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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 4881 - 4900 of 16276 results

  2. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  3. pCMV_T7-PE2-Nuclease

    Plasmid
    #171997
    Purpose
    Sequence provided for PE-Nuclease mRNA transcription, suitable for microinjection
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CMV_T7-Cas9-RT
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171997-plasmid-map-sequence-id-338159

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  4. mCh-KIF1A*-strep

    Plasmid
    #120165
    Purpose
    Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    kinesin family member 1A (Kif1a Mouse)
    Tags
    Streptavidin and mCherry
    Expression
    Mammalian
    Mutation
    Truncated KIF1A: 1-405 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    120165-plasmid-map-sequence-id-251908

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  6. f-CTS-EGFP

    Plasmid
    #86064
    Purpose
    A mammalian expression plasmid encoding fibrocystin ciliary targeting signal domain with C-terminus EGFP.
    Depositor
    Lei Lu
    Publication
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains ciliary targeting signal (3870-3887 amin…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86064-plasmid-map-sequence-id-179754

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  11. Hsp68-LacZ-Gibson

    Plasmid
    #170102
    Purpose
    Transgenic LacZ reporter construct for validation of enhancer sequences (inserted via Gibson-cloning). Can be introduced into the mouse genome via random integration following pro-nuclear injection.
    Depositor
    Len Pennacchio
    Type
    Empty backbone
    Use
    Mouse Targeting
    Promoter
    Mouse Hsp68 minimal promoter
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    170102-plasmid-map-sequence-id-346308

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  12. mPA-Emerald-N1

    Plasmid
    #54574
    Purpose
    Localization: N1 Cloning Vector, Excitation: 400 / 487, Emission: 509
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mPA-Emerald
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. mPA-Emerald-C1

    Plasmid
    #54687
    Purpose
    Localization: C1 Cloning Vector, Excitation: 400 / 487, Emission: 509
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    mPA-Emerald
    Expression
    Mammalian
    Available since
    June 11, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 4881 - 4900 of 16276 results