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We narrowed to 14,582 results for: eng

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Showing: 4901 - 4920 of 14582 results

  1. pKan cpRBP-FL P53

    Plasmid
    #174504
    Purpose
    Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)
    Depositor
    Stewart Loh
    Publication
    Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do
    Insert
    P53 gene (TP53 Human)
    Tags
    20 amino acid linker containing HRV3C protease si…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174504-plasmid-map-sequence-id-344828

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  2. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid
    #129028
    Purpose
    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-hudTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A), catalytic domain huTET1 inact…
    Promoter
    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129028-plasmid-map-sequence-id-256131

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  3. pZE21/UBP1/ClpS

    Plasmid
    #98566
    Purpose
    Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon
    Depositor
    George Church
    Publication
    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11
    Inserts
    Truncated ubiquitin cleavase, codon optimized
    ATP-dependent Clp protease adapter protein
    Expression
    Bacterial
    Mutation
    In a synthetic operon downstream of UBP1 and Remo…
    Promoter
    Same as UBP1 (operon) and Tet promoter (aTc induc…
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98566-plasmid-map-sequence-id-203067

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  4. p28-2iDP-puro

    Plasmid
    #88893
    Purpose
    Dual-intron DMD platform plasmid. Co-expresses DMD platform segment, firefly luciferase, puroR and mCherry. Plasmid carries phiC31 and Bxb1 attB sites.
    Depositor
    Michele Calos
    Publication
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Dual-intron DMD platform
    luciferase
    puromycin resistance enzyme
    mCherry
    Expression
    Mammalian
    Mutation
    Truncated version of the dystrophin protein (tran…
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    88893-plasmid-map-sequence-id-201484

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  7. Dendra2-NS3

    Plasmid
    #238004
    Purpose
    Expresses NS3 in mammalian/mouse cell line
    Depositor
    Partha Mondal
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NS3
    Tags
    Dendra2
    Expression
    Mammalian
    Available since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    238004-plasmid-map-sequence-id-474227

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  8. pCAG-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1060)

    Plasmid
    #114093
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548V and N552R
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114093-plasmid-map-sequence-id-230492

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  11. pSems-leader-SNAPf-mEGFPe-IFNAR1(28-557)

    Plasmid
    #187844
    Purpose
    Expression of SNAPf and mEGFPe-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy
    Depositor
    Jacob Piehler
    Publication
    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    IFNAR1 (IFNAR1 Human)
    Tags
    Ig k-chain leader sequence, SNAPf-tag, and meGFPe…
    Expression
    Mammalian
    Mutation
    meGFPe: asparagine 198 to aspartic acid and tyros…
    Promoter
    CMV
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    187844-plasmid-map-sequence-id-379371

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  12. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4

    Plasmid
    #193345
    Purpose
    tetO-OS*K (Dox-inducible reprogramming lentiviral OSK vector expressing mouse Oct4 + super-Sox + Klf4)
    Depositor
    Hans Schöler
    Publication
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Insert
    Oct4-p2a-Sox2-17-t2a-Klf4 (Klf4, Pou5f1, Sox2 Mouse)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    TetO mini CMV
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    193345-plasmid-map-sequence-id-410597

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  14. pCfB6682

    Plasmid
    #106152
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Publication
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106152-plasmid-map-sequence-id-205326

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  15. pCfB6684

    Plasmid
    #106154
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Publication
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106154-plasmid-map-sequence-id-206237

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Showing: 4901 - 4920 of 14582 results