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NAX_CAGGS_CAS9_EBFP2

Plasmid

#167857

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCS4083

Plasmid

#131739

Purpose

Two Color Control Assay bicistronic BFP-mCherry-sTRSVctl

Depositor

Christina Smolke

Publication

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

sTRSV

Expression

Mammalian

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pWS481

Plasmid

#123029

Purpose

LexA UAS

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

LexO (6x) [2a]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBSPPc

Plasmid

#116918

Purpose

Gene constitutive expression from a Pc promoter

Depositor

Cuiqing Ma

Publication

Xu et al Appl Environ Microbiol. 2013 Apr;79(8):2

Type

Empty backbone

Expression

Bacterial

Promoter

Pc promoter from Delftia acidovorans C17

Available since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pANY6

Plasmid

#112198

Purpose

This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.

Depositor

Yingfeng An

Publication

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Tags

ELP-tag

Expression

Bacterial

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pUX19

Plasmid

#36328

Depositor

Gary Roberts

Type

Empty backbone

Use

Suicide vector for r. rubrum

Expression

Bacterial

Promoter

reduce strength lacZ

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
p356_DG

Plasmid

#234699

Purpose

Golden gate domesticated pET28b for scarless cloning with His10-MBP-3C

Depositor

Matthew Wohlever

Publication

Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

Type

Empty backbone

Tags

His10-MBP-3C

Expression

Bacterial

Mutation

No

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcS-p88-C1C2

Plasmid

#178424

Purpose

p88 as a potential β-cell (pancreatic tissue) targeting peptide fused to EV membrane binding domain

Depositor

Masako Harada

Publication

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Insert

p88

Expression

Mammalian

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-gLuc-C1C2

Plasmid

#178426

Purpose

Bioluminescence reporter fused to EV membrane binding domain for EV imaging and tracking

Depositor

Masako Harada

Publication

Komuro et al Nanotheranostics. 2021 Apr 15;5(4):378-3

Insert

gLuc

Expression

Mammalian

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAGM53151

Plasmid

#169093

Purpose

Level 1 cloning vector for transient expression. Already contains the 35S promoter and the Nos terminator.

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Type

Empty backbone

Use

Moclo cloning vector

Expression

Plant

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ143-ZmUbi

Plasmid

#138107

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoter

Depositor

Yiping Qi

Publication

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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p33Lb

Plasmid

#158156

Purpose

Expression of crRNA array and LbCas12a using one Pol II promoter. For plant genome editing.

Depositor

Kabin Xie

Publication

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB6631

Plasmid

#106161

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6684 into Yarrowia lipolytica chromosomal location IntD_1, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB6630

Plasmid

#106160

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6371 into Yarrowia lipolytica chromosomal location IntC_3, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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1xNLS-pMJ915v2

Plasmid

#88915

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Publication

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVV210

Plasmid

#87628

Purpose

Drives Bxb1 expression in the D. melanogaster germ line.

Depositor

Richard Mann

Publication

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1

Expression

Insect

Promoter

vasa

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRVV601

Plasmid

#87632

Purpose

RMCE Bxb1 attB-flanked vector.

Depositor

Richard Mann

Publication

Voutev et al Biotechniques. 2017 Jan 1;62(1):37-38. d

Insert

Bxb1 attB

Use

Rmce

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGGC012

Plasmid

#48826

Purpose

Provides GFP:NLS as GreenGate coding sequence module.

Depositor

Jan Lohmann

Publication

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

GFP:NLS

Use

Golden gate compatible cloning vector

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-haA3A-CBE-G

Plasmid

#220894

Purpose

Express haA3A-CBE-G (with SpCas9) in mammalian cells

Depositor

Dali Li

Publication

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G

Expression

Mammalian

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pECO200

Plasmid

#169765

Purpose

Express SpCas9 and Basta resistance gene

Depositor

Sang-Gyu Kim

Publication

Oh et al Plant Methods. 2020 Mar 12;16:37. doi: 1

Type

Empty backbone

Use

Plant binary vector

Expression

Plant

Promoter

35S, AtU6

Available since

July 16, 2021

Availability

Academic Institutions and Nonprofits only

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