter

    Available since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p221- purVSG117UTR

    Plasmid

    #59732

    Purpose

    Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

    Depositor

    Gloria Rudenko

    Article

    Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

    Insert

    VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site
    

    Use

    For homologous recombination into vsg221 expressi…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-FLEX-SaCas9-U6-sgGabrg1

    Plasmid

    #124856

    Purpose

    Mutagenesis of Gabrg1

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg1 (Gabrg1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPKm-245

    Plasmid

    #90503

    Purpose

    Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - P2A - MTS - tFD - P2A - MTS - tFNR

    Depositor

    Todd Coleman

    Article

    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

    Insert

    mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

    Plasmid

    #124864

    Purpose

    Mutagenesis of Grin2b

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2b (Grin2b Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEE048

    Plasmid

    #176805

    Purpose

    Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

    Insert

    504
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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