Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 19,993 results for: nls

Clear keywords
Showing: 4881 - 4900 of 19993 results

  1. G9A[SET]-dCas9

    Plasmid
    #100089
    Purpose
    Catalytic domain [SET] of human G9A fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G9A (EHMT2 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-G9A[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    G9A[SET] includes aa 829-1209
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100089-plasmid-map-sequence-id-197561

    Enlarge

  2. pWS171

    Plasmid
    #90518
    Purpose
    Cas9 gap repair vector - LEU2
    Depositor
    Tom Ellis
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Oct. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90518-plasmid-map-sequence-id-288271

    Enlarge

  4. pDUAL SRBI (GFP)

    Plasmid
    #86979
    Purpose
    Lentiviral expression construct encoding SR-B1 and GFP from separate promoters
    Depositor
    Joe Grove
    Insert
    SCARB1 (SCARB1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    SFFV/hPGK
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    86979-plasmid-map-sequence-id-514761

    Enlarge

  5. Lenti-LbCpf1-Blast

    Plasmid
    #84751
    Purpose
    Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n
    Insert
    LbCpf1
    Use
    CRISPR and Lentiviral
    Tags
    NLS-3xHA (C terminal on insert)
    Expression
    Mammalian
    Promoter
    EFS-NS
    Available since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    84751-plasmid-map-sequence-id-164465

    Enlarge

  6. pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

    Plasmid
    #69138
    Purpose
    For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo
    Depositor
    Takuji Iwasato
    Publication
    Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
    Tags
    none
    Expression
    Mammalian
    Available since
    Nov. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69138-plasmid-map-sequence-id-165113

    Enlarge

  9. pRubiC-T2A-Cas9

    Plasmid
    #75347
    Purpose
    Ubiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.
    Depositor
    Bryan Luikart
    Publication
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Insert
    Cas9
    Use
    CRISPR and Retroviral
    Tags
    mCherry (via t2a)
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available since
    May 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    75347-plasmid-map-sequence-id-232926

    Enlarge

  10. PXL

    Plasmid
    #75349
    Purpose
    PX330 derived. Bbs1 & annealed oligos to insert guide strand. BstB1+Pac1 of PXL and FUX-/pRubiX- T2A-Cas9 for choice of sgRNA, viral backbone, and fluorescent protein. Pac1 to introduce 2nd sgRNA.
    Depositor
    Bryan Luikart
    Publication
    Williams et al Sci Rep. 2016 May 10;6:25611. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    May 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75349-plasmid-map-sequence-id-266810

    Enlarge

  15. pRSV-PKI-v2

    Plasmid
    #45066
    Purpose
    Expression of PKI (PKA inhibitor)
    Depositor
    Richard Maurer
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    cAMP-dependent protein kinase inhibitor alpha
    Expression
    Mammalian
    Promoter
    RSV
    Available since
    July 31, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    45066-plasmid-map-sequence-id-62500

    Enlarge

Showing: 4881 - 4900 of 19993 results